DNA-mediated gene transfer
Differential diagnosis of mut and cbl methylmalonic aciduria by DNA mediated gene transfer in primary fibroblasts
... using DNA-mediated gene transfer of a methylmalonyl CoA mutase cDNA ...clone. Gene transfer of a functional methylmalonyl CoA mutase cDNA clone into mut fibroblasts reconstitutes ...
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DNA-mediated gene transfer in Friend leukemia cells by cotransfection of simian virus 40 DNA with herpes simplex virus thymidine kinase DNA.
... Expression of SV40 T-antigen in FLCs after virus infection, and expression of SV40 T-antigen and HSV-TK gene after cotransfection with calcium phosphate-DNA precipitates in Friend leukem[r] ...
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Kinetics of Recombinant Adeno-Associated Virus-Mediated Gene Transfer
... rAAV) DNA was isolated from infected cells at various times postinfection ...genomic DNA fraction was obtained by proteinase digestion of cells as described previously (45, ...Stringy DNA (HMW ...
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AGROBACTERIUM-MEDIATED GENE TRANSFER RESULTS MAINLY IN TRANSGENIC PLANTS TRANSMITTING T-DNA AS A SINGLE MENDELIAN FACTOR
... T h e segregation of two independent kanamycin resistance loci in the prog- eny of five plants was interpreted as the consequence of independent transfor- mations of [r] ...
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Canine Adenovirus Vectors: an Alternative for Adenovirus-Mediated Gene Transfer
... viruses. DNA preparations, restriction enzyme digests, and Southern blot analysis were performed under standard conditions ...and transfer plasmids, pCAVGFP and ptGFP, are available on ...
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A study of neuronal precursors using retrovirus-mediated gene transfer
... the transfer of newly synthesised viral DNA strands during the reverse transcription process (see ...viral DNA strand, where, together with the R sequences, they form what are called Long Term ...
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Ultrasound-mediated gene transfer (sonoporation) in fibrin-based matrices: potential for use in tissue regeneration
... for gene transfer, it appears to harbour all the requisite attributes outlined above as an ideal gene transfer system for use in GAMs, since it is non-invasive, does not involve the use of ...
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DNA-Mediated Charge Transfer Between [4Fe-4S] Cluster Glycosylases
... Variations in experiments. First, the number of lac + revertants produced by a given sample of CC104 seems to rely upon the genotype of that strain. Even subtle, non- detectable, genetic differences can lead to ...
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Hepatocyte-targeting gene transfer mediated by galactosylated poly(ethylene glycol)- graft-polyethylenimine derivative
... Abstract: Biscarbamate cross-linked polyethylenimine derivative (PEI-Et) has been reported as a novel nonviral vector for efficient and safe gene transfer in our previous work. However, it had no ...
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Engineered DNA-Mediated Antibody Gene Transfer for Prophylaxis Against Infectious Diseases
... isolates. These studies were later extended to delivering anti-simian immunodeficiency virus (SIV) antibody-like immunoadhesins to rhesus macaques using self- complementary AAV1 (scAAV1) vectors, resulting in ...
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Efficient gene transfer into nondividing cells by adeno-associated virus-based vectors.
... vCWR:4Gal was used to compare AAV-mediated gene transfer in vitro into actively replicating cells, cells arrested in mitosis by treatment with the DNA synthesis inhibitors fluorodeoxyuri[r] ...
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Gene transfer technologies leading to transgenic animals
... introduced gene (s) in its germ line is called transgenic ...of gene (s) into animl cells that leads to the transmission of the input transgene to successive generation is called ...the gene ...
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<p>The Potential Role of Epigenetic Drugs in the Treatment of Anxiety Disorders</p>
... of DNA capable of moving to new ...in DNA methylation and histone mod- i fi cations relatively few studies to date have investigated the role of ncRNAs in the pathogenesis of ADs (Tables 1 and ...
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Adenovirus mediated gene transfer into normal rabbit arteries results in prolonged vascular cell activation, inflammation, and neointimal hyperplasia
... arterial gene transfer, and are currently in use as therapeutic agents in animal models of vascular ...of gene transfer into a normal artery, we examined potential vascular cell activation, ...
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Reprogramming The Retina: Next Generation Strategies Of Retinal Neuroprotection And Gene Therapy Vector Potency Assessment
... NRF2 gene transfer in experimental optic ...apoptotic gene expression and thus improving cell viability (Luo et ...that gene augmentation of NRF2 or SIRT1 within RGCs could ameliorate ...
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Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate
... reporter gene to delineate the optimal period for subsequent HSV-tk gene transfer in synchronized cells, we focused our investigation for the transfer of the suicide gene HSV-tk in a ...
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Low efficiency of percutaneous adenovirus mediated arterial gene transfer in the atherosclerotic rabbit
... Accordingly, this model was used in the present study to: a investigate the feasibility of percutaneous, adenovirus-mediated gene transfer to atherosclerotic lesions; and b compare the t[r] ...
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Increased gene expression after liposome mediated arterial gene transfer associated with intimal smooth muscle cell proliferation In vitro and in vivo findings in a rabbit model of vascular injury
... Arterial gene transfer represents a novel strategy that is potentially applicable to a variety of cardiovascular ...arterial gene transfer using nonviral vectors have been compromised by a low ...
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Protection from reoxygenation injury by inhibition of rac1
... Gene transfer efficiencies were assessed after infection with Ad. b GAL, an adenovirus, which encodes the Escherichia coli LacZ gene product (23). Histochemical X-gal staining of Ad. b GAL-infected ...
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Restoration of Spermatogenesis by Adenoviral Gene Transfer into Injured Spinal Cords of Rats
... ago, gene of the environment through which axons must ...Today, gene therapy is being considered for both neurological and reproductive disorders that are not due to genetic ...abnormalities. Gene ...
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