DNA concentration
ISOLATION AND DETERMINATION OF DNA CONCENTRATION FROM HAIR
... same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA ...
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Observational study on variability between biobanks in the estimation of DNA concentration
... participants. DNA was extracted, diluted and despatched as ...containing DNA solutions at nomi- nal concentrations known only to the coordinating centre were despatched to 15 ...estimate DNA ...
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The influence of DNA concentration in the experiment of DNA damage induced by 7 Li ions and γ rays
... different DNA concentrations in the absence of mannitol except the concentration of 10 ng/ul since the fraction of linear form is 100% at this ...per DNA averagely increases as the decreasing ...
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Circulating free DNA concentration as a marker of disease recurrence and metastatic potential in lung cancer
... cf DNA concentrations as measured by QIAGEN kit was significantly higher in patients who died in the follow‑up period compared with alive patients (P = ...cf DNA concentrations and tumor stage (P = ...cf ...
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Pullulan: a new cytoadhesive for cell-mediated cartilage repair
... (B) An increase in MSC DNA concentration as assayed by PicoGreen incorporation was observed in all culture conditions between days 1 and 3, with a significant decrease in DNA concentrati[r] ...
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Fahrmeir, Julia (2007): Development of purification methods for tumor targeted polyplexes applied in vivo. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... with DNA concentrations between 100 – 400 µg/ml were loaded into the sample ...to DNA binding of labeled and non-labeled ...and DNA respectively PEI and PEI- conjugate concentration was ...a ...
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Transfection of Escherichia coli Spheroplasts IV. Transfection of rec+ and rec− Spheroplasts by Native, Denatured, and Renatured T5 Bacteriophage DNA After Repair of Single-Strand Breaks by Polynucleotide Ligase
... One possible explanation for the two-hit nature of the plot of infective centers versus DNA concentration is the extreme shear sensitivity of T5 phage DNA 1, 13; if the DNA were broken i[r] ...
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Concentration and Purity DNA Spectrophotometer: Sodium Monofluorophosphate forensic impended effect
... forensic DNA profiling, DNA concentra- tion and purity parameters assurance remain important in order to have desirable ...logged DNA bio- logical samples with SMSP revealed associated ...through ...
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Defective Bacteriophage PBSH in Bacillus subtilis
... The kinetic order of renaturation of denatured DNA is not affected by the DNA concentration in the reaction mixture in the range of 1.7 to 77 jig/ml or by 150I iC cal separation of phage[r] ...
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Biases underlying species detection using fluorescent amplified-fragment length polymorphisms yielded from roots
... in DNA extrac- tion, amplification, and quantification of amplified frag- ...root DNA, and this relationship has been investigated towards develop- ing quantitative real-time PCR methods and detection ...
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Genotoxic and epigenotoxic effects in mice exposed to concentrated ambient fine particulate matter (PM2.5) from São Paulo city, Brazil
... tated DNA was collected into tubes containing 4 mL of 10 mM Tris buffer, 1 mM deferoxamine, pH ...The DNA was again precipitated by adding 8 mL of absolute ethanol and ...The DNA concentration ...
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DETERMINATION OF THE MOL% G+C VALUES OF BACTERIAL DNA ISOLATED FROM AL BAIJY OIL REFINERY WORKERS, IRAQ
... and concentration because when working with DNA, it is often important to know its concentration, and to prepare a concentration ranges between 5 – 20 µg/ml for each tested sample suitable for ...
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Standardization of DNA Extraction Method from Mature Dried Leaves and ISSR PCR Conditions for Melia dubia Cav —A Fast Growing Multipurpose Tree Species
... good DNA (Figure 1, Lane ...quality DNA (Figure 1, Lane 7). Further increasing the concentration of NaCl to ...of DNA much (Figure 1, Lane 8). The DNA quantity and quality was further ...
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Propagation of elastic waves in DNA
... It has been found that DNA duplex in high salt concentration can exist either in a left handed conformation or in an overwound right handed C form of DNA depend-.. It is well known that [r] ...
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Absence of Spiroplasma or Other Bacterial 16S rRNA Genes in Brain Tissue of Hamsters with Scrapie
... in DNA from the well-characterized ham- ster 263K scrapie model, which produces predictably high con- centrations of TSE infectivity in the brains of clinically affected ...bacterial DNA at about 1% of the ...
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DNA binding and photocleavage studies of cobalt (III) ethylenediamine complexes: [Co(en)2phen]3+ and [Co(en)2bpy]3+
... between DNA and ...buffered DNA solution (concentration of ∼ 5 to 10 mM in base pairs) were added to each cuvette (reference and sample) to eliminate the absorbance of DNA ...complex ...
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Efficient Genotyping of KRAS Mutant Non-Small Cell Lung Cancer Using a Multiplexed Droplet Digital PCR Approach.
... genomic DNA extracted from KRAS mutant cell lines and tested on DNA extracted from fixed tumour tissue from a cohort of lung cancer patients without prior knowledge of the specific KRAS ...
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Evaluating Population Origins and Interpretations of Identity: a Case Study of the Lemba of South Africa
... The ribozymes were transcribed as previously discussed. The transcribed ribozymes were purified by centrifugation using a membrane (3000 Dalton cutoff). The concentrated samples were adjusted to the same molar ...
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Correlation of cell free DNA plasma concentration with severity of non alcoholic fatty liver disease
... total DNA extracted from 200 µL whole blood with QIAmp Blood DNA Mini kit as described ...and concentration was meas- ured using the Nanodrop 2000 (Thermo Fisher Scien- tific ...then ...
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Threshold Methotrexate Concentration for In Vivo Inhibition of DNA Synthesis in Normal and Tumorous Target Tissues
... methotrexate concentration was 10 -8 M or less, irrespective of the dose administered, while 50% recovery of DNA synthesis in intestinal epithelium was not observed until plasma methotrexate levels were 5 × ...
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