DEAD-box helicase 1
Involvement of a Toxoplasma DEAD-box RNA Helicase in Post-transcriptional Gene Regulation
... of helicase superfamilies (SFs) based on their core domains Hexameric RecA-like domain: SF4 and SF5 helicases contain six units of RecA- like domains, which are homologous to the bacterial recombinase-A (RecA) ...
121
SACY-1 DEAD-Box Helicase Links the Somatic Control of Oocyte Meiotic Maturation to the Sperm-to-Oocyte Switch and Gamete Maintenance in Caenorhabditis elegans
... By contrast, SACY-1 might function in post-transcriptional gene regulation important for the control of oocyte meiotic maturation, the hermaphrodite sperm-to-oocyte switch, and gamete maintenance. Genetic ...
69
DDX3 DEAD-Box RNA Helicase Inhibits Hepatitis B Virus Reverse Transcription by Incorporation into Nucleocapsids
... Plasmids. All DNA constructs were generated by overlap extension PCR protocols as described previously (23). The details of the molecular cloning of any plasmid construct will be provided upon request. The HBV overlength ...
10
Regulation of Notch Signaling by an Evolutionary Conserved DEAD Box RNA Helicase, Maheshvara in Drosophila melanogaster
... with 1 mM IPTG, purified on a glutathione sepharose column (GE Healthcare) per manu- facturer’s instructions, and then used for raising polyclonal antibody in ...(Amersham; 1:2000) and color were developed ...
21
The DEAD box RNA helicase Vad1 regulates multiple virulence associated genes in Cryptococcus neoformans
... The finding of a role for an RCK/p54 protein in the regulation of PCK1 has not, to our knowledge, been reported previously and sug- gests an additional regulatory pathway for this enzyme in addition to the cAMP and ...
11
The Sole DEAD Box RNA Helicase of the Gastric Pathogen Helicobacter pylori Is Essential for Colonization
... tiple DEAD-box RNA helicases that have versatile and partially overlapping ...4 DEAD-box helicases, respectively. In many bacteria, DEAD-box helicases are involved in adaptation ...
17
The DEAD box RNA helicase Vasa functions in embryonic mitotic progression in the sea urchin
... We found that Vasa was dynamically expressed in all cells of the early embryo in a cell-cycle dependent manner (Fig. 1A). Vasa accumulated peri-nuclearly at prophase and became enriched around the developing aster (Fig. ...
6
DDX3 DEAD-Box RNA Helicase Is Required for Hepatitis C Virus RNA Replication
... on 30 l of protein G-Sepharose resin for 1 h, the resin was washed four times with 700 l lysis buffer. Proteins were eluted by boiling the resin for 5 min in 1 ⫻ Laemmli sample buffer. The proteins were ...
5
Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma
... TMA blocks were cut into 4-μm paraffin sections, dewaxed in xylene, rehydrated through a series of graded alcohols, and subsequently washed for 5 minutes with phosphate-buffered saline (PBS) [50]. The Novolink max ...
12
Alterations in the expression of DEAD box and other RNA binding proteins during HIV 1 replication
... the helicase family at the ATP binding site [31], indicat- ing that many ATP-dependent processes may be targeted by early viral replication steps, not only as a means to facilitate viral RNA transport but also as ...
5
SpolvlgA is a DDX3/PL10-related DEAD-box RNA helicase expressed in blastomeres and embryonic cells in planarian embryonic development
... SpolvlgA expression in adult organisms was monitored by in situ hybridization and fluorescent in situ hybridization on paraffin sections. We found that the expression of SpolvlgA could be dectected in testis easily (Fig. ...
10
An Essential Role for the Saccharomyces cerevisiae DEAD-Box Helicase DHH1 in G1/S DNA-Damage Checkpoint Recovery
... Figure 1.—DHH1 is the ortholog of p54/RCK and is required for resistance to DNA damage. (A) Viability after exposure to UV irradiation and growth on YPD plates containing 0.015% MMS were measured in YJR218 (DHH1) ...
14
DDX3 DEAD-Box RNA Helicase Is a Host Factor That Restricts Hepatitis B Virus Replication at the Transcriptional Level
... DDX3 downregulates HBV RNA via transcriptional regula- tion. DDX3 has been implicated in the transcriptional regulation of several promoters (14–16). Thus, it is possible that DDX3 in- hibits viral RNA synthesis by ...
10
Centroid, a novel putative DEAD box RNA helicase maternal mRNA, is localized in the mitochondrial cloud in Xenopus laevis oocytes
... a 1 6 9 PS S I C NRS LLVS A D T G S G K T ASF LVPIIA H C S H V R S E R C T D - K Q GPLAI VLAP T R E LC LQVEEQ A KVLG KG L P- F KTALVV G GDP LA QQI YRI E N G I E LIV GT P G R LIDL L M K H NVDLN K V D V FVLD EVDC ...
7
DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells
... For the sphere-formation assay, cells were cultured in stem cell medium (SCM) containing DMEM/F-12 (Thermo Fisher Scientific), B-27 supplement (1:50; Thermo Fisher Scientific), 20 ng/mL human epidermal growth ...
9
Conserved requirement for DEAD box RNA helicase Gemin3 in Drosophila oogenesis
... Ovaries of female flies, which were fed on wet yeast for ~3 days in the presence of males, were dissected in PBS (phosphate buffered saline) at room temperature and later fixed in 4% paraformaldehyde in PBS. The tissue ...
9
In Silico Structural Characterization of Plasmodium Falciparum Helicase, PfBrr2
... Brr2 Helicase Region S1087L (PDB ID: 4F92) for Sec63 Brl II ...of DEAD box helicase was ...of Helicase C terminal conserved region ...
11
DEAD box helicase DP103 defines metastatic potential of human breast cancers
... The NF-κB family of transcription factors comprises 5 structur- ally related proteins that form homo- and heterodimers through their highly conserved DNA binding/dimerization Rel homology domain (32). Binding of NF-κB to ...
19
Identification of the DEAD box RNA helicase DDX3 as a therapeutic target in colorectal cancer
... All cells were harvested at 50–70% confluency. For DDX3 knockdown experiments cells were harvested 72 hours after transfection. For RK-33 experiments cells were harvested after 24 hours exposure to the drug or vehicle ...
15
The DEAD box RNA helicase Ddx39ab is essential for myocyte and lens development in zebrafish
... Fig. 1. Loss of ddx39ab leads to an embryonic lethal phenotype in zebrafish. (A) GFP expression pattern in zebrafish trapping line RP-011. Lateral views. S, somite. Arrowhead in inset indicates GFP expression in ...
12