dominant negative mutants
Identification and Analysis of Escherichia coli Ribonuclease E Dominant-Negative Mutants
... Such mutants could manifest a dominant- negative phenotype by competing with wild-type RNase E to access the same (limiting) ...for dominant-negative mutants did not reveal any ...
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Dominant-negative mutants of human MxA protein: domains in the carboxy-terminal moiety are important for oligomerization and antiviral activity.
... a dominant-interfering effect on the antiviral activity of wild-type MxA rendering MxA-expressing cells susceptible to infection with influenza A virus, Thogoto virus, and vesicular stomatitis ...the ...
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Dominant Negative Mutants of the Murine Cytomegalovirus M53 Gene Block Nuclear Egress and Inhibit Capsid Maturation
... FIG. 4. Flag tagging does not affect the inhibitory features of DN s309. (A) Schematic representation of the tagging strategy. Depicted are the wt M53 ORF domains. The N-terminal epitope recognized by the M53-specific ...
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Identification of dominant-negative mutants of the herpes simplex virus type 1 immediate-early protein ICP0.
... Regulation of the herpes simplex virus type 1 late gamma2 glycoprotein C gene: sequences between base pairs -34 to +29 control transient expression and responsiveness to transactivation [r] ...
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Dominant-negative mutants are clustered in a domain of the human T-cell leukemia virus type I Rex protein: implications for trans dominance.
... To test whether the Rex domain located between amino acids 58 and 65 might be involved in multimerization, we constructed chimeric Rex-Rev proteins by inserting the putative Rev multimer[r] ...
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Pogoda, Madlen (2013): Novel approaches to identify and characterise dominant negative mutants of essential MCMV genes. Dissertation, LMU München: Medizinische Fakultät
... Until recently, the nuclear export pathway exploited by herpesviruses had been thought to be unique to them. However, Speese and colleagues, during their investigation of the Drosophila synaptic development, found ...
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Functional dissection of the human spumaretrovirus transactivator identifies distinct classes of dominant-negative mutants.
... Collectively, these data suggest that the activation defect of the A194-200 protein reflects a lack of sequence information that is critical to the Bell activator function; on the other [r] ...
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Random Screening for Dominant-Negative Mutants of the Cytomegalovirus Nuclear Egress Protein M50
... M50 mutants was made available from this study which relies on the random insertion of 5 aa or a stop codon into the protein coding ...function mutants from this library were now placed under the control of ...
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Identification of Dominant Negative Human Immunodeficiency Virus Type 1 Vif Mutants That Interfere with the Functional Inactivation of APOBEC3G by Virus-Encoded Vif
... identified dominant negative mutants of Vif that interfered with the ability of wild-type Vif to inhibit the encapsidation and antiviral activity of ...These mutants were nonfunctional due to ...
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Ras participates in the activation of p38 MAPK by interleukin 1 by associating with IRAK, IRAK2, TRAF6, and TAK 1
... MAPK. Dominant negative forms of these were found to inhibit activation of p38 MAPK by ...IL-1. Dominant nega- tive RasN17 blocked the effect of the active forms of all but MKK3 and MKK6, indicating ...
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Role of the GTPase Rab1b in Ebolavirus Particle Formation
... whether dominant-negative Rab1b mutants interfere with Ebolavirus virion formation by affecting GBF1, we cloned the N-terminally FLAG-tagged ORF of GBF1 (NM_004193) into ...A ...
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Solution structure of the TLR adaptor MAL/TIRAP reveals an intact BB loop and supports MAL Cys91 glutathionylation for signaling
... as dominant-negative mutants of TLR4 signaling and could not reconstitute MAL-deficient ...minor dominant-negative ef- fects against ...strong dominant-negative effect on ...
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The Survivin saga goes in vivo
... of dominant-negative mutants or by antisense-mediated reductions in SURVIVIN expression has been associated with supernumer- ary centrosomes, aberrant mitosis, and defective cytokinesis, with cells ...
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Human Rhinovirus 14 Enters Rhabdomyosarcoma Cells Expressing ICAM-1 by a Clathrin-, Caveolin-, and Flotillin-Independent Pathway
... markers, dominant negative mutants, and pharmacological inhib- itors allowed us to demonstrate that the major group virus HRV14 enters rhabdomyosarcoma cells transfected to express human ICAM-1 in a ...
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The RNA Helicase eIF4A Is Required for Sapovirus Translation
... and dominant-negative mutants, we found that eIF4A is required for viral translation and infectivity, suggesting that despite the presence of a very short 5= UTR, eIF4A is required to unwind RNA ...
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Identification of Domains in Gag Important for Prototypic Foamy Virus Egress
... of dominant negative Tsg101 and Vps4 proteins on mutant PFV budding revealed discrepancies between the current models of L-domain exploitation of ESCRT machinery and that apparently used by ...a ...
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Rhesus Macaque TRIM5 Alleles Have Divergent Antiretroviral Specificities
... Unsurprisingly, the truncated B30.2 domain of Mamu-7 has no apparent antiretroviral activity when fused to the Mamu-1 RBCC (Fig. 1). Whether Mamu-7 TRIM5 exon 8 is expressed in vivo remains unclear. Similar ...
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COMPETITION INVOLVING DOMINANT MUTANTS IN EXPERIMENTAL POPULATIONS OF DROSOPHILA MELANOGASTER
... Flies heterozygous f o r the dominant mutants rather than the homozygous dominant types were used in the tests on longevity and mating success because the critica[r] ...
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Glucocorticoid receptor beta, a potential endogenous inhibitor of glucocorticoid action in humans
... Dominant negative activity of an endogenous thyroid hormone receptor variant alpha 2 is due to competition for binding sites on target genes.. Molecular mechanisms of dominant negative a[r] ...
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Characterization of Dominant-Negative Forms of Anthrax Protective Antigen
... a dominant-negative (DN) phenotype, converting it into a potent ...The mutants were made by site-directed mutation of the cloned form of PA in Escherichia coli and tested by various assays conducted ...
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