DNA glycosylase
Evidence that the UV endonuclease activity induced by bacteriophage T4 contains both pyrimidine dimer-DNA glycosylase and apyrimidinic/apurinic endonuclease activities in the enzyme molecule.
... 204-210 0022-538X/81/100204-07$02.00/0 Evidence that the UV Endonuclease Activity Induced by Bacteriophage T4 Contains Both Pyrimidine Dimer-DNA Glycosylase and Apyrimidinic/Apurinic End[r] ...
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Construction of a vaccinia virus deficient in the essential DNA repair enzyme uracil DNA glycosylase by a complementing cell line.
... Construction of permanent cell lines carrying the D4R ORF. Due to the host protein shutoff that occurs immediately after infection, early genes, such as D4R, seem to be good candi- dates for complementing studies because ...
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An active alternative splicing isoform of human mitochondrial 8-oxoguanine DNA glycosylase (OGG1)
... Eight alternatively spliced isoforms of human 8-oxoguanine DNA glycosylase ( OGG1 ) are registered with the NCBI. OGG1-1a is present in the nucleus, whereas the other seven isoforms are present in ...
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Islet expression of the DNA repair enzyme 8-oxoguanosine DNA glycosylase (Ogg1) in human type 2 diabetes
... oxidative DNA damage plays a role in the β -cell dysfunction and loss that occurs in human type 2 ...in DNA re- pair (Tyrberg & Levine, unpublished ...the DNA glycosylase 8-OxoG DNA ...
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Human cytomegalovirus uracil DNA glycosylase is required for the normal temporal regulation of both DNA synthesis and viral replication.
... uracil DNA glycosylase and is highly conserved in all ...This DNA repair enzyme excises uracil residues in DNA that result from the misincorporation of dUTP or spontaneous deamination of ...
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Human immunodeficiency virus type 1 Vpr protein binds to the uracil DNA glycosylase DNA repair enzyme.
... We next tested whether addition of UGI, a specific uracil DNA glycosylase inhibitor which forms a stable complex with UNG (6, 51), could affect the UNG-Vpr interaction. Purified UNG D 84 was incubated with ...
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Effects of vaccinia virus uracil DNA glycosylase catalytic site and deoxyuridine triphosphatase deletion mutations individually and together on replication in active and quiescent cells and pathogenesis in mice
... encode DNA polymerase and other enzymes and factors necessary to replicate their double-stranded DNA genomes ...for DNA replication ...its DNA glycosylase activity, though the latter is ...
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Direct measurement of the substrate preference of uracil DNA glycosylase
... specific DNA-modifying enzymes, and sequence-inde- pendent DNA-modifying ...cognate DNA sequence, a sequence-specific enzyme can simply wait for the thermal breathing motion of the DNA to flip ...
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The role of thymine DNA glycosylase (TDG) and DNA demethylation in TGF beta signaling
... successive DNA replications, as is the case for 5hmC during preimplantation development (Inoue and Zhang, 2011; Wu and Zhang, ...thymine DNA glycosylase (TDG) (He et ...
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Demonstration of pyrimidine dimer-DNA glycosylase activity in vivo: bacteriophage T4-infected Escherichia coli as a model system.
... Our results show that PD-DNA glycosylase activity in vivo can be detected after infection with phage T4 denV+ and that this activity accounts quantitatively for all thymine-containing PD[r] ...
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Quantitative assessment of the effect of uracil DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription PCR
... Uracil-DNA glycosylase (UNG) is a DNA repair enzyme that will cleave uracil-containing DNA while leaving the natural, thymine-containing DNA unaffected ...Amplicon DNA that has ...
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Architecturally diverse proteins converge on an analogous mechanism to inactivate Uracil DNA glycosylase
... Uracil-DNA glycosylase (UDG) compromises the replication strategies of diverse viruses from unre- lated ...UDG DNA-binding cleft, whereas the dimer interface forms a hydrophobic box to trap a ...
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Requirement for Uracil-DNA Glycosylase during the Transition to Late-Phase Cytomegalovirus DNA Replication
... into DNA arises through misincorpo- ration of dUTP by DNA polymerase (2, 51, 54) or from spon- taneous deamination of cytosine creating U:G base pair mis- matches that resolve into A:T transition mutations ...
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Aag DNA Glycosylase Promotes Alkylation-Induced Tissue Damage Mediated by Parp1
... repairing DNA alkylation damage, under certain conditions, initiation of BER can be ...alkyladenine DNA glycosylase (AAG) mediates alkylation-induced tissue damage and whole-animal lethality ...
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A nucleobase lesion remodels the interaction of its normal neighbor in a DNA glycosylase complex
... in DNA pose a persistent threat to the genomic integrity of all living organisms ...replicative DNA polymerization (4), ultimately giving rise to a G:C to T:A transversion ...base-excision DNA repair ...
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Uracil DNA glycosylase specifically interacts with Vpr of both human immunodeficiency virus type 1 and simian immunodeficiency virus of sooty mangabeys, but binding does not correlate with cell cycle arrest.
... uracil DNA glycosylase in human cells (24). Uracil DNA glycosylase cleaves DNA that contains uracil and is involved in base excision ...of DNA damage repair, it was pos- sible ...
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Uracil DNA Glycosylase Is Dispensable for Human Immunodeficiency Virus Type 1 Replication and Does Not Contribute to the Antiviral Effects of the Cytidine Deaminase Apobec3G
... single-stranded DNA, compared to a control EBV-transformed B-cell line that is WT for the UNG gene ...uracil DNA glycosylase encoded by the ...
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Characterization of the Uracil-DNA Glycosylase Activity of Epstein-Barr Virus BKRF3 and Its Role in Lytic Viral DNA Replication
... Several lines of evidence from the present study indicate that EBV BKRF3 encodes a structurally and functionally conserved uracil-DNA glycosylase of the UNG family. First, amino acid sequence alignment ...
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Conserved Domains Required for DEMETER 5-Methylcytosine DNA Glycosylase/lyase Function in planta.
... HhH glycosylase domain and the FLC iron sulfur cluster loop, spanning across the A and the Glycosylase domains, followed by the RRM motif (Figure ...domains), DNA binding (AT- hook motif), and the ...
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den V gene of bacteriophage T4 determines a DNA glycosylase specific for pyrimidine dimers in DNA.
... In support of this two-step mechanism, we found that our preparations of T4 endonuclease V contained a DNA glycosylase activity that produced alkali-labile sites in irradiated DNA and an[r] ...
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