DNA transfer
Natural Competence Is a Major Mechanism for Horizontal DNA Transfer in the Oral Pathogen Porphyromonas gingivalis
... minimal DNA identity to the W83 traA–Q locus (15, 31); the ATCC 33277 traA–Q gene products are predicted to be more sim- ilar to those found in conjugative Bacteroides ...to transfer plasmid DNA to ...
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Utilization of nonhomologous minus-strand DNA transfer to generate recombinant retroviruses.
... FIG. 1. MLV-based retrovirus vectors used to study minus-strand DNA transfer. (A) Structures of pAR2 and pPY31. Black boxes, Mo-MLV/Mo-MSV LTR sequences including U3, R, and U5; white boxes, SNV LTR ...
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Adenovirus type 12 DNA firmly associates with mammalian chromosomes early after virus infection or after DNA transfer by the addition of DNA to the cell culture medium.
... Ad12 DNA association with chromosomes and interphase nuclei was also observed in BHK21 and T637 hamster cells at 2 ...Ad12 DNA (16, 26, 28) at a single chro- mosomal site (5, 13, ...Ad12 DNA. Upon ...
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THE INFIDELITY OF CONJUGAL DNA TRANSFER IN ESCHERICHIA COLI
... Quite similar spectra for amber mutations are obtained with or without F’ transfer, the exception being an increase in the proportion of mutants isolated at the mutat[r] ...
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Evidence of Extensive DNA Transfer between Bacteroidales Species within the Human Gut
... Selection of genomes for mock-community analysis. 156 genomes identified by NCBI as Bacteroides or Parabacteroides species were retrieved from the RefSeq repository. Genomes from species originating from non- human ...
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Selective assay for herpes simplex viruses expressing thymidine kinase.
... Introduction of herpes simplex virus thymidine kinase gene into mouse cells using virus DNA or transformed cell DNA.. Transfer of thymidine kinase to thymidine kinaseless L cells by infe[r] ...
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Transfer of DNA from Bacteria to Eukaryotes
... host DNA repair ...to transfer DNA to many nonplant cells, from fungi to human cultured cells (37), sug- gesting that T-DNA nuclear import and subsequent processing and integration are ...
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Generation and neutralization of pseudovirions of human papillomavirus type 33.
... We next compared the efficiencies of heavy and light VLPs to mediate the transfer of pSVbgal into COS-7 cells. To use identical amounts of VLPs, the L1 protein of purified VLP preparations was quantitated by ...
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Genetic requirements for the targeted integration of Agrobacterium T-DNA in Saccharomyces cerevisiae
... T-DNA transfer experi- ments with LBA1119(pSDM8001) and a rad52yku70 mutant ...non-homologous DNA can be ligated in vivo to linear fragments of mitochondrial DNA present in the nucleus to form ...
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Efficient initiation and strand transfer of polypurine tract-primed plus-strand DNA prevent strand transfer of internally initiated plus-strand DNA.
... displacement DNA synthesis occur after plus-strand strong-stop DNA ...displacement DNA synthesis (23, 56), although displacement DNA synthesis was not observed in other in vitro studies ...
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Electrochemical studies of electron transfer in DNA films with covalently tethered intercalators
... of DNA in 1953 defines a new era for molecular biology and ...of DNA as the carrier of genetic information in cells, but also implicates that DNA may have some unique physical properties and other ...
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Comparison of DNA lipid complexes and DNA alone for gene transfer to cystic fibrosis airway epithelia in vivo
... gene transfer to murine cells might be different from that required for human ...of DNA from the lipid complex, and entry into the ...to DNA–cationic lipid ...of DNA–LipofectACE complexes by ...
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Immunomodulation by mucosal gene transfer using TGF beta DNA
... b DNA– and control vector– treated animals (on days 0, 7, and 14) were ocularly infected with the RE strain of HSV, 1 d after the last plasmid DNA ex- ...b DNA ad- ministration was effective at ...
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DNA Mediated Charge Transport Signaling Within the Cell
... (kb) DNA strands containing a single base mismatch, which interrupts DNA CT, rather than remaining bound to well-matched DNA ...utilize DNA CT predicts such a redistribution as a first step in ...
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Roles of protons and structure in electron-transfer reactions of DNA and RNA
... and DNA damage (Figure ...both DNA and in RNA by Hoogsten or reverse Hoogsteen base pairing (Figure ...Triplex DNA plays a role in gene regulation by causing DNA replication to pause or stop ...
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Identification of the gene controlling the synthesis of the major bacteriophage T5 membrane protein.
... Mutants in gene Al do not transfer the whole phage DNA molecule, 542 do not break down host DNA after infection, and do not shut off the synthesis of host and pre-early phage proteins.. [r] ...
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Horizontal Gene Transfers in Mycoplasmas (Mollicutes)
... Despite having a minimal genome, several mycoplasmas have retained a form of sexual competence and are able to exchange significant amount of chromosomal material in addition to MGEs. This finding is radically changing ...
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Atom Transfer Radical Polymerization (ATRP)in Amplification-by-Polymerization for DNA Sensing
... The reaction kinetics for traditional ATRP reactions at the absence of DNA has been extensively studied in the past. 12-18 For example, the polarity of the reaction environment, including both surface polarity and ...
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Mosaic pregnancy after transfer of a “euploid” blastocyst screened by DNA microarray
... Although high frequencies of mosaicism have been reported in preimplantation human embryos, especially from women with advanced maternal age [10-12], the frequency of chromosomal mosaicism in spontaneous abortion ...
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Thermodynamics of 5 bromouracil tautomerisation from first principles molecular dynamics simulations
... proton transfer from N3 to O4 – a multi-step process, potentially involving several H atoms – cannot be modelled by a single sequence of trajectories if the same single distance constraint is employed in each of ...
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