Drosophila testis
Direct control of somatic stem cell proliferation factors by the Drosophila testis stem cell niche
... Niches have traditionally been characterised as signalling microenvironments that allow stem cells to maintain their fate. This definition implicitly assumes that the various niche signals are integrated towards a binary ...
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SUMO regulates somatic cyst stem cell maintenance and directly targets the Hedgehog pathway in adult Drosophila testis
... adult Drosophila testis, both germline stem cells (GSCs) and CySCs interact with a small group of somatic cells termed the hub, which produces major niche signals (de Cuevas and Matunis, 2011; Issigonis and ...
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lines and bowl affect the specification of cyst stem cells and niche cells in the Drosophila testis
... To function properly, tissue-specific stem cells must reside in a niche. The Drosophila testis niche is one of few niches studied in vivo. Here, a single niche, comprising ten hub cells, maintains both ...
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The stem cell niche: lessons from the Drosophila testis
... In the Drosophila testis, GSCs and CySCs are both anchored via an E-cadherin-based mechanism to a fixed population of hub cells. Each GSC is flanked by approximately two CySCs, which results in a ratio of ...
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Hh signalling is essential for somatic stem cell maintenance in the Drosophila testis niche
... the Drosophila testis, germline stem cells (GSCs) and somatic cyst stem cells (CySCs) are arranged around a group of postmitotic somatic cells, termed the hub, which produce a variety of growth factors ...
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A Notch Above Bowl: Specification of Niche Cells in the Drosophila Testis
... the Drosophila testis that genetically manipulate the number of niche cells, modulate the amount of signal produced from the niche cells, or assay changes in the niche in aging flies have confirmed that ...
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Specification and Morphogenesis of the Drosophila Testis Niche
... Adult stem cells have the unique ability to either self-renew or differentiate, thus giving them tremendous therapeutic potential. These tissue-specific stem cells are directed to self-renew by signals from the local ...
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The regulated elimination of transit amplifying cells preserves tissue homeostasis during protein starvation in Drosophila testis
... The Drosophila testis is an ideal model system in which to investigate the behavior of stem cells and transit-amplifying ...In Drosophila male and female germ lines, gametogenesis is highly sensitive ...
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Histone demethylase dUTX antagonizes JAK STAT signaling to maintain proper gene expression and architecture of the Drosophila testis niche
... Mammalian species have multiple H3K27me3-specific demethylases. Therefore, studying the functions of dUTX in Drosophila greatly reduces the complications that might result from gene redundancy. The UTX protein is ...
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Hedgehog is required for CySC self renewal but does not contribute to the GSC niche in the Drosophila testis
... The following antibodies were used: chicken anti--galactosidase (-gal) (1:250; Immunology Consultants Lab), guinea-pig anti-Traffic jam (Tj) (1:3000; gift of D. Godt, University of Toronto, ON, Canada), goat anti- Vasa ...
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Chromatin-Associated Proteins HP1 and Mod(mdg4) Modify Y-Linked Regulatory Variation in the Drosophila Testis
... the Drosophila melanogaster Y chromosome contribute disproportionally to gene expression variation in the ...in Drosophila. Testis-specific YRV is abolished in genotypes with heterozygous ...
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String (Cdc25) regulates stem cell maintenance, proliferation and aging in Drosophila testis
... The Drosophila melanogaster testis contains two stem cell populations that cohere and regulate each other: germline stem cells (GSCs) and cyst stem cells ...the testis apical tip, both GSCs and CySCs ...
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Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning
... The ability to consistently identify CySCs allows us to examine CySC mitoses in detail. We used Ubi-GFP--tubulin to facilitate the analysis of mitotic spindles in GSCs and CySCs. As Ubi Fig. 1. The CySC mitotic spindle ...
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Novel Regulators in the Germline Stem Cell Niche of Drosophila Testis
... In order to generate epitope-tagged UAS-Zfh1, we first needed to know which Zfh1 isoforms were normally expressed in testes. When we began with this approach, two isoforms of Zfh1 had been reported in Drosophila ...
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Gbb/Bmp signaling is essential for maintaining germline stem cells and for repressing bam transcription in the Drosophila testis
... Upd is another known signal for GSCs in the Drosophila testis, and activates the Jak-Stat signal transduction cascade in GSCs to maintain their stem cell identity (Kiger et al., 2001; Tulina and Matunis, ...
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The internal structure of embryonic gonads and testis development in Drosophila melanogaster requires scrib, lgl and dlg activity in the soma
... during Drosophila development, initiated even as soon as the first ever cloned tumour sup- pressor gene in Drosophila, the lethal (2) giant larvae (lgl), had been ...and testis of Drosophila ...
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Live imaging of the Drosophila spermatogonial stem cell niche reveals novel mechanisms regulating germline stem cell output
... the Drosophila testis, GSC maintenance depends on Jak-STAT signaling initiated from the hub, but it is not known whether this same pathway regulates division ...the Drosophila testis will ...
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JAK/STAT signaling in stem cells and regeneration: from Drosophila to vertebrates
... the Drosophila testis, two types of stem cell competition (also termed ‘ niche ’ competition) have been reported: intra-lineage CySC-CySC competition and inter-lineage CySC-GSC ...the testis was ...
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Adhesion in the stem cell niche: biological roles and regulation
... are Drosophila ovarian and testicular stem cells (de Cuevas and Matunis, 2011; Xie, ...the Drosophila testis, GSCs and CySCs precisely orient their spindle apparatus so that the self-renewing stem ...
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THE TESTIS
... of Leydig cell or adrenal cortical origin, promote the growth of the tubules and the development of their lumens. In true precocity all elements of the testis are stimulated, Leydig cell[r] ...
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