double-stranded RNA-dependent protein kinase
Prevention of the β-amyloid peptide-induced inflammatory process by inhibition of double-stranded RNA-dependent protein kinase in primary murine mixed co-cultures
... precursor protein; BSA: bovine serum albumin; CNS: central nervous system; COX2: cyclooxygenase 2; DAMPs: danger-associated molecular patterns; DAPI: 4 ’ ,6-diamidino-2- phenylindole; DMSO: dimethylsulfoxide; DTT: ...
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NSs Protein of Rift Valley Fever Virus Induces the Specific Degradation of the Double-Stranded RNA-Dependent Protein Kinase
... viral protein NSs, which was shown to prevent transcriptional induction of the antivirally active type I interferons (alpha/beta interferon [IFN- ␣ /  ...a double-stranded ...
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Cooperative Roles of Fish Protein Kinase Containing Z-DNA Binding Domains and Double-Stranded RNA-Dependent Protein Kinase in Interferon-Mediated Antiviral Response
... dual-specificity kinase (31, ...serine/threonine kinase, IFNs induce ty- rosine phosphorylation of human PKR by activating JAK1 and Tyk2, which are essential for kinase activation and eIF2 ␣ phos- ...
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Functional Domains and the Antiviral Effect of the Double-Stranded RNA-Dependent Protein Kinase PKR from Paralichthys olivaceus
... Ser/Thr protein kinases within the C-terminal region, which contains a KI between subdomains IV and V and a stretch (LYIQMELCD) within subdomain V that is similar to eIF2 ␣ kinase motif LFIQMEFCD ...for ...
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West Nile Virus-Induced Interferon Production Is Mediated by the Double-Stranded RNA-Dependent Protein Kinase PKR
... induced IFN synthesis (consistent with published data [32]), indicating that we had achieved sufficient RIG-I knockdown. One explanation for differences between our data indicating that PKR serves as a potential PRR and ...
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Interferon-mediated, double-stranded RNA-dependent protein kinase is inhibited in extracts from vaccinia virus-infected cells.
... Inhibition of the dsRNA-dependent protein kinase activity on exogenous eIF2 in extracts from interferon-treated and control mouse L929 cells infected with vaccinia virus.. Extracts were [r] ...
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Stress Granules Regulate Double Stranded RNA Dependent Protein Kinase Activation through a Complex Containing G3BP1 and Caprin1
... binding protein (MBP)-tagged MS2 as a control. MS2 is the coat protein from bacteriophage, which is not expressed in mammalian cells, and provides a negative ...
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Inhibition of L-Deleted Foot-and-Mouth Disease Virus Replication by Alpha/Beta Interferon Involves Double-Stranded RNA-Dependent Protein Kinase
... L-deleted FMDV, does not cleave eIF-4G and thus does not shut off host protein synthesis (18). As expected, 2-AP treat- ment had little or no effect on WT FMDV infection. The significance of PKR and/or 2⬘-5⬘A ...
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The E3L and K3L vaccinia virus gene products stimulate translation through inhibition of the double-stranded RNA-dependent protein kinase by different mechanisms.
... This system exploits the ~g unique property of some plasmid-derived mRNAs to elicit localized DAI kinase activation during COS-1 cell transient transfection, resulting in increased eIF-2[r] ...
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Double-stranded RNA-dependent protein kinase and 2-5A system are both activated in interferon-treated, encephalomyocarditis virus-infected HeLa cells.
... The extent of phosphorylation in the intact cell of the a-subunit of eucaryotic protein synthesis initiation factor eIF2 by the kinase was determined for the first time in this type of s[r] ...
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The Dominant-Negative Inhibition of Double-Stranded RNA-Dependent Protein Kinase PKR Increases the Efficacy of Rift Valley Fever Virus MP-12 Vaccine
... mixture of control antibodies (Fig. 6A). RVFV N antigens were not detected in sections derived from mock-vaccinated mice (Fig. 6B) or mice vaccinated with MP-12 or heat-inactivated MP-12, while a few N-positive cells ...
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Human Immunodeficiency Virus Type 1 Tat Protein Activates Transcription Factor NF-κB through the Cellular Interferon-Inducible, Double-Stranded RNA-Dependent Protein Kinase, PKR
... GST protein/15-cm plate (lane 6), or the same amount of the Tat 86 protein (lane 1) and mutant derivatives (lanes 3 to 6), had been delivered by lipofection was used in a gel retardation assay with a ␥- 32 ...
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The Murine Double-Stranded RNA-Dependent Protein Kinase PKR Is Required for Resistance to Vesicular Stomatitis Virus
... Interferons (IFNs) have for some time now been recognized as the cytokines responsible for conferring an antiviral state on cells of the host. These effects are mediated by the gene prod- ucts activated or induced ...
6
Inhibition of binding to initiation complexes of nascent reovirus mRNA by double-stranded RNA-dependent protein kinase.
... Of necessity, experiments designed to examine whether the binding of nascent reovirus mRNA to initiation complexes was preferentially inhibited in the coupled, cell-free system had to be[r] ...
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Toscana Virus NSs Protein Promotes Degradation of Double-Stranded RNA-Dependent Protein Kinase
... All viruses were amplified in VeroE6 cells and then used to infect VeroE6 cells at an MOI of 1. Since PKR is inducible by type I IFNs (28), VeroE6 cells, which lack IFN-␣/ genes (29, 30), were chosen for this experiment ...
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The VP35 Protein of Ebola Virus Inhibits the Antiviral Effect Mediated by Double-Stranded RNA-Dependent Protein Kinase PKR
... PKR is a key component in mediating the interferon-in- duced antiviral response (18). In response to virus infection, PKR forms a homodimer and autophosphorylates on multiple serine, threonine, and tyrosine residues (17, ...
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The Cellular TAR RNA Binding Protein, TRBP, Promotes HIV-1 Replication Primarily by Inhibiting the Activation of Double-Stranded RNA-Dependent Kinase PKR
... TAR RNA into cells has been reported to suppress the efficacy of RNAi silencing ...of RNA isolated from HeLa cells transfected with these plasmids (data not ...
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Herpes Simplex Virus Selectively Induces Expression of the CC Chemokine RANTES/CCL5 in Macrophages through a Mechanism Dependent on PKR and ICP0
... viral RNA is known to be sensed in cells by the double-stranded-RNA-activated protein kinase (PKR) (40), we wanted to test the potential involvement of this kinase in ...
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The C Proteins of Human Parainfluenza Virus Type 1 Limit Double-Stranded RNA Accumulation That Would Otherwise Trigger Activation of MDA5 and Protein Kinase R
... of double-stranded RNA (dsRNA), an intermediate of RNA viral replication, is recognized as a pathogen-associated mo- lecular pattern by Toll-like receptor 3 (TLR3) and two caspase recruitment ...
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The RNA-Binding Protein of a Double-Stranded RNA Virus Acts like a Scaffold Protein
... nonenveloped, double-stranded RNA (dsRNA) virus with a T ⫽ 13 icosahedral capsid, has a virion assembly strategy that initiates with a precursor particle based on an internal scaffold shell similar ...
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