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Dientamoeba fragilis

Comparison and Recommendations for Use of Dientamoeba fragilis Real Time PCR Assays

Comparison and Recommendations for Use of Dientamoeba fragilis Real Time PCR Assays

... D. fragilis infections to be misdiagnosed as other gastrointestinal diseases in regions where screening for ...D. fragilis is not ...D. fragilis with trichrome stains being widely used in North ...

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Dientamoeba fragilis, One of the Neglected Intestinal Protozoa

Dientamoeba fragilis, One of the Neglected Intestinal Protozoa

... D. fragilis in their differential diagnosis of patients pre- senting with abdominal pain, diarrhea, unexplained flatulence, nausea, and ...D. fragilis infection depends on proper collection and processing ...

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Dientamoeba fragilis, a Commensal in Children in Danish Day Care Centers

Dientamoeba fragilis, a Commensal in Children in Danish Day Care Centers

... ABSTRACT Dientamoeba fragilis is an intestinal protozoan of debated clinical signif- ...D. fragilis in children aged 0 to 6 years from a 1-year multi-day-care-center cohort study set in Copenhagen, ...

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Prospective Study of the Prevalence, Genotyping, and Clinical Relevance of Dientamoeba fragilis Infections in an Australian Population

Prospective Study of the Prevalence, Genotyping, and Clinical Relevance of Dientamoeba fragilis Infections in an Australian Population

... of Dientamoeba fragilis were detected in 60 ...D. fragilis and Enterobius vermicularis, a proposed vector of transmission for ...D. fragilis. The genetic diversity of 50 D. fragilis ...

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Evaluation of Multiplex Tandem Real Time PCR for Detection of Cryptosporidium spp , Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in Clinical Stool Samples

Evaluation of Multiplex Tandem Real Time PCR for Detection of Cryptosporidium spp , Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in Clinical Stool Samples

... spp., Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis, from human clinical ...D. fragilis, 11 ...D. fragilis, 47% and 97% for ...

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Study of Dientamoeba Fragilis by Iron Hematoxylin Staining and Nested-PCR Methods in Chalous Healthcare Refers

Study of Dientamoeba Fragilis by Iron Hematoxylin Staining and Nested-PCR Methods in Chalous Healthcare Refers

... 14- Stark D, Beebe N, Marriott D, Ellis J, Harkness J. Prospective study of the prevalence, genotyping, and clinical relevance of Dientamoeba fragilis infections in an Australian population. J Clin ...

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Direct Amplification and Genotyping of Dientamoeba fragilis from Human Stool Specimens

Direct Amplification and Genotyping of Dientamoeba fragilis from Human Stool Specimens

... microscopy-detected Dientamoeba fragilis was amplified by PCR with the primers and conditions de- scribed by Johnson and Clark ...D. fragilis (kindly provided by ...D. fragilis (14) was not ...

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Additional Glance on the Role of Dientamoeba fragilis &  Blastocystis hominis in Patients with Irritable Bowel Syndrome

Additional Glance on the Role of Dientamoeba fragilis & Blastocystis hominis in Patients with Irritable Bowel Syndrome

... Dientamoeba fragilis is a human intestinal pro- tozoan parasite, commonly reported world- wide and associated with gastrointestinal man- ifestations ...(6). Dientamoeba remains neglected despite many ...

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MALDI TOF MS as a new tool for the identification of Dientamoeba fragilis

MALDI TOF MS as a new tool for the identification of Dientamoeba fragilis

... D. fragilis is posi- tively influenced by the examination of multiple fecal samples, the use of suitable staining techniques and the use of culture, which has proven to be two times more sensitive than stained ...

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The returned traveller with diarrhoea

The returned traveller with diarrhoea

... paratyphi Presents initially with constipation but may develop diarrhoea later in infection Protozoa Dientamoeba fragilis Requires permanent faecal stained smear Blastocystis hominis If [r] ...

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Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil

Molecular characterization of intestinal protozoa in two poor communities in the State of São Paulo, Brazil

... The DNAs extracted from water samples were also tested by PCR for Blastocystis sp., and amplification was observed from 3 samples collected in PS. Direct sequen- cing of PCR products generated overlapping chromato- ...

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Vaginal carriage of enterotoxigenic Bacteroides fragilis in pregnant women

Vaginal carriage of enterotoxigenic Bacteroides fragilis in pregnant women

... B. fragilis are sparse: the most recent review on the subject, dating back to 1992, proclaims that besides serological assays, exploration of the newer molecular biology-based techniques is mandatory ...

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Detection of Bacteroides fragilis in clinical specimens by PCR

Detection of Bacteroides fragilis in clinical specimens by PCR

... Four specimens that were positive by culture two samples of bronchial aspirate from one patient with pneumonia, one sample of pleural fluid from a patient with pyothorax, and one sample [r] ...

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Use of an immunoperoxidase method for identification of Bacteroides fragilis

Use of an immunoperoxidase method for identification of Bacteroides fragilis

... These strains included three reference strains ATCC 23745, VPI 2553, and 2393, three CDC strains, and five isolates from anaerobic infections obtained from the University Hospital labora[r] ...

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Immunochemical evidence for multiple serotypes of Bacteroides fragilis

Immunochemical evidence for multiple serotypes of Bacteroides fragilis

... When the outer membrane fractions were compared by double diffusion in gel against antiserum CDC 14462 live cells, two strong precipitin lines formed with its homologous antigen and one [r] ...

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Detection of enterotoxigenic Bacteroides fragilis in patients with ulcerative colitis

Detection of enterotoxigenic Bacteroides fragilis in patients with ulcerative colitis

... Therefore, different methods like real time PCR can be used for detection and quantification of these organisms. The rate of B. fragilis harboring bft gene was higher in UC population in comparison with nIBD, as ...

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Early intestinal Bacteroides fragilis colonisation and development of asthma

Early intestinal Bacteroides fragilis colonisation and development of asthma

... Modulation of the composition of the gut flora could con- tribute to alleviation of the symptoms of asthma. The desirable modification was identified as restraining of the Bacteroides fragilis flora. Detailed ...

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Superoxide dismutase in Bacteroides fragilis and related Bacteroides species

Superoxide dismutase in Bacteroides fragilis and related Bacteroides species

... Superoxide dismutase SOD activity was demonstrated in cell-free extracts of Bacteroides fragilis, Bacteroides vulgatus, Bacteroides distasonis, Bacteroides ovatus, and Bacteroides thetai[r] ...

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Rapid identification of Bacteroides fragilis with bile and antibiotic disks

Rapid identification of Bacteroides fragilis with bile and antibiotic disks

... fragilis if it: i grows rapidly in fresh thioglycolate broth, ii provides a heavy lawn of growth on susceptibility test plates within 24 h, iii is resistant to disks containing 1,000 ,g [r] ...

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Serological study of trichloroacetic acid extracts of Bacteroides fragilis

Serological study of trichloroacetic acid extracts of Bacteroides fragilis

... In three cross-reactions, precipitates intersected with the autologous precipitin lines instead of coalescing in arcs; hence, lines of nonidentity formed in some tests e.g., antisera 121[r] ...

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