Detergent-insoluble
p27Kip1 Localizes to Detergent-insoluble Microdomains Within Lymphocyte Membranes
... Solubilization of lymphocytes in cold NP-40 for 30 min resulted in the loss of nuclear p27 on exam- ination by confocal microscopy (Fig. 3). A rim of p27 remained in the membrane, however, suggesting decreased solubility ...
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Comparison of acid-detergent lignin, alkaline-peroxide lignin, and acid-detergent insoluble ash as internal markers for predicting fecal output and digestibility by cattle offered bermudagrass hays of varying nutrient composition
... Based on this information, ADIA and APL are potential internal markers that can predict FO and DMD of bermu- dagrass hay having a wide range of CP concentrations, while ADL does not appear to be useful for predicting ...
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Diurnal variation in fecal concentrations of acid-detergent insoluble ash and alkaline-peroxide lignin from cattle fed bermudagrass hays of varying nutrient content
... Background: The effect of time of fecal sampling on the accuracy of acid-detergent insoluble ash (ADIA) and alkaline-peroxide lignin (APL) for the prediction of fecal output (FO) in cattle was evaluated. ...
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A Subpopulation of Tegument Protein vhs Localizes to Detergent-Insoluble Lipid Rafts in Herpes Simplex Virus-Infected Cells
... largely insoluble in HSV-infected cells, even in the presence of high levels of salt and the detergent Triton ...with detergent-insoluble lipid rafts and this raft population is enriched in a ...
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pp60c-src has less affinity for the detergent-insoluble cellular matrix than do pp60v-src and other viral protein-tyrosine kinases.
... In summary, the protein-tyrosine kinase activities associated with the transforming proteins of FSV and Y73 ASV both were present in the DIM of infected cells, as had previously been obs[r] ...
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Localization of the feline sarcoma virus fgr gene product (P70gag-actin-fgr): association with the plasma membrane and detergent-insoluble matrix.
... 66-72 0022-538X/86/070066-07$02.00/0 Copyright © 1986, American Society for Microbiology Localization of the Feline Sarcoma Virus fgr Gene Product P70gag-actin-fgr: Association with the [r] ...
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Localization of the Abelson murine leukemia virus protein in a detergent-insoluble subcellular matrix: architecture of the protein.
... Lanes 1, 4, and 5, labeled 2M3 cell extract, labeled 2M3 cell extract plus unlabeled ANN-I ceU extract, and labeled ANN-1 cell extract, respectively, all immunoprecipitated with normal g[r] ...
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Activation of tyrosine kinase c Abl contributes to α synuclein–induced neurodegeneration
... IP and IB analysis. For coimmunoprecipitation from cell cultures, SH-SY5Y cells were transiently transfected with indicated plasmids. After 48 hours, cells were washed with cold PBS and harvested in IP buffer (1% Triton ...
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Wild type human TDP-43 potentiates ALS-linked mutant TDP-43 driven progressive motor and cortical neuron degeneration with pathological features of ALS
... in detergent insoluble TDP-43 compared to age-matched non-transgenic animals ...of detergent insoluble TDP-43 and the degenerative pathological and behavioural motor phenotype observed in ...
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Vesiculation and rafts in stomatocytic red cells
... 1 vol red cell membranes prepared without EDTA were subject to solubilization in 1 vol ice- cold Triton X-100 at different concentrations (v/v), sonicated for 30 seconds and made 45% in sucrose, then centrifuged under a ...
347
Convergence and Divergence of the Signaling Pathways for Insulin and Phosphoinositolglycans
... Addition of increasing amounts of trypsin/ salt extract prepared from detergent-insoluble complexes to trypsin/salt-treated adipocytes and subsequent reconstitution led to restoration of[r] ...
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Validation of metabolisable protein and energy systems to predict the productivity of meat goats fed tropical grass, legumes and protein supplements
... List of Abbreviations Abbreviation Name ADG Average daily gain ADIN Acid detergent insoluble nitrogen AFRC Agricultural and Food Research Council ATP Adenosine triphosphate BW Body weigh[r] ...
212
Vaccinia Virus Penetration Requires Cholesterol and Results in Specific Viral Envelope Proteins Associated with Lipid Rafts
... isolated detergent-insoluble glycolipid-enriched membranes from cells immediately after virus infection and demon- strated that several viral envelope proteins, A14, A17L, and D8L, were present in the cell ...
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Ruminal degradability of Guinea grass silage inoculated with Streptoccocus bovis isolated from bovine rumen combined or not with com wheat bran
... It was determined the Silages degradation of dry mat- ter (DM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) (Nocek [17]). In the estimation of kinetic parameters of DM ...
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Characterization, Partial Purification of Alkaline Protease from Intestinal Waste of Scomberomorus Guttatus and Production of Laundry Detergent with Alkaline Protease Additive
... in detergent even after 1 hour of ...laundry detergent with enzyme additive was produced and the de-staining activity of the newly prepared detergent with enzyme additive was promising and thus can ...
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Usage and recall of FMCG brands by rural consumers and relevant facts
... The success of a brand in the Indian rural market is as unpredictable as rain. It is always difficult to gauge the rural market. Many brands, which should have been successful, have failed miserably in rural markets ...
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Effect of viral RNase H on the avian sarcoma viral genome during early transcription in vitro.
... To determine whether RNase H activity itself depends directly on the detergent concentration, we measured the influence of various detergent concentrations on the ability of RNase H to d[r] ...
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Analysis of detergent-free lipid rafts isolated from CD4+ T cell line: interaction with antigen presenting cells promotes coalescing of lipid rafts
... the detergent-free case, fre- quency of macrodomain formation did not depend on the presence of antigen (Figure ...to detergent isolation of lipid ...that detergent does not affect the average size ...
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Response of Maize Forage Yield and Quality to Nitrogen Fertilization and Harvest Time in Semi−arid Northwest China
... study increased. Acid detergent fiber is an appropriate index to determine forage digestibility whereas NDF in the feed is an indication of cell wall quantity [25]. In the current study, nitrogen fertilization ...
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Crossed immunoelectrophoretic studies of the solubility immunogenicity of herpes simplex virus antigens.
... The purpose of the present study was to measure to what extent HSV-infected cells are solubilized with and without detergent, to identify water-soluble and detergent-requiring viral anti[r] ...
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