Diarrheagenic E. coli
High prevalence of diarrheagenic Escherichia coli carrying toxin encoding genes isolated from children and adults in southeastern Brazil
... of diarrheagenic E. coli by multiplex PCRs All E. coli isolates were subjected to two multiplex PCRs, as previously described, with some modifications ...markers: E. coli ...
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Detection of Diarrheagenic Escherichia coli by Use of Melting Curve Analysis and Real Time Multiplex PCR
... We evaluated three enzymes: (i) Phusion (Finnzymes, Fin- land) (Phusion hot-start DNA polymerase), a Pyrococcus-like enzyme with a processivity-enhancing domain; (ii) Amplitaq gold (Applied Biosystems, Foster City, CA), ...
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Diarrheagenic Escherichia coli and Acute and Persistent Diarrhea in Returned Travelers
... The association of infection with diarrheagenic E. coli and diarrhea was analyzed by multivariate analysis with logistic re- gression. Crude ORs and results of stratified analysis to assess potential ...
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Diarrheagenic Escherichia coli pathotypes frequency in Khuzestan province of Iran
... enteroaggregative E. coli (EAEC), enterohaemorrhagic E. coli (EHEC), enteroinvasive ...E. coli (EIEC), enteropathogenic E. coli (EPEC), enterotoxigenic ...E. ...
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Type 1 Fimbriation and Its Phase Switching in Diarrheagenic Escherichia coli Strains
... Escherichia coli strains and mediate mannose-sensitive (MS) adherence to mammalian epithelial ...in E. coli is phase variable and is associated with the inversion of a short DNA element (fim ...of ...
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Prevalence and antimicrobial resistance of shiga toxin-producing Escherichia coli and enteropathogenic Escherichia coli isolated from patients with acute diarrhea
... Studies in Iran showed that DEC, such as EPEC and STEC strains, are among the most prevalent causative agents in acute diarrhea, particularly in children (15, 16). In our research blood was reported in all feces of ...
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Prevalence and antibiotic resistance patterns of diarrheagenic Escherichia coli isolated from adolescents and adults in Hamedan, Western Iran.
... for diarrheagenic E. coli was performed in patients whose stools grew ...PCRs. Diarrheagenic E. coli reference strains EIEC/ Shigella ATCC 43893 (ial + ), EPEC 2348/69 (eaeA + ), ...
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Diarrheagenic Escherichia coli and Shigella Strains Isolated from Children in a Hospital Case Control Study in Hanoi, Vietnam
... of diarrheagenic E. coli by multiplex PCR and dot blot ...individual E. coli isolates collected from the slant agars and subcultured onto MacConkey agar before PCR with single primer ...
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Prevalence of Diarrheagenic Escherichia coli in Finns with or without Diarrhea during a Round the World Trip
... of diarrheagenic Escherichia coli (enterotoxigenic, enteropathogenic, Shiga toxin-producing, and en- teroaggregative strains) by PCR from growth on primary culture ...as E. coli, and O ...
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Evaluation of Multiplex PCRs for Diagnosis of Infection with Diarrheagenic Escherichia coli and Shigella spp
... This method was then directly applied to 36 stool specimens to detect diarrheagenic E. coli and Shigella spp. These speci- mens were obtained from infants hospitalized in Hospital Sa ˜o Paulo with ...
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Detection and Characterization of Diarrheagenic Escherichia coli from Young Children in Hanoi, Vietnam
... other diarrheagenic E. coli strains, such as ETEC or EPEC strains ...of diarrheagenic E. coli in both children with diarrhea and children without ...
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Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items
... The limitations associated with traditional diagnostic techniques can be overcome by PCR which is a sensitive, specific and rapid method for diagnosis. This study showed that multiplex amplification of nucleic acid can ...
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Fecal Leukocytes in Children Infected with Diarrheagenic Escherichia coli
... Although diarrheagenic E. coli was isolated with similar fre- quencies in diarrhea and control samples, illnesses were asso- ciated with a more inflammatory ...
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Occurrence of qnr Gene in Diarrheagenic Ciprofloxacin¬-Resistant Escherichia coli Isolated from Patients with Acute Diarrhea
... Escherichia coli have been dramatically increased in last ...among Diarrheagenic E coli strains isolated from patients with acute ...43 E. coli clinical isolates recovered from ...
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Escherichia coli: a brief review of diarrheagenic pathotypes and their role in diarrheal diseases in Iran.
... of diarrheagenic E. coli has been accumulated over the years even though these complicated phenomena are not yet fully ...different diarrheagenic E. coli pathotypes in this ...
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Prevalence of E. coli O157: H7 Isolated from Patients with Acute Gastroenteritis
... 1. Xia X, Meng J, McDermott P, Ayers S, Blickenstaff K, Tran T, et al. Presence and characterization of shiga toxin producing Escherichia coli and other potentially Diarrheagenic E. coli in ...
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Virulence gene profiles and molecular genetic characteristics of diarrheagenic Escherichia coli from a hospital in western China
... DEC: diarrheagenic E. coli; EAEC: enteroaggregative ...E. coli; EHEC: enterohemor- rhagic E. coli; EPEC: enteropathogenic ...E. coli; EIEC: enteroinvasive ...
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Development of safe, effective and immunogenic vaccine candidate for diarrheagenic Escherichia coli main pathotypes in a mouse model
... main diarrheagenic E. coli pathotypes in a single vaccine using mouse ...main diarrheagenic E. coli pathotypes that is cost-effective with promise for further testing in ...
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Novel multiplex PCR for detection of diarrheagenic Escherichia coli strains isolated from stool and water samples.
... Aranda KR, Fabbricotti SH, Fagundes-Neto U and Scaletsky IC (2007). Single multiplex assay to identify simultaneously enteropathogenic, enteroaggregative, enterotoxigenic, enteroinvasive and Shiga toxin-producing ...
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<p>Multi-Drug-Resistant Diarrheagenic <em>Escherichia coli</em> Pathotypes in Pediatric Patients with Gastroenteritis from Central Iran</p>
... 2 Diarrheagenic Escherichia coli (DEC) is one of the most abundant agents in pediatric gastroenteritis, particularly in developing ...enteroaggregative E. coli (EAEC), enteropatho- genic ...
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