Disulfide isomerase
Intracellular Trafficking and Glycobiology of TbPDI2, a Stage-Specific Protein Disulfide Isomerase in Trypanosoma brucei
... Trypanosoma brucei protein disulfide isomerase 2 (TbPDI2) is a bloodstream stage-specific lumenal endoplasmic reticulum (ER) glycoprotein. ER localization is dependent on the TbPDI2 C-terminal tetrapeptide ...
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High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli
... were selected in our study (Additional file 2: Table S2 Data). In every case a hexa-histidine (6HIS) tag was introduced to enable the downstream purification of the fusion proteins using immobilized-nickel affinity chro- ...
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The C terminal CGHC motif of protein disulfide isomerase supports thrombosis
... Protein disulfide isomerase (PDI) is the prototypic member of the PDI family of enzymes that catalyze the reversible formation and isomerization of disulfide bonds ...
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Blastomyces Virulence Adhesin 1 Protein Binding to Glycosaminoglycans Is Enhanced by Protein Disulfide Isomerase
... of disulfide bond reduction in heparin-specific binding by ...protein disulfide isomerase (PDI) enhances binding to heparin on solid surfaces, (iii) that inhibition of PDI impairs binding mediated by ...
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Cell surface protein disulfide isomerase catalyzes transnitrosation and regulates intracellular transfer of nitric oxide
... Since thiols can undergo nitrosation and the cell membrane is rich in thiol-containing proteins, we con- sidered the possibility that membrane surface thiols may regulate cellular entry of NO. Recently, protein ...
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Downregulation of protein disulfide isomerase in sepsis and its role in tumor necrosis factor alpha release
... Protein disulfide isomerase (PDI) catalyses the formation, breakage, and rearrangement of disulfide bonds within a mol- ...[21]. Disulfide forma- tion involves the endogenous oxidized and ...
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Lemon protein disulfide isomerase: cDNA cloning and biochemical characterization
... Protein disulfide isomerase (PDI), a member of thioredoxin- superfamily, plays a key role in catalyzing disulfide bond for- mation, reduction, and ...native disulfide bond formation involves ...
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Expression of protein disulfide isomerase A3 precursor in colorectal cancer
... Figure 5 Effect of protein disulfide isomerase A3 precursor (PDIA3) knockdown on apoptosis. (A) SW480 cells were treated with small interfering RNA (siRNA)-PDIA3, and then the cells were stained with TUNEL ...
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Altered Oxido-Reductive State in the Diabetic Heart: Loss of Cardioprotection due to Protein Disulfide Isomerase
... Diabetes is associated with an increased risk of heart failure, in part explained by endoplasmic reticulum stress and apoptosis. Protein disulfide isomerase (PDI) prevents stressed cardiomyocytes apoptosis. ...
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A critical role for extracellular protein disulfide isomerase during thrombus formation in mice
... protein disulfide isomerase (PDI), catalyze disulfide oxidation, reduction, and isomerization, thereby playing an important role in protein ...
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Identification and characterization of the protein disulfide isomerase multigene family in plants.
... Protein Disulfide Isomerase Multigene Family in ...Protein disulfide isomerases (PDIs) contain thioredoxin domains and aid in the formation of proper disulfide bonds during protein ...
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Inhibition of protein disulfide isomerase in glioblastoma causes marked downregulation of DNA repair and DNA damage response genes
... Overexpression of protein disulfide isomerase (PDI) promotes cancer growth, suggesting that it represents a potential cancer therapeutic target [8-10]. PDI is a dithiol-disulfide oxidoreductase with ...
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Investigations into the Regulation of the Redox Activity of Protein Disulfide Isomerase by Active Site-Flanking Lysines
... protein disulfide isomerase (PDI: EC ...of disulfide bonds on multiple proteins (Goldberger, Epstein, & Anfinsen, ...isomerize disulfide bonds as well as oxidize thiols (Figure ...
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‘Something in the way she moves’ : the functional significance of flexibility in the multiple roles of protein disulfide isomerase (PDI)
... ‘native’ disulfide bonds (Goldberger et al, 1963; Venetianer & Straub, ...protein disulfide isomerase (PDI) – was first used in 1975 (Hawkins & Freedman, 1975), and initially, evidence on its ...
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The branched chain aminotransferase proteins: Novel redox chaperones for protein disulfide isomerase implications in Alzheimer's disease
... poor isomerase activity, suggesting that a second CXXC motif is required for maximal dithiol- disulfide isomerase activity ...of disulfide bonds, their isomerase activity is ...
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Protein disulfide isomerase inhibitors constitute a new class of antithrombotic agents
... Thrombosis, or blood clot formation, and its sequelae remain a leading cause of morbidity and mortality, and recurrent thrombosis is common despite current optimal therapy. Protein disulfide isomerase (PDI) ...
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Endothelial cells microparticle-associated protein disulfide isomerase promotes platelet activation in metabolic syndrome
... Materials and Methods: We obtained plasma from 23 MetS patients and 8 normal healthy controls. First we built insulin resistance (IR) model of human umbilical vein endothelial cells (HUVECs), and then we separated EMPs ...
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Binding of Host Cell Surface Protein Disulfide Isomerase by Anaplasma phagocytophilum Asp14 Enables Pathogen Infection
... ABSTRACT Diverse intracellular pathogens rely on eukaryotic cell surface disulfide reductases to invade host cells. Pharmacologic inhibition of these enzymes is cyto- toxic, making it impractical for treatment. ...
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Protein disulfide isomerase interacts with a substrate protein at all stages along its folding pathway
... 1. Disulfide generation pathway for oxidative folding of reduced ...the disulfide bonds that are present with colour intensity reflecting the extent of native ...non-native disulfide and occur ...
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Protein disulfide isomerase inhibition blocks thrombin generation in humans by interfering with platelet factor V activation
... The precise mechanism by which PDI influences the generation of platelet-dependent factor Va has yet to be elucidated. Factor V contains 18 cysteine residues: 14 form intramolecular disulfide bridges and 4 are ...
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