EHEC O157:H7
Core 2 mucin-type O-glycan inhibits EPEC or EHEC O157:H7 invasion into HT-29 epithelial cells
... or EHEC O157:H7 could invade into HT-29, HT-29-OBN, HT-29-Gal, or HT- 29-Gal-OBN cells, bacterial invasion experiments using GFP-labeled EPEC or EHEC O157:H7 in HT-29-Gal, ...
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Lessons learned from a textbook outbreak: EHEC-O157:H7 infections associated with the consumption of raw meat products, June 2012, Limburg, Belgium
... We described a foodborne outbreak, involving 24 cases of which 5 developed HUS, in which the source could be traced back to a slaughterhouse. PFGE and IS629- printing proved that beef products, sold at different ...
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Comparison of clinical and immunological findings in gnotobiotic piglets infected with Escherichia coli O104:H4 outbreak strain and EHEC O157:H7
... is indicative for inflammation. In this study haptoglobin concentrations were significantly increased in E. coli O157:H7 infected animals. This can be interpreted as an early sign of inflammation with or ...
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Distribution of the Urease Gene Cluster among and Urease Activities of Enterohemorrhagic Escherichia coli O157 Isolates from Humans
... (EHEC) O157:H7 strain EDL933 (27) and the Sakai O157:H7 outbreak strain (13) demonstrated two and one loci, respectively, consisting of seven urease genes (ureD, -A, -B, -C, -E, -F, and ...
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Distribution of tccP in Clinical Enterohemorrhagic and Enteropathogenic Escherichia coli Isolates
... in EHEC O157:H7, have been ...in O157:H7, in non-O157 EHEC, and in typical and atypical EPEC ...the EHEC O157:H7 strains tested were tccP ⴙ ...
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Development of a Gold Nanoparticle Vaccine against Enterohemorrhagic Escherichia coli O157:H7
... Two EHEC-specific immunogenic antigens, namely, LomW and EscC, either alone or in combination, were covalently linked on the surface of gold nanoparticles (AuNPs) and used to immunize mice prior to challenge with ...
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Structure and Function of Plasmid pColD157 of Enterohemorrhagic Escherichia coli O157 and Its Distribution among Strains from Patients with Diarrhea and Hemolytic Uremic Syndrome
... (EHEC) O157:H7 strain CL40cu, was characterized by restriction mapping and determination of its complete nucle- otide ...of EHEC O157:H7 isolates from patients with diarrhea or ...
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Molecular Characterization of Human Atypical Sorbitol Fermenting Enteropathogenic Escherichia coli O157 Reveals High Diversity
... (EHEC) O157:H7, serogroup O157 comprises sorbitol- fermenting typical and atypical enteropathogenic ...eae-positive EHEC serotypes and the composition of the rfbO157 ...with EHEC ...
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Identification of epitopes recognised by mucosal CD4+ T-cell populations from cattle experimentally colonised with Escherichia coli O157:H7
... (EHEC) O157:H7 causes diarrhoea and potentially fatal renal failure in humans as a result of Shiga toxin (Stx) activity ...for EHEC O157:H7 [2] and this has led to the ...
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Molecular typing of enterohemorrhagic Escherichia coli O157:H7 isolates in Japan by using pulsed field gel electrophoresis
... (EHEC) O157:H7 isolates, most of which were from 19 outbreaks and 608 sporadic cases in Japan, mainly in May to August ...the EHEC O157:H7 isolates were classified into six types ...
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Detection of toxB, a Plasmid Virulence Gene of Escherichia coli O157, in Enterohemorrhagic and Enteropathogenic E coli
... PCR analyses. The primer pair toxB.911F/toxB.1468R described by Tarr and colleagues (30) was used to amplify a fragment corresponding to the 5⬘ region of toxB, under the conditions described by the authors. Primer pairs ...
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Data-driven network modelling of disease transmission using complete population movement data: spread of VTEC O157 in Swedish cattle
... The following simulation experiment was conducted to explore the VTEC O157 spread model on a national scale. In each of the eight NUTS level 2 regions in Swe- den, a set of 126 holdings were randomly selected. In ...
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Serotypes, Virulence Genes, and Intimin Types of Shiga Toxin (Verotoxin) Producing Escherichia coli Isolates from Human Patients: Prevalence in Lugo, Spain, from 1992 through 1999
... STEC O157:H7 to ferment sorbitol to improve ...STEC O157:H7, although our results as well as other findings prove otherwise (13, ...STEC O157:H7-positive specimens did not ...
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Escherichia coli O157:H7 and O157:H−Strains That Do Not Produce Shiga Toxin: Phenotypic and Genetic Characterization of Isolates Associated with Diarrhea and Hemolytic Uremic Syndrome
... coli O157 LPS. Therefore, the presence of E. coli O157 was ...coli O157:H7 Rapid Latex Test Kit revealed that these colonies were serotype O157:H ⫺ ...
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Isolation of Shiga toxin-producing Escherichia coli from raw milk in Kermanshah, Iran.
... In summary, our data revealed that non-O157:H7 STEC are more prevalent, and the high presence of STEC in raw milk confirms the important role of raw milk as putative vehicle of infection to human. Moreover, ...
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Incidence and Virulence Determinants of Verocytotoxin Producing Escherichia coli Infections in the Brussels Capital Region, Belgium, in 2008–2010
... VTEC O157:H⫺ isolates positive for vtx1a plus vtx2c recovered from two bloody diarrhea patients in August ...of O157 strains that had spread in the ...VTEC O157:H7 iso- lates carrying vtx1a ...
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Shiga Toxin Producing Escherichia coli in Children: Diagnosis and Clinical Manifestations of O157:H7 and Non O157:H7 Infection
... Premier EHEC assay resulted in more false-positive results than SMAC culture and therefore had a lower positive predictive value ...Premier EHEC assay at CHB, 9 were positive in the Premier EHEC ...
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A rapid and highly sensitive protocol for the detection of <em>Escherichia coli</em> O157:H7 based on immunochromatography assay combined with the enrichment technique of immunomagnetic nanoparticles
... bands of heavy- and light-chain Ig. Strip material is also closely related to experimental results. Two NC membranes of different pore size and flow rate were compared and the HF135 NC membrane from EMD Millipore was ...
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Detection of Nonspecific Binding of E. coli O157:H7 on Reduced Graphene Oxide Screen-Printed Carbon Electrodes Using Electrochemical Methods
... coli O157:H7; IgG; nonspecific binding; reduced graphene oxide; linear sweep.. 28.[r] ...
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Sorbitol Fermenting Shiga Toxin Producing Escherichia coli O157:H− Strains: Epidemiology, Phenotypic and Molecular Characteristics, and Microbiological Diagnosis
... coli O157 using standard biochemical tests (2) and agglutination with anti- O157 serum ...STEC O157 infection in the Czech Republic (7, 8) combines a direct culture or the IMS-enriched culture on ...
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