DNA-stable isotope probing
Assessing the effect of organic residue quality on active decomposing fungi in a tropical Vertisol using 15N DNA stable isotope probing
... Article Title: Assessing the effect of organic residue quality on active decomposing fungi in a tropical Vertisol using 15N-DNA stable isotope probing.. Year of publication: 2011.[r] ...
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Linking Uncultivated Microbial Populations and Benthic Carbon Turnover by Using Quantitative Stable Isotope Probing
... quantitative DNA stable isotope probing (DNA-qSIP) was used for the first time to link uncultivated popu- lations of bacteria and archaea to carbon turnover in lacustrine surface ...
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Proteomic Stable Isotope Probing Reveals Taxonomically Distinct Patterns in Amino Acid Assimilation by Coastal Marine Bacterioplankton
... technique, DNA stable isotope probing (DNA-SIP), applies metagenomic approaches to whole- community DNA that is separated based on differences in density due to isotopic label ...
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Novel Isoprene-Degrading Proteobacteria From Soil and Leaves Identified by Cultivation and Metagenomics Analysis of Stable Isotope Probing Experiments
... environment, DNA stable isotope probing (DNA-SIP) with 13 C-labeled isoprene was used to identify active isoprene degraders associated with soil in the vicinity of a willow ...C-labeled ...
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Targeted metagenomics of active microbial populations with stable-isotope probing
... labeled DNA, in contrast to enrichment culture, may be particularly valuable with environmental samples possessing high diversity yet with unknown culturability of 140 its constituent ...performed DNA-SIP ...
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Stable isotope probing of salicylate-degrading bacteria in polycyclic aromatic hydrocarbon-contaminated soil
... Padmanabhan et al. 2003; DeRito et al. 2005). Stable-isotope probing does have a number of limitations. One major consideration is the availability of 13 C-labeled substrates. Uniform labeling is ...
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Microbial community functioning at hypoxic sediments revealed by targeted metagenomics and RNA stable isotope probing
... Bioinformatics and Biology Insights, 9: 75–88, doi: 10.4137/BBI.S12462 Abstract Advances in next-generation sequencing (NGS) have allowed significant breakthroughs in microbial ecology studies. This has led to the rapid ...
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Stable-isotope probing-based investigations of polycyclic aromatic hydrocarbon-degrading bacteria in contaminated soil
... DNA was extracted from the soil in each flask from sets 1 and 2 in two 500-mg aliquots using the FastDNA ® Spin Kit for Soil (MP Biomedicals, Solon, OH) according to the accompanying instructions with the ...
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Illuminating Microbial Contributions To Soil Carbon Cycling Dynamics Using High Resolution Stable Isotope Probing
... acid stable isotope probing coupled with next generation sequencing, herein high resolution-‐stable isotope probing ...Microcosm DNA was interrogated for 13 C ...
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Characterization of para nitrophenol degrading bacterial communities in river water using functional markers and stable isotope probing
... in the environment. This is partly due to the fact that the previous work has used cultivation- based methods, and these have limitations in their capacity to provide a representative picture of microbial diversity and ...
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Effect of incubation conditions on the enrichment of pyrene-degrading bacteria identified by stable isotope probing in a PAH-contaminated soil
... 3.1.2. Bacterial Community Profiles Parallel FW microcosms containing unlabeled pyrene yielded more concentrated total community DNA extracts than slurry microcosms. A PCR for DGGE was performed using these ...
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Novel bacteria capable of degrading phenanthrene in activated sludge revealed by stable isotope probing coupled with high throughput sequencing
... Discussion DNA-SIP has been successfully used to demonstrate that native microorganisms collected at field sites are engaged in PHE biodegradation (Gutierrez et ...used DNA-SIP and identified six indigenous ...
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Stable Isotope Studies of Fuel Oxygenate Biodegradation.
... of stable isotope probing is that these approaches can detect organisms that are not primarily responsible for metabolism of the isotopically-labeled test substrate but have assimilated labeled ...
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Identifying low pH active and lactate-utilizing taxa within oral microbiome communities from healthy children using stable isotope probing techniques.
... and DNA based Stable Isotope Probing (SIP) to oral plaque communities from healthy children for in vitro temporal monitoring of metabolites and identification of metabolically active and ...
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Critical review on unraveling uncultivable pesticide degraders via stable isotope probing (SIP)
... La imagen apologética favorable a la dinastía reinante enfatiza el fundamento básico de la propaganda pro-Trastámara posterior a la guerra civil: “El reinado de P[r] ...
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Probing the Metabolic Phenotype of Breast Cancer Cells by Multiple Tracer Stable Isotope Resolved Metabolomics
... multiple stable isotope tracers and SIRM to compare the fates of different carbon sources for lipid and central metabolism in estrogen-sensitive breast cancer cells, a triple negative breast cancer cell, ...
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Stable isotope and DNA evidence for ritual sequences in Inca child sacrifice
... n stable light isotope and DNA techniques and to assess the degree of congruence with the Spanish historical accounts that, after Pizarro’s conquest of the Inca capital, Cuzco, in ...
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Genome-Resolved Proteomic Stable Isotope Probing of Soil Microbial Communities Using 13CO2 and 13C-Methanol.
... States Stable isotope probing (SIP) enables tracking the nutrient flows from isotopically labeled substrates to specific microorganisms in microbial ...
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Consuming algal products: trophic interactions of bacteria and a diatom species determined by RNA stable isotope probing
... is stable isotope probing (SIP) which allows an identification of active microorganisms using particular growth ...by isotope arrays coupled with fingerprinting ...
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Probing the metabolic network in bloodstream-form Trypanosoma brucei using untargeted metabolomics with stable isotope labelled glucose
... heavy-atom isotope-labelled glucose has been used to probe the metabolic pathways active in cultured bloodstream form trypomastigotes of Trypano- soma brucei, a parasite responsible for human African ...
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