Dulbecco's modified Eagle's medium, DMEM
TSG-6 secreted by human adipose tissue-derived mesenchymal stem cells ameliorates severe acute pancreatitis via ER stress downregulation in mice
... in modified PAC maintenance medium containing Dulbecco’s modified Eagle’s medium (DMEM)/ F-12 (Invitrogen), 10% FBS (PAN Biotech), 1% penicillin- streptomycin (PAN Biotech), ...
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Human adipose-derived stem cells inhibit bioactivity of keloid fibroblasts
... Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 mg/mL streptomycin (Gibco, Invitrogen, Carlsbad, CA, USA) at 37 °C in 5% CO 2 ...
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A preliminary evaluation of efficacy and safety of Wharton’s jelly mesenchymal stem cell transplantation in patients with type 2 diabetes mellitus
... ’ s Jelly mesenchymal stem cells With the written consent of the parents, fresh human umbilical cords from both sexes were ...Dulbecco’s modified Eagle’s medium (DMEM) (Gibco Invitrogen, ...
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Injury-induced fetal reprogramming imparts multipotency and reparative properties to pericardial adipose stem cells
... a modified protocol re- ported previously [12], the adipose tissue was washed twice with sterilized phosphate-buffered saline (PBS) and minced into small pieces (about 1×1×1 mm 3 ), and sub- sequently 5 ml of ...
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PDGFRβ+/c-kit+ pulp cells are odontoblastic progenitors capable of producing dentin-like structure in vitro and in vivo
... Pulp tissue from freshly extracted normal human im- pacted third molars (12 subjects, 18–25 years of age) was gently separated from the crown and root, and then digested in 3 mg/ml collagenase type I and 4 mg/ml dis- ...
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Senescent thyrocytes and thyroid tumor cells induce M2-like macrophage polarization of human monocytes via a PGE2-dependent mechanism
... Conditioned medium; CSF-1: Colony-stimulating factor 1; DMEM: Dulbecco ’ s Modified Eagle ’ s Medium; ELISA: Enzyme Linked Immunosorbent Assay; FACS: ...
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Reactive astrocytes undergo M1 microglia/macrohpages-induced necroptosis in spinal cord injury
... Conditioned medium; DMEM: Dulbecco ’ s modified eagle ’ s medium; dpi: Days post-injury; EM: Electronic microscopic; FBS: Fetal bovine serum; GdCl3: Gadolinium ...
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Antioxidant-upregulated mesenchymal stem cells reduce inflammation and improve fatty liver disease in diet-induced obesity
... obese; DMEM: Dulbecco ’ s modified Eagle ’ s medium; ELISA: Enzyme- linked immunosorbent assay; FBS: Fetal bovine serum; GFP: Green fluorescent protein; GOI: Gene of ...
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Ectopic expression of TWIST1 upregulates the stemness marker OCT4 in the esophageal squamous cell carcinoma cell line KYSE30
... cells; DMEM: Dulbecco ’ s Modified Eagle ’ s medium; EMT: Epithelial – mesenchymal transition; ESCC: Esophageal squamous cell carcinoma; ETS2: ETS proto- oncogene 2; FBS: ...
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ACE2 inhibits breast cancer angiogenesis via suppressing the VEGFa/VEGFR2/ERK pathway
... DMEM: Dulbecco ’ s modified Eagle ’ s medium; ECM: Endothelial cell medium;; EMT: Epithelial-to-mesenchymal transition; FBS: Foetal bovine serum;; GEO: Gene ...
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Extracellular vesicles derived from human Wharton’s jelly mesenchymal stem cells protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers
... Dulbecco’s modified Eagle’s medium F-12 (DMEM F-12; Invitrogen) with 1% antibiotics, digested with type II collagenase (200 U/mL) for 16 h at 37 °C under slow agitation, and submitted to a 2000×g ...
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Intra-carotid arterial transfusion of circulatory-derived autologous endothelial progenitor cells in rodent after ischemic stroke—evaluating the impact of therapeutic time points on prognostic outcomes
... volume; DMEM: Dulbecco ’ s modified Eagle ’ s medium; ECL: Enhanced chemiluminescence; EPC: Endothelial progenitor cells; FBS: Fetal bovine serum; GFAP: Glial fibrillary ...
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MicroRNA-495 confers inhibitory effects on cancer stem cells in oral squamous cell carcinoma through the HOXC6-mediated TGF-β signaling pathway
... genes; DMEM: Dulbecco ’ s modified Eagle ’ s medium; DMSO: Dimethyl sulfoxide; EGF: Epithelial growth factor; EMT: Epithelial- mesenchymal transition; FBS: Fetal bovine ...
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Human amniotic fluid stem cell injection therapy for urethral sphincter regeneration in an animal model
... conditioned medium; CP: closing pressure; DAPI: 4,6-diamidino-2-phenylindole 2HCl staining; DMEM: (Dulbecco ’ s) Modified Eagle ’ s Medium; FACS: ...
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Is exclusion of leukocytes from platelet-rich plasma (PRP) a better choice for early intervertebral disc regeneration?
... Dulbecco’s modified Eagle’s medium with low glucose (DMEM-LG, Gibco, Waltham, MA, USA) for 4–6 ...in DMEM-LG supplemented with 10% FBS, 100 U/mL penicillin, 100 mg/mL ...
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Intravenous multipotent adult progenitor cell therapy after traumatic brain injury: modulation of the resident microglia population
... Dulbecco’s modified Eagle’s/F12 medium with GlutaMAX (DMEM/ F12) supplemented with 10% FBS, 100units/ml penicil- lin, 100 g/ml streptomycin and 5 ng/ml of granulocyte macrophage colony stimulating ...
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Human adult mesangiogenic progenitor cells reveal an early angiogenic potential, which is lost after mesengenic differentiation
... Cells showed MSC-like spindle-shaped morphology (Fig. 1e) and expressed mesenchymal markers STRO-1, CD73, and CD90 while MPC-associated markers be- came undetectable (Fig. 1f ). P2-MSCs revealed F-actin reorganization in ...
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Different sensitivity of germinal center B cell-like diffuse large B cell lymphoma cells towards ibrutinib treatment
... with the specially designed primers and probes and Plat- inum Quantitative PCR SuerMix-UDG (Cat No.11730- 017, Life Technologies, Carlsbad, CA, USA). The primers and probes were as follows: Btk sense CCGGAAGA- ...
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Exosomes secreted by stem cells from human exfoliated deciduous teeth contribute to functional recovery after traumatic brain injury by shifting microglia M1/M2 polarization in rats
... TERT-SHED were cultured in DMEM with exosome- free FBS (SBI, Palo Alto, CA, USA) for 48 h. The cell culture media was then collected and mixed with the ExoQuick exosome isolation solution, and the following steps ...
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Harvesting multipotent progenitor cells from a small sample of tonsillar biopsy for clinical applications
... a small sample of tonsillar tissue. We have successfully generated T-MPC cell lines from 14 donors (9 females and 5 males). These samples were distributed across three age groups: pediatric (age 3–12 years; n = 7), young ...
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