direct PCR
Human malaria diagnosis using a single step direct PCR based on the Plasmodium cytochrome oxidase III gene
... COX-III direct PCR (limit of detection: ...18s-rRNA direct nested PCR (limit of detection: 2–10 ...COX-III direct PCR identified all 21 positive controls (no mixed infections ...
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Development of a direct PCR assay for detection of the diphtheria toxin gene
... the direct PCR is overcoming the effect of numerous inhibitory factors that may be present in the clinical sample and the swab ...the PCR inhibitor and that a step that removes the proteinase K would ...
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Methicillin Resistant Staphylococcus aureus (MRSA) Detection: Comparison of Two Molecular Methods (IDI MRSA PCR Assay and GenoType MRSA Direct PCR Assay) with Three Selective MRSA Agars (MRSA ID, MRSASelect, and CHROMagar MRSA) for Use with Infection Control Swabs
... (IDI-MRSA PCR assay and GenoType MRSA Direct PCR assay) and three selective MRSA agars (MRSA ID, MRSASelect, and CHROMagar MRSA), using 205 (101 nasal, 52 groin, and 52 axillary samples) samples from ...
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Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections
... pneumoniae in respiratory tract samples, although future studies must be performed to evaluate the correlation between a positive PCR result and clinical symptoms.. These investigations [r] ...
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Direct PCR analysis for toxigenic Pasteurella multocida
... the PCR assay avoids potential problems of suppressed gene ex- ...A PCR-based assay theoretically could give a positive signal from an isolate with a mutated, nonfunctional gene; however, there is no ...
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Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk
... multiplex PCR, the use combination primers for the detection of several genes eliminates the possibility of false positives, which may occur if non-O157:H7 strains were to acquire an O157:H7 specific ...(21). ...
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Ultrasensitive Amplification Refractory Mutation System Real Time PCR (ARMS RT PCR) Assay for Detection of Minority Hepatitis B Virus Resistant Strains in the Era of Personalized Medicine
... using direct PCR Sanger sequencing. The ARMS RT-PCR with molecular beacon assay showed a sensitivity of 100% for the detection of mutant variants A181V, T184A, and ...by direct PCR ...
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Direct detection of verotoxin producing Escherichia coli in stool samples by PCR
... plates, direct PCR of stool samples, colony blot testing of stool samples, PCR of the isolates recovered from the stool samples, in vitro toxin production by the isolates recovered from stool ...
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Human Cytomegalovirus: detection of congenital and perinatal infection in Argentina
... infections, direct PCR in urine was positive in a high percentage (73%) of samples; however, in patients grouped as with perinatal infection only 36% of the cases were ...97%. PCR technique performed ...
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Direct Detection of eae Positive Bacteria in Human and Veterinary Colorectal Specimens by PCR
... this PCR would not be restricted to the eae gene of these bac- terial species, meaning that it could have detected additional eae-positive ...pathogens. Direct PCR may also provide an initial general ...
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Comparison of Simplexa HSV 1 & 2 PCR with Culture, Immunofluorescence, and Laboratory Developed TaqMan PCR for Detection of Herpes Simplex Virus in Swab Specimens
... laboratory-developed PCR than for culture but labor costs are less (4, ...Simplexa PCR is significantly more expensive than LDT PCR, it offers con- venience, HSV typing in the initial screen, and an ...
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Direct identification and typing of Mycobacterium tuberculosis by PCR
... entire PCR fingerprinting assay can be converted to a comput- er-automated system simply by synthesizing either PCR primer with a fluorescent label and running the assay samples in the Applied Biosystems ...
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Bartonella vinsonii subsp berkhoffii and Bartonella henselae bacteremia in a father and daughter with neurological disease
... Based upon prior detection of B. vinsonii subsp. ber- khoffii in the father’s blood sample, a decision was made to test the daughter. The daughter was seroreactive to antigens of B. vinsonii subsp. berkhoffii genotypes ...
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A simple, flexible and efficient PCR-fusion/Gateway cloning procedure for gene fusion, site-directed mutagenesis, short sequence insertion and domain deletions and swaps
... Overlapping PCR involves PCR amplification of selected DNA regions with complementary primers generating DNA fragments with overlapping ends ...by PCR amplification of the entire assembled DNA ...by ...
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Accuracy of real time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
... CSF Gram staining, long considered a mainstay of bac- terial meningitis diagnosis [1], is rapid, inexpensive, and requires relatively little training. However, accurate results are highly dependent on the operator’s ...
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Molecular diagnosis of bacterial endocarditis by broad range PCR amplification and direct sequencing
... DNA was extracted from the tissues as previously described (10, 11). Broad-spectrum amplification, hybridization, and sequencing. For each spec- imen, two amounts of crude DNA extract (2 and 0.2 m l) were directly ...
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Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens
... Preparation of samples for PCR analysis. All reagents and equipment for performance of the Amplicor PCR assay were supplied by RMS. This test was performed according to draft package insert instructions for ...
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IDH1 mutation detection by droplet digital PCR in glioma
... Digital PCR is an end-point method that quantifies nucleic acids without standard curves and is independent of reaction ...digital PCR, which is the same as that of qRT-PCR, is specific amplification ...
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Economic Evaluation of Laboratory Testing Strategies for Hospital Associated Clostridium difficile Infection
... include direct tissue culture cytotox- icity, plate reader enzyme immunoassay (EIA) for ...batch PCR for genes in the pathogenicity locus ...demand PCR (odPCR) (GeneXpert; Cepheid, ...
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Evaluation of Gen Probe Amplified Mycobacterium Tuberculosis Direct Test and PCR for direct detection of Mycobacterium tuberculosis in clinical specimens
... The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test AMTD is a direct specimen assay for the identification of Mycobacterium tuberculosis from respiratory samples.. rRNA is amp[r] ...
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