Diphtheria Toxin
Heterogeneity of diphtheria toxin gene, tox, and its regulatory element, dtxR, in Corynebacterium diphtheriae strains causing epidemic diphtheria in Russia and Ukraine
... of diphtheria toxin with the severity of the clinical presentation of patients with diphtheria are under way and may allow for a deeper understanding of this regulatory mechanism and of an ...
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PG13 Packaging Cells Produce Recombinant Retroviruses Carrying a Diphtheria Toxin Mutant Which Kills Cancer Cells
... Suicide gene therapy has been widely investigated for the treatment of human immunodeficiency virus infection (2), for controlling graft-versus-host disease (31), and also for the treatment of cancer (2). Suicide genes ...
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Dmp1 Promoter-Driven Diphtheria Toxin Receptor Transgene Expression Directs Unforeseen Effects in Multiple Tissues
... human diphtheria toxin receptor (the heparin-binding epidermal growth factor; HDTR) driven by a 10-kb dentin matrix protein (Dmp1) promoter fragment in order to facilitate selective osteocyte ablation upon ...
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Next generation sequencing analysis of nine Corynebacterium ulcerans isolates reveals zoonotic transmission and a novel putative diphtheria toxin-encoding pathogenicity island
... by diphtheria toxin (DT)-produ- cing strains of the three Corynebacterium species, ...of diphtheria, which is characterized by a greyish pseudomembrane at the in- fection site both in respiratory or ...
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Development of targeted therapy for bladder cancer mediated by a double promoter plasmid expressing diphtheria toxin under the control of H19 and IGF2 P4 regulatory sequences
... The H19-Luc plasmid which contains the luciferase gene under the control of the human H19 promoter region from nucleotide -818 to + 14 was prepared as described [28]. The H19-Luc plasmid was digested with XbaI and NcoI, ...
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Molecular Characterization of Diphtheria Toxin Repressor (dtxR) Genes Present in Nontoxigenic Corynebacterium diphtheriae Strains Isolated in the United Kingdom
... of diphtheria toxin by toxigenic strains of Corynebacterium diphtheriae is regulated in an iron-dependent manner by the diphtheria toxin repressor protein (DtxR) (13, ...of diphtheria ...
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Diphtheria toxin–mediated ablation of lymphatic endothelial cells results in progressive lymphedema
... Development of novel treatments for lymphedema has been limited by the fact that the pathophysiology of this disease is poorly understood. It remains unknown, for example, why limb swelling resulting from surgical injury ...
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Measuring Immunoglobulin G Antibodies to Tetanus Toxin, Diphtheria Toxin, and Pertussis Toxin with Single-Antigen Enzyme-Linked Immunosorbent Assays and a Bead-Based Multiplex Assay
... tetanus toxin, diphtheria toxin, and pertussis toxin (PT) from Bordetella ...tetanus toxin, and diphtheria toxin were measured in 123 serum pairs (total of 246 sera) from ...
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Effect of the T-domain on intracellular transport of diphtheria toxin
... That is why further detailed study of mecha - nisms of SubA translocation into cytosol and mecha - nisms of resistance to DT needs new experimental models and new instruments for research of these processes. One of such ...
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Analyzing Temperature Dependence of Diphtheria Toxin Structure and Stability Using Molecular Dynamics Simulations
... The protein denaturation due to the temperature variation and the free energy difference provided useful information on the protein stability. This study was performed to evaluation of the thermodynamic stability of ...
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Comparison of Phenotypic and Genotypic Methods for Detection of Diphtheria Toxin among Isolates of Pathogenic Corynebacteria
... of diphtheria toxin from 55 isolates of Corynebacterium diphtheriae and Corynebacterium ulcerans originally isolated in five different ...of diphtheria were compared and evaluated against the “gold ...
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Development of a Real Time Fluorescence PCR Assay for Rapid Detection of the Diphtheria Toxin Gene
... of diphtheria cases is currently de- pendent on culturing Corynebacterium diphtheriae and per- forming the Elek test (2) to determine the organism’s toxige- ...the diphtheria toxin gene, tox, is ...
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Mycobacterium bovis BCG as a Delivery System for the dtb Gene Antigen from Diphtheria Toxin
... Diphtheria is a fulminant bacterial disease caused by toxigenic strains of Co- rynebacterium diphtheriae whose local and systemic manifestations are due to the action of the diphtheria toxin (DT). ...
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Treatment of ovarian cancer ascites by intra peritoneal injection of diphtheria toxin A chain H19 vector: a case report
... Diphtheria toxin is secreted from Corynebacterium diphtheriae as a single polypeptide chain containing two major domains: A chain (amino-terminal, 193 residues), which carries the active site for adenosine ...
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Enhancement of internalization of diphtheria toxin recombinant fragments in sensitive cells mediated by toxin’s T-domain
... of diphtheria toxin (DT) and its R-domain differ by the presence of ...these toxin fragments with mammalian cells in order to evaluate the T-domain’s influence on endocytosis in resistant ...in ...
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Metal Ion Binding of The Corynebacterium pseudotuberculosis Diphtheria Toxin Repressor
... The acquisition of iron is essential to facilitate growth of bacterial pathogens. Bacteria using high-affinity iron uptake systems, siderophores, to scavenge iron from the host is regulated by the diphtheria ...
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A new mouse model for renal lesions produced by intravenous injection of diphtheria toxin A-chain expression plasmid
... In this study, we intended to produce a new mouse model of renal failure using a simple and convenient method through in vivo introduction of non-viral DNA carrying a toxic gene such a diphtheria toxin ...
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The cytotoxic effect of diphtheria toxin on the actin cytoskeleton
... Abstract: Diphtheria toxin (DT) and its N-terminal fragment A (FA) catalyse the transfer of the ADP-ribose moiety of nicotinamide adenine dinucleotide (NAD) into a covalent linkage with eukaryotic ...
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Identification of Formaldehyde-Induced Modifications in Diphtheria Toxin
... in diphtheria toxoid by using stable isotope labeling ...in diphtheria toxin after reductive dimethylation and digestion was determined by LC-MS/MS and database search ...in diphtheria toxoid ...
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Production and Purification of Polyclonal Antibodies against Diphtheria Toxin
... This toxin consists of two chains, catalytic chain (A) and binding (B) ...the toxin binds to its receptor on numerous body cells such as myocardial, kidney and peripheral nerve ...of diphtheria ...
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