Double-stranded RNA-Dependent kinase (PKR)
The Cellular TAR RNA Binding Protein, TRBP, Promotes HIV-1 Replication Primarily by Inhibiting the Activation of Double-Stranded RNA-Dependent Kinase PKR
... of PKR activity abrogates the ability of TRBP to enhance HIV-1 ...of PKR activation, we first sought to confirm that physiological levels of TRBP are sufficient to affect viral gene ...of PKR ...
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A Map of the Arenavirus Nucleoprotein-Host Protein Interactome Reveals that Junín Virus Selectively Impairs the Antiviral Activity of Double-Stranded RNA-Activated Protein Kinase (PKR)
... negative-strand RNA viruses that cause sig- nificant human ...the double-stranded RNA (dsRNA)-activated protein kinase (PKR), a well-characterized antiviral protein that in- ...
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The VP35 Protein of Ebola Virus Inhibits the Antiviral Effect Mediated by Double-Stranded RNA-Dependent Protein Kinase PKR
... infection, PKR forms a homodimer and autophosphorylates on multiple serine, threonine, and tyrosine residues (17, 18, ...convert PKR into an active enzyme that phos- phorylates eIF-2 ␣ at serine ...(C) ...
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NSs Protein of Rift Valley Fever Virus Induces the Specific Degradation of the Double-Stranded RNA-Dependent Protein Kinase
... against PKR (monoclonal antibody PKR B-10 [Santa Cruz], diluted 1:1,000), RVFV N protein (mouse polyclonal anti-RVFV N, diluted 1:1,000), phosphor- ylated eIF-2␣ (rabbit polyclonal anti-Ser51 [Biosource], ...
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The Dominant-Negative Inhibition of Double-Stranded RNA-Dependent Protein Kinase PKR Increases the Efficacy of Rift Valley Fever Virus MP-12 Vaccine
... of PKR into MP-12. rMP12- mPKRN167 encodes a dominant-negative PKR (the N-terminal 167 amino acids of mouse PKR) in place of NSs, which is analo- gous to human ...PKR⌬E7. PKR⌬E7 is ...
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Sendai Virus C Protein Plays a Role in Restricting PKR Activation by Limiting the Generation of Intracellular Double-Stranded RNA
... of double-stranded RNA (dsRNA) generation for maintaining the rate of protein synthesis in infected ...of PKR, since knockdown of PKR by small interfering RNA limited eIF2 ␣ ...
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Toscana Virus NSs Protein Promotes Degradation of Double-Stranded RNA-Dependent Protein Kinase
... of PKR is not required for PKR degrada- tion by TOSV ...of PKR was required for its TOSV NSs-mediated downregu- ...vitro-synthesized RNA encoding a myc- tagged, kinase-inactivated form ...
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Stress Granules Regulate Double Stranded RNA Dependent Protein Kinase Activation through a Complex Containing G3BP1 and Caprin1
... with PKR. Our data suggest that G3BP1 may interact with PKR and regulate activation at the ...proteins. PKR is known to autophosphorylate itself in bacterial cells, rendering it active; however, ...
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Herpes Simplex Virus Selectively Induces Expression of the CC Chemokine RANTES/CCL5 in Macrophages through a Mechanism Dependent on PKR and ICP0
... of PKR and viral tegument and immediate- early genes in RANTES ...viral RNA is known to be sensed in cells by the double-stranded-RNA-activated protein kinase (PKR) (40), ...
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Human Immunodeficiency Virus Type 1 Tat Protein Activates Transcription Factor NF-κB through the Cellular Interferon-Inducible, Double-Stranded RNA-Dependent Protein Kinase, PKR
... 4. PKR is functionally required for Tat transactivation of the LTR promoter in mouse ...from PKR knockouts were transiently transfected with a reporter plasmid containing the LTR linked to the CAT ...of ...
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The Murine Double-Stranded RNA-Dependent Protein Kinase PKR Is Required for Resistance to Vesicular Stomatitis Virus
... Interferons (IFNs) have for some time now been recognized as the cytokines responsible for conferring an antiviral state on cells of the host. These effects are mediated by the gene prod- ucts activated or induced ...
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Functional Domains and the Antiviral Effect of the Double-Stranded RNA-Dependent Protein Kinase PKR from Paralichthys olivaceus
... evaluating PKR function in mammals (35), we confirm that PoPKR is able to inhibit protein translation in ...WT PKR, the isolated kinase domain ⌬ N PKR also prevents luciferase ...of PKR ...
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West Nile Virus-Induced Interferon Production Is Mediated by the Double-Stranded RNA-Dependent Protein Kinase PKR
... that PKR serves as a potential PRR and other’s results indi- cating that RIG-I serves as a PRR in WNV-infected cells may be explained in part by the experimental methods employed in these ...virus- ...
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Inhibition of binding to initiation complexes of nascent reovirus mRNA by double-stranded RNA-dependent protein kinase.
... Of necessity, experiments designed to examine whether the binding of nascent reovirus mRNA to initiation complexes was preferentially inhibited in the coupled, cell-free system had to be[r] ...
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Interferon-mediated, double-stranded RNA-dependent protein kinase is inhibited in extracts from vaccinia virus-infected cells.
... Inhibition of the dsRNA-dependent protein kinase activity on exogenous eIF2 in extracts from interferon-treated and control mouse L929 cells infected with vaccinia virus.. Extracts were [r] ...
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RNA-dependent protein kinase (PKR) depletes nutrients, inducing phosphorylation of AMP-activated kinase in lung cancer
... that PKR transfection sometimes induced immunoreactivity at two adjacent bands of lower mobility (Figure ...mutant PKR, or Luc transfection, wild- type PKR transfection showed activated AMPK, with ...
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Transgenes and their contributions to epigenetic research
... In 1990, two publications highlighted that homology-dependent silencing was not restricted to transgenes. Indeed, they demon- strated that an endogenous gene could be switched off when a plant was transformed with ...
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Bluetongue virus VP6 protein binds ATP and exhibits an RNA-dependent ATPase function and a helicase activity that catalyze the unwinding of double-stranded RNA substrates.
... alternative RNA homopolymers poly(A), poly(C), and poly(G) ...nonspecific RNA-binding of VP6 ...the double-stranded homopolymer poly(A) z poly(U) ...9 RNA duplex substrate (lane ...
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In vitro analysis of virus-associated RNA I (VAI RNA): inhibition of the double-stranded RNA-activated protein kinase PKR by VAI RNA mutants correlates with the in vivo phenotype and the structural integrity of the central domain.
... Whether it is a short stem-loop or a large loop in the central domain, our in vitro block assays and the secondary structure analysis suggest that it is this structure that is critical f[r] ...
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Nontemplated Terminal Nucleotidyltransferase Activity of Double-Stranded RNA Bacteriophage φ6 RNA-Dependent RNA Polymerase
... allow RNA molecules to slip into the exit channel ...template-dependent RNA synthesis ...the RNA-dependent RNA polymerization reaction would be mechanistically the same as that ...
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