ecto-5'-nucleotidase
Synthesis of Silver Nanoparticles from Malva parviflora Extract and Effect on Ecto-5'- Nucleotidase(5'-NT), ADA and AMPDA Enzymes in Sera of Patients with Arthrosclerosis Ahlam M. Farhan| Rasha A. Jassim| Nafeesa J. Kadhim| Wesen Adel Mehdi | Atheer Awad Mehde
... and Ecto-5'-nucleotidase ...and Ecto-5'-nucleotidase ...method[7]. Ecto-5'-nucleotidase activity was measured in serum according to Wood and Williams's ...
9
Ecto 5′ nucleotidase (CD73) regulation by hypoxia inducible factor 1 mediates permeability changes in intestinal epithelia
... surface-expressed ecto-5 ′ -nucleotidase (CD73) and subsequently activates surface adenosine recep- tors regulating endothelial and epithelial barrier ...and ecto-apyrase CD39 in hypoxic ...
11
Ecto 5′ Nucleotidase Activity in Human T Cell Subsets DECREASED NUMBERS OF ECTO 5′ NUCLEOTIDASE POSITIVE CELLS FROM BOTH OKT4+ AND OKT8+ CELLS IN PATIENTS WITH HYPOGAMMAGLOBULINEMIA
... T lymphocytes from control subjects were separated into subsets using monoclonal antibodies of the OKT series and complement lysis and analyzed for ecto-5¢-nucleotidase activity both by quantitative ...
9
Characterization of recombinant mouse ecto-5'-nucleotidase
... Given that two subtypes of adenosine activated purinergic receptors were localized to the spinal cord (Choca et al., 1987; Geiger et al., 1984; Goodman and Synder, 1982), the investiga[r] ...
110
Insights into thymic purine metabolism and adenosine deaminase deficiency revealed by transgenic mice overexpressing ecto 5' nucleotidase (CD73)
... that 5 9-NT sub- strates are available in the thymus, but adenosine fails to accu- mulate because it is metabolized to inosine either directly via ADA or indirectly via adenosine kinase, AMP deaminase, and ...
9
Methotrexate and sulfasalazine promote adenosine release by a mechanism that requires ecto 5' nucleotidase mediated conversion of adenine nucleotides
... the ecto-5 9 -nucleotidase–mediated dephosphorylation of adenine nucleotides ...of ecto-5 9 -nucleotidase in- creases adenine nucleotide and decreases adenosine concen- trations ...
7
Synthesis of immunoglobulin G by pokeweed mitogen or Epstein Barr virus stimulated human B cells in vitro is restricted to the ecto 5' nucleotidase positive subset
... lymphocytes because its expression increases during normal development and is reduced in many patients with B cell immunodeficiencies. To determine whether this enzyme defines functional subsets of B lymphocytes, human ...
5
Ecto-5′-nucleotidase (CD73) attenuates inflammation after spinal cord injury by promoting macrophages/microglia M2 polarization in mice
... A RIPA buffer (25 mM Tris•HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) was used to extract total protein for subsequent concentration analysis with the BCA assay. SDS-polyacrylamide gel ...
14
Characterization and properties of ecto-5' nucleotidase in white adipose tissue
... 5 'Nase in a 0 . 5M sodium bicarbonate buffer, pH 9 . A and left overnight for maximal binding. Unbound protein was removed the next day by at least three washes with a 0.9%(w/v) NaCl, 0.1%(v/v) Tween 20 solution. ...
219
Impairment of tubuloglomerular feedback regulation of GFR in ecto 5′ nucleotidase/CD73–deficient mice
... The main observation in the present study is that mice with a null mutation in the e-5′NT/CD73 gene have a markedly reduced ability to translate changes in luminal NaCl concentration into changes in glomerular ...
10
Comparison of the efficacy and safety of FP 1201 lyo (intravenously administered recombinant human interferon beta 1a) and placebo in the treatment of patients with moderate or severe acute respiratory distress syndrome: study protocol for a randomized controlled trial
... surface ecto-5 ′ -nucleotidase; COPD: Chronic obstructive pulmonary disease; CPAP: Continuous positive airway pressure; CT: Computed tomography; FAS: Full analysis set; HFOV: High-frequency ...
9
Acidosis Is a Key Regulator of Osteoblast Ecto-Nucleotidase Pyrophosphatase/Phosphodiesterase 1 (NPP1) Expression and Activity
... phosphatase > ecto-5-nucleotidase > Enpp3 > NTPdase 1 > NTPdase 3 > Enpp2 > NTPdase 2. Acidosis (pH 6.9) upregulated NPP1 mRNA (2.8-fold) and protein expression at all stages of ...
8
5' adenosine monophosphate is the neutrophil derived paracrine factor that elicits chloride secretion from T84 intestinal epithelial cell monolayers
... Additional studies suggested that neutrophil-derived 5'-AMP is subsequently converted to adenosine at the epithelial cell surface by ecto-5'-nucleotidase and that adenosine subsequently [r] ...
7
Mechanism of nitrogen metabolism-related parameters and enzyme activities in the pathophysiology of autism
... that 5’-NT activity is increased in response to abnormal accumulation of some nucleotide substrates ...in 5’-NT activity in patients with autism com- pared with control subjects might be related to the sig- ...
11
Bacterial UDP Glucose Hydrolases and P2 Receptor Mediated Responses to Infection: A Commentary
... for 5’- and other nucleotides, can be considered to be the degradation of external nucleotides enabling the uptake and use of nu- cleosides and phosphate ...
5
Evaluation of in vitro anti snake venom activity of Justicia adhatoda leaves extract against Russell’s viper snake venom
... like 5' nucleotidase, L-amino acid oxidase, phosphomonoesterase, phosphodiesterase, acetylcholine esterase and hyaluronidase from the Russell’s viper snake ...
7
Elevated 5' nucleotidase and alkaline phosphodiesterase activities in trout gill localize to endothelial (pillar) cells
... of 5′NT and APD in fish gill are quite ...including 5′NT, in trout gill tissue. 5′NT is a biochemically well-characterized protein, with the exoenzyme attached to the plasma membrane via a ...
9
Regulatory processes in the central nervous system: A study of g-protein abundance and of soluble 5'-nucleotidase
... It is generally appreciated that at least four distinct isoforms of 5'-nucleotidase exist (see Zimmerman, 1992 for review). One form is an ectoenzyme which is believed to facilitate production of ...
235
Cathepsin Cleavage Potentiates the Ebola Virus Glycoprotein To Undergo a Subsequent Fusion-Relevant Conformational Change
... Other factors required for fusion triggering. A surprising finding of our study is that mild reduction is sufficient to trigger the 19-kDa GP Ecto to bind to liposomes (Fig. 7) and to inactivate the ability of ...
9
Adenosine formed by 5′ nucleotidase mediates tubuloglomerular feedback
... Nephron function is stabilized by tubuloglomerular feedback (TGF). TGF operates within the jux- taglomerular apparatus, sensing changes in tubular flow and eliciting compensatory changes in sin- gle nephron GFR (SNGFR). ...
11