DNA vaccination
In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence
... Seven different mammalian expression vectors were com- pared for their ability to drive high levels of HSA protein expression in four different primary horse cells and COS- 7. Two of the vectors, G1 and H1 with the HSA ...
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Elicitation of High-Frequency Cytotoxic T-Lymphocyte Responses against both Dominant and Subdominant Simian-Human Immunodeficiency Virus Epitopes by DNA Vaccination of Rhesus Monkeys
... that vaccination with plasmid DNA, but not vaccination with a live recombinant vector or infection with simian-human immunodeficiency virus (SHIV), elicits potent CTL responses against both dominant ...
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CD4+ T cell–independent DNA vaccination against opportunistic infections
... Kexin DNA vaccination results in protective antibody responses. Due to the fact that we observed protection in the primary challenge with PC, we investigated whether this was antibody mediated. We initially ...
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Co-administration with DNA encoding papillomavirus capsid proteins enhances the antitumor effects generated by therapeutic HPV DNA vaccination
... L2 DNA in combination with DNA vaccines could elicit enhanced antigen-specific CD8 + in both CRT/E7 and ovalbumin (OVA) antigenic ...L2 DNA with CRT/E7 DNA led to the generation of ...
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Therapeutic DNA vaccination of vertically HIV-infected children: Report of the first pediatric randomised trial (PEDVAC)
... HIV-DNA vaccination at week 60 when compared to baseline ...HIV- DNA vaccination can increase the frequency of CD8+ lympho- cytes releasing ...by vaccination or transient viremia. ...
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DNA vaccination against persistent viral infection.
... after DNA immunization is not a finding restricted to this work, and the relative inefficiency of in vivo transfection by the intramus- cular route is leading to the development of novel methods of DNA ...
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Induction of neonatal tolerance by plasmid DNA vaccination of mice
... research. DNA vaccines offer great promise towards this goal, since they are easy to construct and manufacture, and are cost competitive with conventional attenuated and subunit vac- ...a DNA vaccine is ...
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DNA Vaccination by Electroporation and Boosting with Recombinant Proteins Enhances the Efficacy of DNA Vaccines for Schistosomiasis Japonica
... efficacy, vaccination via prime-boost significantly increased vaccine-specific antibody responses over those seen in mice solely vaccinated with plas- mid DNA ...following DNA injection, injected ...
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Influenza Virus H5 DNA Vaccination Is Immunogenic by Intramuscular and Intradermal Routes in Humans
... A DNA vaccine encoding hemagglutinin (HA) protein from the A/Indonesia/5/05 (H5N1) strain was initially tested in two randomized phase I clinical ...i.d. vaccination compared to i.m. vaccination ...
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The efficacy of DNA vaccination is enhanced in mice by targeting the encoded protein to dendritic cells
... the DNA vaccine encoding an HIV gag p41–scFv DEC205 fusion protein, mice were protected from an airway challenge with a recombinant vaccinia virus expressing the HIV gag p41, even with 1% of the dose of ...
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Treatment with Imiquimod enhances antitumor immunity induced by therapeutic HPV DNA vaccination
... CRT/E7 DNA via gene gun on d9, d13, and ...CRT/E7 DNA in combination with imiqui- mod demonstrated significantly lower numbers of myel- oid-derived suppressor cells compared to the other treated ...CRT/E7 ...
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Tumor endothelial marker 1–specific DNA vaccination targets tumor vasculature
... erglasses (BD Biocoat; catalog no. 354085), fixed in formaldehyde, per- meabilized, and stored in 70% ethanol. The single molecule RNA-FISH protocol was carried out as described previously (80). Samples were hybridized ...
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Molecular characterization of the Corynebacterium pseudotuberculosis hsp60 hsp10 operon, and evaluation of the immune response and protective efficacy induced by hsp60 DNA vaccination in mice
... the DNA sequence of paralogs of ...the DNA sequence of the ...genomic DNA, 2 μM of each of the primers, 1 × PCR Buffer II and 1 U AccuPrime taq DNA ...
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DNA vaccination for prostate cancer: key concepts and considerations
... of DNA vac- cines coding multiple TAAs, the authors hypothesise that delivering multiple TAAs will result in a wider and more potent immune response, targeting tumour cells with a syn- ergistic effect ...the ...
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DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice
... Immunoglobulins IgA, IgM and IgE were reported to participate in the immunological responses against T. gondii infection. However, less attention has been placed on these immunoglobulins during vaccination trials ...
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Enhanced T-Cell Immunogenicity and Protective Efficacy of a Human Immunodeficiency Virus Type 1 Vaccine Regimen Consisting of Consecutive Priming with DNA and Boosting with Recombinant Fowlpox Virus
... with DNA and rFPV vectors offers great hope for improved immunoprophylaxis against infections caused by a wide range of pathogens, including ...a vaccination strategy based on consecutive immunization with ...
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Strategies and hurdles using DNA vaccines to fish
... stories. DNA vaccination strategies against many other viral diseases have, however, not yet yielded sufficient results in terms of ...why DNA vaccine strategies against other viral diseases fail to ...
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The application of exosomes as a nanoscale cancer vaccine
... Figure 1 Biological delivery systems. A) Bacteria can be used for gene delivery, and it is used in cancer gene therapy, DNA vaccination, and treatment of some genetic diseases. B) Bacteriophages are viruses ...
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Novel Approaches To Improve DNA Vaccine Induced Responses Against Difficult Infectious Disease Targets
... of DNA vaccines, the adjuvant used is slightly different from traditional ...of DNA vaccination but also to tailor the response towards the desired ...
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Combined skin and muscle vaccination differentially impact the quality of effector T cell functions : the CUTHIVAC 001 randomized trial
... of DNA vaccination influences the quality of the cellular immune ...The DNA plasmid encoded a MultiHIV B clade fusion protein designed to induce cellular ...responses. DNA given by IM + EP ...
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