DNA Polymerase
Biochemical and functional characterization of Plasmodium falciparum DNA polymerase δ
... malarial DNA polymerase, which is an enzyme directly involved in polymerization of deoxynucleotides dur- ing replication and/or repair of cellular genetic material ...namely, DNA polymerase α ...
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Formal models of the extension activity of DNA polymerase enzymes
... short DNA strand, called primer, that is Watson-Crick complemen- tary to the template and binds to ...a DNA poly- merase then extends the primer, at one of its ends only, by adding individual nucleotides ...
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Human cytomegalovirus. III. Virus-induced DNA polymerase.
... Activated calf thymus DNA was used as the template primer for the host cell enzyme a and b, and polydA .oligodT,2 18 was used in virus-induced DNA polymerase assays c and d.. infection w[r] ...
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DNA Polymerase in Virions of a Reptilian Type C Virus
... Imnmunological experiments also to assay the partially purified viral DNA polymerase suggested that the viper virus possesses a group- also contained 2 ug of the synthetic template poly [r] ...
5
Discovery of cyanophage genomes which contain mitochondrial DNA polymerase
... ‘DNA polymerase gamma’ was performed on the NCBI database and sequences were selected to include in the ...mtDNA polymerase sequences and not those of other eukaryotes such as plants or ...(e.g. ...
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Translational regulation of herpes simplex virus DNA polymerase.
... Analysis of the transcript of the herpes simplex virus DNA polymerase gene provides evidence that polymerase expression is inefficient at the level of translation.. The kinetics of expre[r] ...
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Characterization of the DNA polymerase gene of human herpesvirus 6.
... We have used the same strategy to generate probes to map and isolate the gene for the DNA polymerase gene of HHV-6: the DNA sequences of the pol genes from five human herpesviruses are k[r] ...
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DNA Polymerase Associated with Human Hepatitis B Antigen
... Figure 5C shows a parallel experiment in which a DNA polymerase reaction with concentrated HBAg and 3H-TTP as described in Table 1 was carried out for 3 h in the presence of NP40 and mer[r] ...
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Structure of Hepatitis B Dane Particle DNA and Nature of the Endogenous DNA Polymerase Reaction
... Because the endogenous DNA polymerase activity in Dane particles was said to introduce radioactive nucleotides into the same restriction endonuclease Hae III-generated DNA fragments as d[r] ...
9
Xenobiotic Nucleic Acid (XNA) synthesis by Phi29 DNA Polymerase
... BASIC PROTOCOL 1 1,5-Anhydrohexitol Nucleic Acid HNA SYNTHESIS BY Phi29 DNA polymerase Template-dependent HNA synthesis consists of the extension of a fluorescently labeled primer pre-an[r] ...
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Molecular genetic analysis of vaccinia virus DNA polymerase mutants.
... DISCUSSION To gain insight into the structure and function of the vaccinia virus DNA polymerase, we have conducted a molecular genetic analysis of one drug-resistant and two temperature-[r] ...
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Biochemical Analysis of Human DNA Polymerase η Structure and Fidelity
... human DNA polymerase η (pol η) results in the hereditary condition known as xeroderma pigmentosum variant (XPV) (Johnson et ...of polymerase function that lacks the nuclear location signal necessary ...
157
Taq2000 DNA Polymerase, Part Number Taq2000 DNA Polymerase, Part Number X Taq Polymerase Buffer
... Protective measures Taq2000 DNA Polymerase Put on appropriate personal protective equipment (see Section 8). Do not ingest. Avoid contact with eyes, skin and clothing. Avoid breathing vapor or mist. Keep in ...
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Pheasant virus DNA polymerase is related to avian leukosis virus DNA polymerase at the active site.
... The determinants shared by ALV and SNV DNA polymerases are different from those shared by APV DNA polymerase and ALV DNA polymerase, since SNV DNA polymerase cannot compete in a heterolo[r] ...
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Relationship of Bacillus subtilis DNA polymerase III to bacteriophage PBS2-induced DNA polymerase and to the replication of uracil-containing DNA.
... HITZEMAN AND PRICE using uracil- versus thymine-containing DNAs and deoxyribonucleoside triphosphates DNA polymerase asa conditions' DNA" dNTP' Reduced HPUrad Relative activity' Percent [r] ...
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DNA Polymerase Fidelity: From Genetics Toward a Biochemical Understanding
... of DNA polymerase that, in turn, stimulated the development of model systems for the study of DNA polymerase fidelity in ...of DNA polymerase fidelity in ...of DNA ...
8
Molecular Architecture of Adenovirus DNA Polymerase and Location of the Protein Primer
... (Ad) DNA polymerase (pol) belongs to the distinct subclass of the pol ␣ family of DNA pols that employs the precursor terminal protein (pTP) as ...reduced polymerase and exonuclease ...
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Dissecting the Fidelity of Bacteriophage RB69 DNA Polymerase: Site-Specific Modulation of Fidelity by Polymerase Accessory Proteins
... B-family DNA polymerase (RB69 gp43) with an associated proofreading 3⬘ ...the polymerase active site (Pol M Exo ⫹ ), and the double mutator Pol M Exo ⫺ ...
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Identification of Conserved Residues Contributing to the Activities of Adenovirus DNA Polymerase
... RB69 DNA Pol has been deter- mined in the absence of substrates and displays a very simi- lar arrangement of conserved residues at the active site ...RB69 DNA Pol, 29 DNA Pol, human DNA Pol ...
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Frequent exchange of the DNA polymerase during bacterial chromosome replication
... during DNA replication using two independent fluorescence-based methods in living ...that DNA replication is a discontinuous process on both ...to DNA for tens of minutes, preventing disassembly of ...
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