DNA fragment
Differentiation of Campylobacter jejuni and Campylobacter coli strains by using restriction endonuclease DNA profiles and DNA fragment polymorphisms
... chromosomal DNA fragment patterns from a total of 169 Campylobacter jejuni and Campylobacter coli isolates from poultry and humans were analyzed by using DNA restriction endonucleases ClaI and ...The ...
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DNA anti DNA immune complexes Antibody protection of a discrete DNA fragment from DNase digestion in vitro
... to DNA. We therefore compared the ability of F(ab')2 and Fab' to protect DNA from DNase digestion and demonstrated that the bivalent F(ab')2 fragments were protective, but that the univalent Fab' fragments ...
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One-step DNA Fragment Assembly and Circularization for Gene Cloning
... “TA cloning” is a popular method of cloning without the use of restriction enzymes; instead, PCR products are amplified with only Taq DNA polymerase and other polymerases. These polymerases lack 5'-3' proofreading ...
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Candida albicans and Candida stellatoidea specific DNA fragment
... The candidal fragment was radiolabeled and used to probe Southern blots of DNA from several Candida species, various other fungi, and mouse and human cells.. albicans and a strain of Can[r] ...
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A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error free assembly of long DNA fragments
... long DNA fragment with an accurate ...for DNA fragments exceeding 3 ...target fragment is divided into 500–1000-bp modules, or consecutive sub- fragments, each starting and ending with ...
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Characterization of Specific RAPD Markers of Virulence in Tri-chomonas vaginalis Isolates
... In population biology, both direct and non- direct techniques are used for the identifica- tion of the genetic source of variation and the genetic locus of disease or quality strain. The non-directed approaches used ...
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Comparison of nuclease digestion of polyoma virus nucleoprotein complex and mouse chromatin.
... Lengths of DNA fragments obtained from digestion ofpolyoma and mouse chromatin with micrococcal nuclease" DNA fragment Length of DNA fragment base pairs Polyoma virions Mouse nuclei 0.3b[r] ...
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Transcription map for adenovirus type 12 DNA.
... To map the viral DNA sequences complementary to late RNA, 3H-labeled 1- and r-strand viral DNA sequences selected by hybridization to late cytoplasmic RNA were hybridized to DNA fragment[r] ...
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De novo assembly of potential linear artificial chromosome constructs capped with expansive telomeric repeats
... centromeric DNA with telomeric DNA ...telomeric DNA used in AC constructs may contain sequence motives required for DNA ...centromeric DNA fragment as the backbone of the ...
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Gene Replacement With Linear DNA Fragments in Wild-Type Escherichia coli: Enhancement by Chi Sites
... One h e a r DNA fragment is sufficient to produce one gene replacement: The model presented in the Introduction and Figure 1 supposes that a single linear DNA fra[r] ...
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Diagnosis of Chlamydia trachomatis cervical infection by detection of amplified DNA with an enzyme immunoassay
... The outer set of primers was used in amplification reactions with clinical samples or stock strains, and PCR yielded a 280-bp DNA fragment when chlamydial DNA was present in the sample..[r] ...
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Metal-Ligand Exchange Kinetics in Platinum and Ruthenium Complexes2-11
... In early mechanistic studies considerable atten- tion was given to the possibility of rapid S-donor ligand binding to the platinum species, perhaps as an intermediate (41–43) in transport to the DNA in the ...
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Protein Synthesis Worksheet.docx
... Sometimes when DNA is copied errors occur. We call these mutations. Sometimes mutations cause only minor changes to a gene and therefore make only minor changes in the protein produced from that gene. These types ...
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Herpes simplex virus 1 recombinants with noninverting genomes frozen in different isomeric arrangements are capable of independent replication.
... ii Hybridization of electrophoretically separated digests of RBMu3 DNA with 32P-labeled DNA of a plasmid containing a single a sequence confirmed the absence of the BamHI SP fragment spa[r] ...
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Cytogenetic and molecular localization of tipE: a gene affecting sodium channels in Drosophila melanogaster.
... Through chromosome walking and transformation rescue experiments with genomic DNA fragments ranging in size from 42 to 7.4 kb, we identified a fragment of DNA containing [r] ...
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Molecular analysis of variant plasmid forms of a bivalent Salmonella typhi Shigella sonnei vaccine strain
... Since restriction fragment analysis had indicated that DNA rearrangements might be occurring in the vaccine plasmid, large plasmid DNA isolated from the clonal variants was digested with[r] ...
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-RFLP, -RAPD -AFLP,
... RAPD uses short oligonucleotide primers with arbitrary sequences for the PCR amplification. Randomly amplified polymorphic DNA, or RAPD, marker analysis utilizes short PCR primers consisting of random sequences ...
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Biochemical Studies on Bovine Adenovirus Type 3 IV. Transformation by Viral DNA and DNA Fragments
... A31 cell lines transformed with intact BAV3 DNA, EcoRI D fragment DNA, or ligated DNA of HindIII J and E fragments had some characteristics common to BAV3-transformed lines, i.e., decrea[r] ...
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Destruction of Escherichia coli and Broad Host Range Plasmid DNA in Treated Wastewater by Dissolved Ozone Disinfection under Laboratory and Field Conditions
... dissolved ozone delivery system (BlueInGreen LLC, Fayetteville, AR, USA) designed to treat approximately 3.8 million liters per day (MLD) of wastewater was in- stalled in a temporary sidestream just before the disinfec- ...
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Lack of AKR ecotropic provirus amplification in AKR leukemic thymuses.
... A MCF-13 MuLV linear DNA hybridized with the 400bp fragment; B Moloney MuLV linear DNA hybridized with the 400-bp fragment; C MCF-13 MuLV linear DNA hybridized with the 4.8-kbp fragment;[r] ...
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