DNA purification for cloning
Cloning, expression, purification and characterization of two diagnostically important proteins of Brucella abortus
... EXPERIMENTAL SECTION B. abortus S99 strain, RBPT and STAT reagents were obtained from Indian Veterinary Research Institute, Izatnagar, India. Escherichia coli M15 and BL 21(DE3) for recombinant protein expression were ...
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Molecular Cloning, Expression And Purification Studies With An ORF Of Mycobacterium Tuberculosis
... control DNA isolated from blue ...for purification of expressed ...incubation. Purification analysis showed that purified protein was obtained when we used imidazole concentration at 250 ...
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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
... Pwo DNA Polymerase is a thermostable polymerase with an extremely low error rate (18-fold lower than Taq DNA Polymerase) (7, ...successful DNA extraction and recombinant production of thermostable ...
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Purification and cloning of the salivary peroxidase/catechol oxidase of the mosquito Anopheles albimanus
... Foster City, CA, USA) and analyzed using an automated ABI sequencer (ABI prism, 377 DNA sequencer; Perkin Elmer). After confirming that the sequence of the PCR product contained the predicted partial sequence of ...
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Purification, Cloning, and Expression of an Apyrase from the Bed Bug Cimex lectularius
... Expression of Cimex Apyrase in COS-7 Cells—COS-7 cells (ATCC CRL 1651) were cultivated in a humidified 37 °C incubator with 5% CO 2 in Dulbecco’s modified Eagle’s medium supplemented with 4 m M glu- tamine, 100 units/ml ...
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Cloning, expression, purification and characterization of a DsbA-like protein from Wolbachia pipientis
... METHODS Cloning Wolbachia pipientis -DsbA1 Wolbachia pipientis wMel strain was maintained in Drosophila melanogaster yw67c23 flies reared on standard corn flour-sugar-yeast medium at ...Wolbachia DNA was ...
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Discovery, Cloning, Expression, Purification, and Characterization of Phosphoglycolate Phosphatase from Staphylococcus aureus
... chromosomal DNA was precipitated with solution III (solutions are the same as above in the midiprep ...plasmid DNA was precipitated with 70% ethanol by freezing and ...
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Use of monoclonal antibodies to facilitate identification, cloning, and purification of Chlamydia trachomatis hsp10
... Because of its strict specificity for chsp10, monoclonal antibody M1.2 was selected for routine use. M1.2 reacted by immunoblot with the hsp10s of several C. trachomatis strains but not with Chlamydia psittaci hsp10 or ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... ⁄T cloning vector after purification using QIA quick PCR purification kit (MBI Fermentas, ...Plasmid DNA was isolated and analyzed by electrophoresis on agarose ...
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Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii
... In the course of the acute disease, some of the SAG family members are clearly immunodominant and induce a strong immune response against the par- asite, during early stages of infection. The surface antigens are ...
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Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
... Plasmid DNA has become an essential element of molecular biology and protein ...plasmid DNA, but typically the purification process starts with small ...in cloning techniques have greatly ...
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Purification of Plasmid DNA
... plasmid DNA, along with the original DNAs used to make the recombinant, presents proof of its genetic ...plasmid DNA from culture volumes as low as 1 mL is called a miniprep ...
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Plasmid DNA purification
... For cultivation of bacterial cells harbouring standard high-copy plasmids, we recommend LB (Luria Bertani) medium. The cell culture should be incubated at 37 °C with constant shaking (200–250 rpm) preferably 12–16 h over ...
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Cloning, Expression, and Purification of HIV-2 gp125
... The envelope of the human immunodeficiency virus (HIV) is the main target for neutralizing antibodies. We report the cloning, purification, and characterization of two recombinant forms of the envelope ...
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Novel G DNA binding proteins from the ciliate Tetrahymena thermophila: purification, characterization, cloning and functional analyses
... Sequence analyses showed that TGP3 is homologous to TGPl, and both proteins share an intriguing sequence pattern which may constitute a novel putative G-DNA binding [r] ...
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Cloning, Expression, Purification, and Crystallization of P aeruginosa ICMP
... The insulin-cleaving membrane protease (ICMP) of P. aeruginosa plays a vi- tal role in the pathogenesis of the bacterium and is therefore characterized as an important bacterial virulence factor. In addition, ICMP also ...
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Cloning, overexpression and purification of Escherichia coli tRNAIle and tRNAVal
... Iz agaroznog gela procjenjena duljina PCR-produkta odgovara očekivanoj veličini umnoženog gena za ValRS – 2,86 kb. Umnoženi fragment pročišćen je i razgrađen istim restrikcijskim enzimima kao i vektor pET28b te je ...
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PLANT DNA PURIFICATION KIT
... Plant DNA Purification Kit is designed for efficient recovery of genomic DNA up to 40 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides or having a lower DNA ...
DNA, RNA, and protein purification
... cells are lysed by incubation in a solution containing large amounts of chaotropic ions. This lysis buffer immediately inactivates virtually all enzymes (e.g., DNases, RNases, proteases, and phosphatases) which are ...
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GENE CLONING AND RECOMBINANT DNA TECHNOLOGY
... Genetically modified rice carrying Xa21 gene were tested in the field and compared with wildtype rice plants. Wildtype rice GM Rice[r] ...
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