DNA Vaccine
Ub Combination Enhanced Cellular Immune Response Elicited by HSP65 DNA Vaccine against Mycobacterium tuberculosis
... 2.4. Plasmid Construction and Preparation The cDNA of HSP65 is cloned from the genome of cul- tured Mycobacterium tuberculosis by PCR method. The primers are as following: forward primer 5’gcgc aagctt gccacc atg gcc aag ...
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A DNA Vaccine versus Attenuated Vaccine to Protect against Salmonella Infection in Chickens
... The preliminary results suggested that both live attenuated and DNA vaccines proved to be effective, providing protection to immunized animals. The vaccines were safe and well tolerated by the chickens, in ...
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DNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice
... ABSTRACT. VIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and ...
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Comparison of Five Expression Vectors for the Ha Gene in Constructing a DNA Vaccine for H6N2 Influenza Virus in Chickens
... HA-expressing DNA vaccine appeared to induce better protective responses than higher ...attenuated vaccine responses [9]. Moreover, the possibility that high dose DNA is introduced into ...
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Use of a novel enhanced DNA vaccine vector for preclinical virus vaccine investigation
... Abstract: DNA vaccines are stable, safe, cost effective to produce and relatively quick and easy to ...date DNA vaccines have shown relatively poor immunogenicity in humans despite promising preclinical ...
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Immunotherapy with an HIV-DNA Vaccine in Children and Adults
... HIV-DNA vaccine (HIVIS) has been used in clinical trials in both children and adults with the aim of improving and broadening the infected individuals’ immune ...HIV-DNA vaccine was generally ...
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Enhancing DNA vaccine potency by coadministration of DNA encoding antiapoptotic proteins
... E7 DNA mixed with BCL-xL exhibited the fewest number of pulmonary tumor nodules ...E7 DNA mixed with caspase-3 (11.6 ± 4.7), E7 DNA mixed with pSG5 (no insert) ...of DNA encoding either XIAP ...
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Antitumor Effects of HPV DNA Vaccine Adjuvanted with Beclin-1 as an Autophagy Inducer in a Mice Model
... During the last years, several attempts have been made to promote the efficiency of immunostimulatory adjuvant system in vaccine development. Given the ability of autophagy to elicit humoral and cellular immunity, ...
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Novel Approaches To Improve DNA Vaccine Induced Responses Against Difficult Infectious Disease Targets
... enabled DNA to induce humoral responses in NHP and ...two vaccine antigens by ELISA after receiving a DNA vaccine for HPV ...with DNA alone, improvements are still needed, especially ...
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Prime-Boost Immunization Using a DNA Vaccine Delivered by Attenuated Salmonella enterica Serovar Typhimurium and a Killed Vaccine Completely Protects Chickens from H5N1 Highly Pathogenic Avian Influenza Virus
... mucosal DNA vaccine delivered by Salmonella and a boost with conventional killed ...of DNA vaccine and traditional protein vaccine and fully activates the whole immune system, including ...
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Designing a DNA Vaccine-based Leishmania major Polytope (Preliminary Report)
... efficient vaccine against all stages of Leishma- nia major lifetime along with having inhibitory impact on the entrance of parasite into target ...this DNA vaccine may pro- tect the cells by ...
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A DNA Vaccine against Escherichia coli O157:H7
... stranded DNA, more controversy is seen in the effect of plasmid ...of DNA were used in intramuscular immunization with no prior treatment or adjuvant addition in order to achieve maximal immune ...vivo ...
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Construction of Mtb72F Plasmid as a DNA Vaccine Candidate for Mycobacterium tuberculosis
... and DNA vaccines, have been produced. A DNA vaccine could be effective in controlling the ...disease. DNA vaccines, which often stimulate cellular immunity and both TCD8+ and TCD4+ responses, ...
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Original Article Polyethylenimine modified nanoparticle adjuvant increases therapeutic efficacy of DNA vaccine Ag85A-ESAT-6-IL-21 in mice infected with Mycobacterium tuberculosis
... Figure 1. Histology evaluation of lung tissue pathological change in M. tuberculosis infected mice treated with the different vaccines. Profile of the pathological change of lung tissues in mice that were infected with ...
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Review Article Mucosal anti-caries DNA vaccine: a new approach to induce protective immunity against streptococcus mutans
... anti-caries DNA vaccine by inducing chemotactic migration of DCs to secondary lymphoid tissues, which lead- ing to significantly increased level of serum PAc-specific IgG ...anti-caries DNA ...
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Simian immunodeficiency virus DNA vaccine trial in macaques.
... of DNA immunization would have supported the ‘‘maturation’’ of a protective re- ...The DNA vaccine was also unlike live attenuated in- fections in failing to raise persisting titers of neutralizing ...
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Assessment of a DNA vaccine encoding Burkholderia pseudomallei bacterioferritin
... constructed DNA vaccine, pBps190 was transfected into the mammalian cell line HEK ...pBps190 DNA vaccine (Figure ...engineered vaccine contained all the necessary information to direct ...
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Immunization with a suicidal DNA vaccine expressing the E glycoprotein protects ducklings against duck Tembusu virus
... suicidal DNA vaccine, which have been shown to induce caspase-dependent apop- tosis in transfected cells ...suicidal DNA vaccine typically corresponds to the serum levels of pro-inflammatory ...
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Recombinant DNA vaccine technology and their applications in cancer immunotherapy
... based vaccine strategy seems lucrative approach to target several diseases and especially the cancer which express antigens exclusively limited to the tumor ...of DNA vaccine to induce long term ...
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Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector
... caveolae pathway. Although the amount of Tat-phage penetrate into the cells was much higher than the phage without Tat displayed, the gene transfer efficiency medi- ated by Tat peptide was lower than that via ...
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