DNA and molecular cloning
HCS Exercise 1 Dr. Jones Spring Recombinant DNA (Molecular Cloning) exercise:
... Recombinant DNA (Molecular Cloning) exercise: The purpose of this exercise is to learn techniques used to create recombinant DNA or clone ...
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Molecular cloning of unintegrated and a portion of integrated moloney murine leukemia viral DNA in bacteriophage lambda.
... Molecular cloning of infectious integrated murine leukemia virus DNA from infected mouse cells. Nucleotide sequence of the rightward operator of phage A[r] ...
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Molecular cloning and physical mapping of murine cytomegalovirus DNA.
... The results obtained after hybridiza- tion of 32P-labeled cloned fragments to Southern blots of MCMV DNA cleaved with HindIII, XbaI, EcoRI, BamHI, ApaI, ClaI, EcoRV, or KpnI allowed us t[r] ...
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Molecular cloning of covalently closed circular DNA of bovine leukemia virus.
... Comprehensive restriction endonuclease mapping of the unintegrated bovine leukemia virus and the cloned DNA molecules showed that eight of the nine clones carried viral information witho[r] ...
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Molecular cloning and characterization of avian sarcoma virus circular DNA molecules.
... Thus, digestion of supercoiled SR-A DNA by SacI results in permuted full- length linear molecules that contain either one or two copies of the terminal redundancy located near the right [r] ...
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Molecular cloning of a family of retroviral sequences found in chimpanzee but not human DNA.
... Thus the human sequences detected with 5CH3 are more closely related to murine retro- viruses than they are to the CPC-1 family of primate retroviruses. ACKNOWLEDGMENTS[r] ...
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Ground squirrel hepatitis virus DNA: molecular cloning and comparison with hepatitis B virus DNA.
... sequence of the gene coding for the major protein of hepatitis B virus surface antigen. Serological relationship of woodchuck hep- atitis virus and human hepatitis B virus.[r] ...
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Rauscher murine leukemia virus: molecular cloning of infectious integrated proviral DNA.
... The molecularly cloned Rauscher murine leukemia virus should be useful in studying the molecular mechanisms involved in the transformation of specific lymphoid target cells by chronic mo[r] ...
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Virion DNA of ground squirrel hepatitis virus: structural analysis and molecular cloning.
... The structure of the encapsidated DNA genome of ground squirrel hepatitis virus (GSHV) has been examined by restriction endonuclease cleavage, nucleic acid hybridization, and molecular c[r] ...
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Molecular cloning and characterization of murine leukemia virus-related DNA sequences from C3H/HeN mouse DNA.
... Comparison of the restriction endonu- clease cleavage maps of these C3H clones with maps recently developed for ecotropic and xenotropic MuLV DNAs indicates that C3H clone 14-9 correspon[r] ...
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Fine mapping and molecular cloning of mutations in the herpes simplex virus DNA polymerase locus.
... Mutations in five phenotypically distinct mutants derived from herpes simplex virus type 1 strain KOS which lie in or near the herpes simplex virus DNA polymerase (pol) locus have been f[r] ...
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Molecular cloning of infectious viral DNA from ecotropic neurotropic wild mouse retrovirus.
... Wild mouse ecotropic murine leukemia virus infection of inbred mice: dual- tropic virus expression precedes the onset of paralysis and lymphoma. Characterization of genome struc- ture of[r] ...
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FBJ murine osteosarcoma virus: identification and molecular cloning of biologically active proviral DNA.
... complex consists of a replication-competent helper murine leukemia virus (FBJ-MLV) and a replication-defective transforming murine sar-.. coma virus (FBJ-MSV) (23, 24).[r] ...
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Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma.
... Hybridiza- tion with the DNAs of human papilloma virus types 1, 2, 3, 4, 5, and 8 showed no homology under stringent hybridization conditions.. Human papilloma virus type 6 DNA, however,[r] ...
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Molecular cloning and chromosomal mapping of DNA rearranged with the parathyroid hormone gene in a parathyroid adenoma
... a DNA rearrangement occurred at the PTH locus; (b) the rearrangement separated the PTH gene's 5' flanking region from its coding exons, conceivably placing a newly adjacent gene under the influence of PTH ...
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Molecular cloning of proviral DNA and structural analysis of the transduced myc oncogene of avian oncovirus CMII.
... cloning of CMII proviral DNA, we compare its genetic structure and particularly its transforming gene with those of the other members of the MC29 subgroup, and we present evidence from n[r] ...
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Class I defective herpes simplex virus DNA as a molecular cloning vehicle in eucaryotic cells.
... DNA from cells infected with virus from cell transfected with HindilI-cleaved pDHD-6 and helper DNA (the same DNA preparation analyzed in Fig. 5, lanes 1 through 4) was cleaved with EcoR[r] ...
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Avian oncovirus MH2: molecular cloning of proviral DNA and structural analysis of viral RNA and protein.
... Avian acute leukemia viruses MC29 and MH2 share specific RNA sequences: evidence for a second class of transforming genes. Restriction[r] ...
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Molecular cloning of the Harvey sarcoma virus circular DNA intermediates. II. Further structural analyses.
... In the present study, X-HaSV I (6.6-kbp DNA insert), which contains three 650-bp copies of terminal sequences, was doubly plaque purified in recA-. cells and then grown in recA- E[r] ...
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Molecular cloning of human synovial cell collagenase and selection of a single gene from genomic DNA
... J Clin Invest. 1987;79(2):542-546. https://doi.org/10.1172/JCI112845. We used a subclone of a rabbit genomic clone for collagenase that cross-hybridizes with human synovial cell messenger RNA (mRNA) to identify a human ...
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