dominant negative form
Wnt3a mediated chemorepulsion controls movement patterns of cardiac progenitors and requires RhoA function
... active form of RhoA (Ridley and Hall, 1992), did not have a significant effect on the early phases of cardiac progenitor cell migration, whereas at later stages some cells took a wider path ...active form ...
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The IRF-3 Transcription Factor Mediates Sendai Virus-Induced Apoptosis
... a dominant negative mutant of IRF-3 lacking most of the DNA binding domain (IRF-3 ⌬N) was used to examine the involvement of IRF-3 in virus-mediated ...inant negative mutant of IRF-3 has been shown ...
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Differential stability of β catenin along the animal vegetal axis of the sea urchin embryo mediated by dishevelled
... putative dominant negative form of the protein with respect to canonical Wnt signaling (Axelrod et ...because dominant negative approaches have not yet revealed a role for the protein ...
10
Dilated cardiomyopathy in transgenic mice expressing a dominant negative CREB transcription factor in the heart
... a dominant-negative form of the CREB transcription factor (CREBA133) under the control of the cardiac myocyte- specific alpha-MHC promoter develop dilated cardiomyopathy that closely resembles many ...
13
Identification of Domains in Gag Important for Prototypic Foamy Virus Egress
... a dominant negative form of this enzyme results in inhibition of class E VPS-dependent viral ...coexpressed dominant nega- tive forms of Vps4 and the closely related Vps4B/SKD1 (50) as GFP or ...
8
The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline
... a dominant-negative form of mammalian ADAM10 was able to block the Slit-induced relocalization of Sos and its associated change in cell morphology ...
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Down-regulation of p21-activated serine/threonine kinase 1 is involved in loss of mesencephalic dopamine neurons
... DN: dominant-negative form; CA: constitutively active form; Bcl-2: B-cell lymphoma 2; ERK: extracellular signal-regulated kinase; DA: dopamine; SN: substantia nigra; Bcl-xL: B-cell ...
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The Adam family metalloprotease Kuzbanian regulates the cleavage of the roundabout receptor to control axon repulsion at the midline
... a dominant-negative form of mammalian ADAM10 was able to block the Slit-induced relocalization of Sos and its associated change in cell morphology ...
10
Identification and Analysis of Escherichia coli Ribonuclease E Dominant-Negative Mutants
... the dominant-negative variant can form mixed multimers with RNase E in vivo, leading to affinity purification of ...the dominant- negative mutant protein ...smaller ...
9
The MODY1 gene HNF 4α regulates selected genes involved in insulin secretion
... a dominant negative form of HNF-4α was overexpressed in insulinoma cells, several genes involved in glucose metabolism as well as HNF-1α were differentially expressed, suggesting that HNF-4α ...
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C Jun N terminal kinases/c Jun and p38 pathways cooperate in ceramide induced neuronal apoptosis
... a dominant negative form of c-Jun, truncated in its transacti- vation ...of dominant negative c-Jun-expressing neurons with the pharmacological inhibitor of p38 kinase, SB203580, ...
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Molecular effects of novel mutations in Hesx1/HESX1 associated with human pituitary disorders
... The S170L substitution is located immediately C-terminal to the homeodomain in an RESQLF motif, which is completely conserved in, and unique to, HESX1 homeoproteins. Recent structural studies of Pbx1 class homeodomains ...
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Critical roles of c Jun signaling in regulation of NFAT family and RANKL regulated osteoclast differentiation
... transfection. Dominant-negative c-Fos cDNA (31) and an NFATx3 luciferase construct (32) were gifts from Charles Vinson (Nation- al Cancer Institute, Bethesda, Maryland, USA) and David ...a ...
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Dominant Negative Inhibition of Human Immunodeficiency Virus Particle Production by the Nonmyristoylated Form of Gag
... Recent reports suggest that HIV particle assembly and bud- ding take place at the raft-rich area of the plasma membrane (2, 9, 34, 42, 46). Raft association of Gag was examined by similar equilibrium flotation ...
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Use of time lapse imaging and dominant negative receptors to dissect the steroid receptor control of neuronal remodeling in Drosophila
... this dominant negative receptor (Cherbas et ...this dominant negative can bind 20E (Cherbas et ...F645A dominant negative to support derepression, however, may be highly ...
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Endocytic pathways mediating oligomeric Aβ42 neurotoxicity
... expressing dominant-negative proteins, or knock-down of endogenous ...the dominant-negative dynamin mutant K44A (myc-tag), the dominant-negative AP180 mutant AP180-CT (Flag-tag), ...
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Characterization of Dominant-Negative Forms of Anthrax Protective Antigen
... release, the related property of conversion of the heptamer to an SDS-resistant conformation under acidic conditions, and translo- cation of LFn across the CHO-K1 plasma membrane. The F427K mutation was the weakest of ...
6
Targeted inhibition of p38 MAPK promotes hypertrophic cardiomyopathy through upregulation of calcineurin NFAT signaling
... expressing dominant-negative mutants of p38α, MKK3, and ...the dominant-negative transgenic mice after PE stimulation (10 ...three dominant-negative strate- gies significantly ...
13
Dominant-negative inhibitors of EBNA-1 of Epstein-Barr virus.
... the dominant-negative mu- tants of EBNA-1 are likely to inhibit wild-type EBNA-1 when they occupy only a subset of the binding sites on ...its dominant-negative mutants were intro- duced ...
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The dominant-negative interplay between p53, p63 and p73: A family affair
... a dominant-negative effect [20, 25, ...to form tetramers [54] or to its instability in yeast as suggested by its low expression level (Figure ...
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