Direct Tissue Profiling and Peptide identification
Identification of potential serum peptide biomarkers of biliary tract cancer using MALDI MS profiling.
... Detection of MALDI-TOF peaks discriminating BTC and healthy controls Ninety two serum samples comprising patients with BTC (n = 39), benign biliary disease (n = 31), and healthy con- trols (n = 22) were subjected to C18 ...
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Injectable Peptide Decorated Functional Nanofibrous Hollow Microspheres to Direct Stem Cell Differentiation and Tissue Regeneration
... the peptide-decorated FNF- HMS could induce the aimed tissue ...mimicking peptide (P24) was conjugated onto the FNF-HMS and was shown to enhance BMSC osteogenesis and bone ...
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Profiling Sortase Substrate Specificity using Peptide Libraries
... substrate profiling data described in Chapter 2 of this thesis, we hypothesized that one or more of our sortase homologs would be able to process this substrate, and therefore provide a direct route to ...
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Identification of the agr Peptide of Listeria monocytogenes
... Despite similarities on protein level, genetic organization, and phenotypic traits regulated, known agr systems differ regarding their mechanisms of target gene regulation. While in staphylococci, a significant number of ...
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Methods for peptide identification by spectral comparison
... that peptide and instrument-specific factors play a significant role in the direct spectral ...the peptide associated with the reference spectrum or ...
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Evaluation of Peptide Nucleic Acid Fluorescence In Situ Hybridization for Identification of Clinically Relevant Mycobacteria in Clinical Specimens and Tissue Sections
... procedure was established and evaluated by using mycobacte- ria cultured from clinical specimens. M. tuberculosis complex-, M. leprae-, and M. avium-specific PNA probes were used suc- cessfully for specific visualization ...
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Fusarium proliferatum Soft Tissue Infection at the Site of a Puncture by a Plant: Recovery, Isolation, and Direct Molecular Identification
... The environmental ubiquity of Fusarium spp. and their as- sociation with plants coincide with the cutaneous inoculation from the plant spine in this patient’s history. Prior to the development of neutropenia, his ...
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Screening and Identification of a Targeting Peptide to Hepatocarcinoma from a Phage Display Peptide Library
... playing peptide of the phage S1, HCBP1, was also able to maintain binding speci- ficity to hepatoma cells rather than nor- mal liver cells in vitro, also proving that its binding ability was determined by the ...
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Section 6 Hazard Identification, Profiling, and Ranking
... Page 6-32 The major elements of the Passaic River “Main Stem” flood damage reduction project consist of two underground tunnels, a 20.4 mile-long main tunnel 42-foot diameter and a 1.3 mile-long 23-foot diameter spur ...
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Screening and identification of a renal carcinoma specific peptide from a phage display peptide library
... To confirm the binding ability of the selected phage toward target A498 cells, the phage clone M13 (clone ZT-2) was isolated, amplified and purified for immuno- chemical assay. The HK-2 cell line, composed of human ...
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Systematic profiling of spatiotemporal tissue and cellular stiffness in the developing brain
... have direct effects on fate determination in several cell types in ...developing tissue using the mouse embryonic cerebral cortex as an experimental ...of tissue and cellular stiffness with ...
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Transcriptional profiling and biomarker identification reveal tissue specific effects of expanded ataxin-3 in a spinocerebellar ataxia type 3 mouse model
... Results: Despite normal motor performance at 17.5 months of age, transcriptional changes in brain tissue of the SCA3 mice were observed. Most transcriptional changes occurred in brainstem and striatum, whilst ...
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Proteolytic Profiling with Imaging Agents for Rational Design of Target-Activated Peptide Prodrugs.
... 3.2 Introduction To date, many researchers have focused on developing prodrugs to improve the bioavailability of poorly absorbed drugs. Often these prodrugs are activated prior to reaching systemic circulation and so ...
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Peptide profiling by capillary separation techniques coupled to mass spectrometry Gaspari, Marco
... Dynamic range of detection of most mass spectrometers is not sufficient to tackle global proteomic investigations. Thus, improvements in this field will be of special interest. From the qualitative analysis side, tandem ...
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Identification of a novel lytic peptide for the treatment of solid tumours
... The biodistribution of 125 I-labelled Cypep-1 showed a rapid initial plasma clearance, as may be expected due to the fast distribution within the extracellular space of a small 27 amino acids molecule (Fig. 5A). Its ...
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Glycomic Profiling of Tissue Sections by LC-MS
... In order to determine the enzyme concentration needed for efficient glycan release, RNase B and fetuin were subjected to enzymatic digestion at different concentrations of PNGase F.. T[r] ...
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Protease Peptide Microarrays Ready-to-use microarrays for protease profiling
... Protease Peptide Microarray is designed as a ready-to-use product to assay enzyme activity directly on the surface of the glass ...cleaving peptide bonds within the protein used for blocking! Protease ...
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Peptide growth factors and inflammation, tissue repair, and cancer
... Although many peptide growth factors will eventually be found to exert an autocrine action in cancer cells, current re- search has focused on three principal autocrine peptides, namely, [r] ...
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Characterization and corneal tissue engineering application of peptide amphiphiles
... 52 ECM and stroma interaction forms the key in cell based regenerative medicine approach for corneal tissue engineering. Stroma consists of acellular extracellular matrix environment formed by parallel aligned ...
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Antibody-based tissue profiling as a tool for clinical proteomics
... defined tissue, for example, islet cells in pancreatic tissue or glomerulus cells in kidney ...crude tissue core as ...normal tissue, for example, for cerebellum Purkinje cells, cells of ...
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