Direct fluorescent antibody
Comparison of PCR, Culture, and Direct Fluorescent Antibody Testing for Detection of Bordetella pertussis
... culture, direct fluorescent-antibody testing (DFA), and an in-house-developed PCR test targeting the repeated insertion sequence IS481 for the detection of Bordetella pertussis in nasopharyngeal swab ...
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Comparison of Automated Quantitative Reverse Transcription PCR and Direct Fluorescent Antibody Detection for Routine Rabies Diagnosis in the United States
... The direct-fluorescent antibody test (DFA) (4) is a rapid and sensitive method for diag- nosing rabies infection, and as a World Organisation for Animal Health (OIE)/World Health Organization ...
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Retrospective examination of lung tissue specimens for the presence of Legionella organisms: comparison of an indirect fluorescent antibody system with direct fluorescent antibody testing
... The working dilution of the goat anti-rabbit immunoglobulin fluorescein conjugate was used to titrate each of the rabbit antisera for the various Legionella strains.. The antisera to the[r] ...
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Optimal fluorescein to protein ratios of bacterial direct fluorescent antibody reagents
... For all systems studied, a fluorescein-to-protein ratio of 30 was optimal for conjugates prepared from ammonium sulfate fractions >75% gamma globulin and pure immunoglobulin G desorbed f[r] ...
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Correlation of an expanded direct fluorescent antibody system with an established passive hemagglutination system for serogrouping strains of Campylobacter jejuni and Campylobacter coli
... Direct FA staining reactions of the 33 FA immunogens 2 and 8, 3 and 5, 4 and 10, and 6 strains from frequently encountered passive HA and 15 had distinctly different reactions when serot[r] ...
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Determination of antigenic relationships among legionellae and non legionellae by direct fluorescent antibody and immunodiffusion tests
... In the ID test, serogroup 3 antiserum in the center well gave strong precipitin lines with Bloomington 2 urea and autoclaved extracts, whereas ABB50 urea extract gave weak, diffuse preci[r] ...
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Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture
... We developed a rapid thermocycling, real-time detection (also known as real-time PCR) method for the detection of Legionella species directly from clinical specimens. This method uses the LightCycler (Roche Molecular ...
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Comparison of Methods for Detection of Vaccinia Virus in Patient Specimens
... We analyzed a shell vial culture assay (SVA), real-time PCR, and a direct fluorescent antibody assay (DFA) for rapid detection of vaccinia virus from vaccination sites of Dryvax vaccine recipients. ...
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SOFIA®RSV: prospective laboratory evaluation and implementation of a rapid diagnostic test in a pediatric emergency ward
... DFA: Direct fluorescent antibody; ED: Emergency department; FluA: Influenza virus type A; FluB: Influenza virus type B; hAdV: Human adenovirus; hCoV: Human coronaviruses; hMPV: Human metapneumovirus; ...
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Evaluation of five methods for respiratory syncytial virus detection
... A total of 117 nasal aspirates were cultured for respiratory syncytial virus RSV and tested for RSV antigen by a direct fluorescent-antibody DFA test Bartels Immunodiagnostic Supplies, I[r] ...
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Multiplex LightCycler PCR Assay for Detection and Differentiation of Bordetella pertussis and Bordetella parapertussis in Nasopharyngeal Specimens
... The wide prevalence of pertussis and its changing epidemi- ology have highlighted the need for rapid and sensitive meth- ods for diagnosing Bordetella pertussis infection. Laboratory criteria for the diagnosis of ...
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Development and testing of monoclonal antibody based rapid immunodiagnostic test kits for direct detection of Vibrio cholerae O139 synonym Bengal
... Screen, a coagglutination test (6); Cholera SMART, a colloidal gold-based colorimetric test (10); and Cholera DFA, a direct fluorescent-antibody (DFA) test (9), proved highly specific and sensitive ...
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Comparison of the Phadebact Gonococcus Test with the rapid fermentation method
... There are three generally accepted methods currently employed in laboratories for identification of Neisseria species: the direct fluorescent-antibody technique, carbohydrate degradation[r] ...
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Comparison of rabies humoral antibody titers in rabbits and humans by indirect radioimmunoassay, rapid fluorescent focus inhibition technique, and indirect fluorescent antibody assay
... i Like IFA tests, the RIA test measured antibodies induced by the RNP antigen; ii the RIA test results are similar to the IFA results because the viral antigens used were cell-associated[r] ...
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Detection of anti-Borrelia burgdorferi antibody responses with the borreliacidal antibody test, indirect fluorescent-antibody assay performed by flow cytometry, and western immunoblotting.
... Briefly, samples were boiled in sample buffer for 5 min, and 150 mg of total protein was loaded onto a 12% polyacrylamide reducing gel (4% polyacrylamide stacking gel with comb). Two wells on either side of the gel ...
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Baculovirus Surface Display Using Infuenza Neuraminidase (NA) Transmembrane Anchor
... To verify the expression of NA-AngII in insect cells, total RNA from infected Sf-9 cells was extracted by TRIZOL reagent (Invitrogen, USA) and cDNA was prepared according to manufacture protocol (Thermo scientific, USA). ...
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Fluorescent treponemal antibody absorption double staining procedure
... RESULTS The Ortholux II microscope equipped with a N filter cube for reading rhodamine fluorescence was used in establishing acceptability of the proposed FTA-ABS DS reference reagents..[r] ...
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Comparison of fluorescent antibody, neutralizing antibody, and complement enhanced neutralizing antibody assays for detection of serum antibody to respiratory syncytial virus
... A comparison of three assays for the detection of serum antibody to respiratory syncytial virus RSV was carried out on 47 serum samples obtained sequentially from infants and young child[r] ...
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Puerperal Bacteremia and Neonatal Sepsis Due to Hemophilus Parainfluenzae: Report of a Case with Antibody Titers
... Puerperal bacteremia and neonatal sepsis due to Haemophilus parainfluenzae is described in a mother and her infant. Indirect fluorescent antibody studies for specific 1gM antibody di- re[r] ...
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Immunoglobulin specificity for the fluorescent treponemal antibody absorption test conjugate
... The fluorescent treponemal antibody-absorption FTA-ABS test is currently recommended as a confirnatory test for patients whose sera react in reagin tests for syphilis 7.. When the test i[r] ...
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