E1B-55K
The E3 Ubiquitin Ligase Activity Associated with the Adenoviral E1B-55K–E4orf6 Complex Does Not Require CRM1-Dependent Export
... of E1B-55K and E4orf6 to replication centers and to cytoplasmic foci has been related to their activities in selective viral late mRNA metabolism and in the assembly of the Cul5-containing E3 ubiquitin ...
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Adenovirus E4 34k and E1b 55k Oncoproteins Target Host DNA Ligase IV for Proteasomal Degradation
... 34k/E1b 55k complex constitutes the substrate-binding compo- nent of a viral E3 ubiquitin ligase also containing cellular cullin 5, elongins B and C, and Rbx1 that mediates proteasomal degradation of Mre11 ...
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Analysis of the Adenovirus E1B-55K-Anchored Proteome Reveals Its Link to Ubiquitination Machinery
... The E1B-55K complex in Ad5-infected cells polyubiquiti- nates p53 in ...The E1B-55K and E4-orf6 proteins are required to counter the E1A-induced stabilization of p53 in both virus-infected and ...
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KAP1 Is a Host Restriction Factor That Promotes Human Adenovirus E1B-55K SUMO Modification
... Plasmids and transient transfections. The HAdV proteins examined in this study were expressed from their respective complementary DNAs under the control of the cytomegalovirus immediate early promoter de- rived from the ...
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Proteasome-Dependent Degradation of Daxx by the Viral E1B-55K Protein in Human Adenovirus-Infected Cells
... lines, E1B-55K protein is diffusely distributed in wild-type-infected HepaRG cells ...59). E1B-55K forms some distinct cytoplasmic aggregates and localizes in a limited number of globular con- ...
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p53-Independent and -Dependent Requirements for E1B-55K in Adenovirus Type 5 Replication
... eIF-4E phosphorylation is also reduced late in adenovirus infection (42). The subsequent impaired activity of eIF-4F has been proposed to shut off the synthesis of host proteins (42, 89). The translation of adenovirus ...
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E4Orf6-E1B-55k-Dependent Degradation of De Novo-Generated Adeno-Associated Virus Type 5 Rep52 and Capsid Proteins Employs a Cullin 5-Containing E3 Ligase Complex
... E1A, E1B-55k, E2A, and VA RNA) required for efficient adeno-associated virus (AAV) virus pro- duction (2, 4, ...with E1B-55k, has been shown to cause the ubiquitinylation of cellular p53 and ...
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Analyses of Single-Amino-Acid Substitution Mutants of Adenovirus Type 5 E1B-55K Protein
... The E1B-55K protein plays an important role during human adenovirus type 5 productive ...infection, E1B-55K binds to and inactivates the tumor suppressor protein p53, allowing efficient ...
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E4orf1 Limits the Oncolytic Potential of the E1B-55K Deletion Mutant Adenovirus
... the E1B-55K-encoded protein to promote viral late gene ...the E1B-55K- and E4orf6-encoded proteins promote p53 degradation (51, ...the E1B-19K- encoded protein, which promotes cell ...
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E1B-55K-Mediated Regulation of RNF4 SUMO-Targeted Ubiquitin Ligase Promotes Human Adenovirus Gene Expression
... an E1B-55K null mutant (H5pm4149, right) at a multiplicity of 50 FFU per cell and harvested after indicated time points ...an E1B-55K null mutant (H5pm4149, right) at a multiplicity of 50 FFU ...
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RUNX1 Permits E4orf6-Directed Nuclear Localization of the Adenovirus E1B-55K Protein and Associates with Centers of Viral DNA and RNA Synthesis
... The E1B-55K protein is a multifunctional protein with a complex distribution throughout the nucleus and cytoplasm of the adenovirus-infected ...of E1B-55K is affected by interactions with ...
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Inhibition of p53 by Adenovirus Type 12 E1B-55K Deregulates Cell Cycle Control and Sensitizes Tumor Cells to Genotoxic Agents
... Alternatively, E1B-55K might be able to trigger a DNA- damaging signal, indirectly leading to posttranslational modi- fications of p53 and its ...Ad12 E1B-55K plays a key role in inducing ...
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Adenovirus E4-ORF3 Targets PIAS3 and Together with E1B-55K Remodels SUMO Interactions in the Nucleus and at Virus Genome Replication Domains
... of E1B-55K/E4- ORF3. These data demonstrate that E1B-55K and E4-ORF3 have novel roles in determining the structure and nuclear compart- mentalization of E2A viral replication ...(62). ...
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The Adenovirus Type 5 E1B-55K Oncoprotein Actively Shuttles in Virus-Infected Cells, Whereas Transport of E4orf6 Is Mediated by a CRM1-Independent Mechanism
... ported a only partial inhibition of E4orf6 shuttling by LMB in heterokaryon assays (23). Although we did not observe this inhibition in our assay 1 h after fusion, this report supports our observations on E4orf6 export. ...
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Adenovirus E1B 55K Represses p53 Activation In Vitro
... In contrast to our knowledge of activators, relatively little is known regarding the mechanisms utilized by repressors. Re- pressors can be divided into classes: global and specific. Global repressors, such as Dr1/DRAP ...
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The Adenovirus E1B 55-Kilodalton and E4 Open Reading Frame 6 Proteins Limit Phosphorylation of eIF2α during the Late Phase of Infection
... the E1B-55K and E4orf6 proteins and their ability to form the Cul5-based E3 ubiquitin-protein ligase ...the E1B-55K or E4orf6 mutant viruses ap- peared to be mediated by PKR, which itself was ...
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Adenovirus E4orf3 Targets Transcriptional Intermediary Factor 1γ for Proteasome-Dependent Degradation during Infection
... proteins, E1B-55K and E4orf6, to usurp control of cellular ubiquitin ligases and target pro- teins for proteasome-dependent degradation during infection is well ...of E1B-55K and E4orf6, ...
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Adenovirus Regulates Sumoylation of Mre11-Rad50-Nbs1 Components through a Paralog-Specific Mechanism
... (Ad5) E1B-55K and E4-open reading frame 6 (ORF6) proteins form an E3 ubiquitin (Ub) ligase complex with cellular proteins cullin 5 (CUL5), Rbx1, and elongins B and C (24, 42) and inacti- vate the MRN ...
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Adenovirus E1B 55-Kilodalton Protein Is a p53-SUMO1 E3 Ligase That Represses p53 and Stimulates Its Nuclear Export through Interactions with Promyelocytic Leukemia Nuclear Bodies
... of E1B- 55K and p53 sumoylation to contribute to the tethering of unsumoylated p53 in ...Since E1B-55K is a dimer (54) that binds to the random coil N termini of p53 tetramers (39, 40, 50), it ...
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A 49-Kilodalton Isoform of the Adenovirus Type 5 Early Region 1B 55-Kilodalton Protein Is Sufficient To Support Virus Replication
... before, E1B-55K was seen with MAb 2A6 in lysates from wt- but not from H5pm4133-infected ...The E1B-55K protein was also de- tected with MAb 4E8 in wt H5pg4100-infected cells ...Ad5 ...
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