E. coli BL21 (DE3)
Batch production of a silk-elastin-like protein in E. coli BL21(DE3): key parameters for optimisation
... in E. coli by use of the pET25b-E. coli BL21 (DE3) expression system, with yields of approximately 150 mg/L being reported ...
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Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)
... Results: T. fusca cutinase was fused with the specific signal peptide of alpha-hemolysin scretion system and expressed in E. coli BL21(DE3). In addition, HlyB and HlyD, strain-specific ...
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High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG
... the E. coli integral membrane chaperone YidC and the E. coli glutamate proton symporter GltP in BL21(DE3) cells cultured in lysogeny broth (LB medium), we made an unexpected ...
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Activation of formate hydrogen-lyase via expression of uptake [NiFe]-hydrogenase in Escherichia coli BL21(DE3)
... Escherichia coli (hydrogenase-1) in E. coli BL21(DE3), a strain that lacks H 2 -evolving ...wild-type BL21(DE3), the recombinant BL21(DE3) strains possessed ...
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Production of the short peptide surfactant DAMP4 from glucose or sucrose in high cell density cultures of Escherichia coli BL21(DE3)
... titer, E. coli BL21(DE3) pEDA was grown at low cell density in a rich complex medium consisting of LB medium supplemented with glucose ...
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Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of l-DOPA from d-glucose
... and plasmid extractions. Primers used in this study are listed in Additional file 1: Table S1. Target genes (hpaBC, galP, glk, ppsA, and tktA) were amplified from E. coli BL21 (DE3) genome. ...
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False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid-Based Western Blot Assay Were Rectified by the Use of Two Subunits (S1 and S2) of Spike for Detection of Antibody to SARS-CoV
... Escherichia coli strain BL21(DE3) (Novagen, Merck Eurolab, Fontenay Sous Bois, ...transformed BL21(DE3) host cells were incubated for 6 to 8 h at 37°C in 4 ml of Luria-Bertani (LB) ...
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Batch, Fed Batch Production and Characterization of Glutaminase Free L Asparaginase II of Pectobacterium carotovorum MTCC 1428 in Escherichia coli
... in E. coli B), Type II L-asparaginase has higher affinity towards asparagine than Type I L-aspa- ...in E. coli BL21 (DE3) in ...
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Identification of new glutamate decarboxylases from Streptomyces for efficient production of γ-aminobutyric acid in engineered Escherichia coli
... Streptomyces is rich in natural products with various biological activities, such as antibacterial, antiviral, anti-cholesterol, antiprotozoan and antitumor properties [13]. As a result, Streptomyces is well studied for ...
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Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration
... The ORF TM0438 was confirmed to encode a hyperther- mophilic 6PGDH. Through different approaches (expres- sion vectors, hosts, cultivation conditions, inducer type, and gene sequence), we increased expression levels of ...
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Microbial production of lipid-protein vesicles using enveloped bacteriophage phi6
... in E. coli thin sections The phi6 major envelope protein P9 and the non-struc- tural protein P12 are the minimum requirements for viral envelope formation in the Pseudomonas host ...in E. coli ...
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Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli
... Escherichia coli is commonly used in academia and industry for expressing recombinant proteins because of its well-characterized molecular genetics and the availability of numerous expression vectors and ...15 ...
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Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway
... interest. E. coli BL21(DE3) or similar hosts with the DE3 lysogen and well defined plasmid vectors are widely used because they make it possible to precisely balance the expression of ...
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Co-expression of active human cytochrome P450 1A2 and cytochrome P450 reductase on the cell surface of Escherichia coli
... medium. E. coli BL21(DE3) is able to completely synthesize heme from glutamate via 5-ALA in the cytosol and to transport it to the periplasm ...
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A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3)
... has been found to be specific to l-Phe, with an activ- ity of up to 35.7 IU/g [27, 28]. In the present study, to improve expression level, codon-optimized versions of all the above-mentioned genes were synthesized. FDC1 ...
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Molecular cloning and host optimization study for enhanced expression of thermostable protease to meet the commercial demand
... The E. coli BL21 (DE3), E. coli C41 (DE3) and rosetta were used parallel and separately for expression analysis under IPTG ...
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High Yield Overexpression, Refolding, Purification and Characterization of Pseudomonas aeruginosa Type B-Flagellin: An Improved Method Without Sonication
... The immunoreactivity of purified r-B-flagellin under modified conditions was examined in vitro by ELISA, opsonophagocytosis and motility inhibition assays. The high titer of polyclonal antibodies was detected by ELISA ...
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Immunogenicity assay of KatG protein from Mycobacterium tuberculosis in mice: preliminary screening of TB vaccine
... escherichia coli BL21 с ис- пользованием вектора ...в e. coli BL21 получали протеин KatG с моле- кулярной массой 80 кДа при электрофорезе на ...
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Glucose uptake regulation in E. coli by the small RNA SgrS: comparative analysis of E. coli K-12 (JM109 and MG1655) and E. coli B (BL21)
... by E. coli B (BL21) and E. coli K-12 (JM109) strengthens the hypothesis that the difference in the acetate excretion between these two strains is related to the difference in glucose ...
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E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)
... with BL21(DE3) we knew that E. coli needs approximately 4 h of adap- tion before being able to take up lactose at its maximum uptake rate ...
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