DEAD box helicase 3
Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma
... First, the intensity score of the DDX60 staining was measured using a numerical scale (0, no expression; 1, weak expression; 2, moderate expression; and 3, strong expression; Figure 1A). The immunoreactivity of ...
12
Unexpected roles for DEAD box protein 3 in viral RNA sensing pathways
... the DEAD (Asp-Glu-Ala- Asp) box helicase DDX3 (DEAD/H Box 3), known for some time to have a number of roles in cellular RNA regulation in the nucleus, has a role in the RLR ...
9
SACY-1 DEAD-Box Helicase Links the Somatic Control of Oocyte Meiotic Maturation to the Sperm-to-Oocyte Switch and Gamete Maintenance in Caenorhabditis elegans
... (RNAi) yet exhibit normal RNAi responses and are viable and fertile. From this screen, only mutations from a single complementation group, represented by tn1237, tn1242, and tn1336, suppress acy-4(lf) sterility. One of ...
69
A HIV 1 Tat mutant protein disrupts HIV 1 Rev function by targeting the DEAD box RNA helicase DDX1
... BRU clone of HIV-1 Rev (pRSV-Rev) was a gift from Damian Purcell, The University of Melbourne, Australia. pcDNA3-HA, pcDNA3-HA-DDX1, pcDNA3-HA-DDX3 and pcDNA3-HA-DDX17 were obtained from Yasuo Ariumi, Kumamoto ...
17
The DEAD box RNA helicase Vad1 regulates multiple virulence associated genes in Cryptococcus neoformans
... virulence-associated DEAD-box RNA helicase–encoding gene (VAD1) was isolated from a mutant defective in the virulence fac- tor ...identify 3 additional virulence determinants dependent on ...
11
Regulation of Notch Signaling by an Evolutionary Conserved DEAD Box RNA Helicase, Maheshvara in Drosophila melanogaster
... novel DEAD box helicase mahe, which is predicted to comprise five exons and codes for a transcript that is ...conserved DEAD box helicase family, is 945 amino acids long (Figure ...
21
The DEAD box RNA helicase Vasa functions in embryonic mitotic progression in the sea urchin
... We surveyed the cell-cycle-dependent Vasa localization in other echinoderms and found that Vasa protein was localized in the nuclear region of cells in M phase with a strong morphological Fig. 3. The Vasa C ...
6
SpolvlgA is a DDX3/PL10-related DEAD-box RNA helicase expressed in blastomeres and embryonic cells in planarian embryonic development
... SpolvlgA expression was monitored by in situ hybridization both on whole mount embryos and on paraffin sections. We found that the expression of SpolvlgA could be detected as early as in stage 1 embryos (Fig. 3a-b, ...
10
DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells
... The shRNA lentiviral vectors used for knockdown of DDX27 were synthesized by Genepharma Company (Suzhou, China). The following shRNA sequences were used for the DDX27 knockdown experiment: shRNA1 (5 ′ -GCTTGCGGA ...
9
DDX3 DEAD-Box RNA Helicase Inhibits Hepatitis B Virus Reverse Transcription by Incorporation into Nucleocapsids
... subjected to OptiPrep density gradient centrifugation, and DDX3, HBV Pol, and the core protein in each fraction were detected by immunoblot analysis with the respective antibodies (Fig. 6A). The core protein was detected ...
10
An Essential Role for the Saccharomyces cerevisiae DEAD-Box Helicase DHH1 in G1/S DNA-Damage Checkpoint Recovery
... Figure 1.—DHH1 is the ortholog of p54/RCK and is required for resistance to DNA damage. (A) Viability after exposure to UV irradiation and growth on YPD plates containing 0.015% MMS were measured in YJR218 (DHH1) and ...
14
Centroid, a novel putative DEAD box RNA helicase maternal mRNA, is localized in the mitochondrial cloud in Xenopus laevis oocytes
... a 3 0 7 VM QIF Q A L S H P -QV M M FS A T V N S E VEK M S N SLA K N A I HIS CGN P S RPN - K SVKQVVIW V ES K QK KQKIF E I M T S K Q HF K P-PA VV F VS S R IG A D L LS EAI T V A TG LK V V SI HG DKT M NE RR E SLR R ...
7
DDX3 DEAD-Box RNA Helicase Is a Host Factor That Restricts Hepatitis B Virus Replication at the Transcriptional Level
... DDX3 inhibits both HBV replication and core protein expres- sion. We previously reported that ectopic expression of DDX3 suppresses HBV replication in HepG2 cells (24). However, the underlying mechanism of inhibition has ...
10
The Sole DEAD Box RNA Helicase of the Gastric Pathogen Helicobacter pylori Is Essential for Colonization
... the DEAD-box helicases distribution in the Epsilonpro- teobacteria class highlighted a striking feature: every member of the Campylobacter and Helicobacter genera, which included major pathogens such as ...
17
The DEAD box RNA helicase Ddx39ab is essential for myocyte and lens development in zebrafish
... Fig. 6. ddx39ab deficiency affects pre-mRNA splicing of epigenetic modulator genes. (A) The splicing status of kmt2a, kmt2ba, kmt2bb, kmt2ca, kmt2cb and actb1 pre-mRNA was monitored by RT-PCR with the primers illustrated ...
12
Involvement of a Toxoplasma DEAD-box RNA Helicase in Post-transcriptional Gene Regulation
... The transgenic TgHoDI-SG and TgHoDI-YFP strains exhibited similar doubling time in comparison to the parental strain, which showed an approximate replication time of approximately 8 hours for the two transgenic clones, ...
121
Conserved requirement for DEAD box RNA helicase Gemin3 in Drosophila oogenesis
... During oogenesis, nurse cells undergo 10-12 endocycles, which are modified cell cycles that alternate between the S (DNA synthesis) and G (gap) phase. During the first four endocycles, homologous chromosomes are paired ...
9
In Silico Structural Characterization of Plasmodium Falciparum Helicase, PfBrr2
... Brr2 Helicase Region S1087L (PDB ID: 4F92) for Sec63 Brl II ...(Fig 3) and these were evaluated using ERRAT and RAMPAGE (Fig 4 and Fig ...of DEAD box helicase was ...of Helicase ...
11
DEAD box helicase DP103 defines metastatic potential of human breast cancers
... nuclear and receptor-mediated NF-κB activation, which works by phosphorylating the Ser180/Ser181 in the activation loop of IKKs (39, 40, 44). We first investigated whether endogenous DP103 and TAK1 interact. Indeed, ...
19
Identification of the DEAD box RNA helicase DDX3 as a therapeutic target in colorectal cancer
... Apart from several studies finding an oncogenic role of DDX3 in cancer [3, 4, 6, 17–22], others have suggested that DDX3 may also have a tumor suppressive role in certain cancers. [23–26] Although it is difficult ...
15