ELISPOT, enzyme-linked immunospot
A phase II trial of autologous dendritic cell vaccination and radiochemotherapy following fluorescence guided surgery in newly diagnosed glioblastoma patients
... Abbreviations 5-ALA: 5-aminolevulinic; CR: complete radiological resection; DC: dendritic cell; ELISA: enzyme-linked immunosorbent assay; ELISPOT: enzyme-linked immunospot assay; FGS: fl[r] ...
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Selected RD1 Peptides for Active Tuberculosis Diagnosis: Comparison of a Gamma Interferon Whole-Blood Enzyme-Linked Immunosorbent Assay and an Enzyme-Linked Immunospot Assay
... than ELISPOT (81% versus 71%), although the difference was not statistically ...RD1 ELISPOT in children with active pulmonary TB (18). The fact that ELISPOT and whole-blood assays correlate well has ...
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Memory T-Cell Response to Rotavirus Detected with a Gamma Interferon Enzyme-Linked Immunospot Assay
... interferon enzyme-linked immuno- spot (ELISPOT) assay for evaluation of CMI responses to rotavirus using frozen PBMCs obtained from healthy ...interferon ELISPOT responses were similar for CCA ...
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Recombinant Mycobacterium bovis BCG Expressing the Chimeric Protein of Antigen 85B and ESAT-6 Enhances the Th1 Cell-Mediated Response
... IFN- ␥ ELISPOT assay. The mice were euthanized, and their spleens were removed aseptically. The spleens were gently ground through a 70-m cell strainer, and then single-cell suspensions were prepared by ...
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Enzyme-Linked Immunospot Assays Provide a Sensitive Tool for Detection of Cytokine Secretion by Monocytes
... bation period, therefore, are likely to influence the assay re- sults and ease of discriminating spot from background staining considerably. Optimal incubation of ELISPOT plates, there- fore, should result in high ...
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Comparison of Enzyme-Linked Immunospot Assay and Tuberculin Skin Test in Healthy Children Exposed to Mycobacterium tuberculosis
... where the numbers of individuals are represented by rectangles of a size that correlate with their relative proportion. Sixty (26.7%) of the TST-positive children were ELISPOT negative, and 55 (25%) who had a pos- ...
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Diagnosis of Active Tuberculosis in China Using an In-House Gamma Interferon Enzyme-Linked Immunospot Assay
... the ELISPOT assay (but not the TST) with TB exposure suggests that these ELISPOT-positive healthy controls were subjected to ...homemade ELISPOT assay is much lower than the overall prevalence ...
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Varicella-Zoster Virus-Specific Enzyme-Linked Immunospot Assay Responses and Zoster-Associated Pain in Herpes Zoster Subjects
... ) enzyme-linked immunospot (ELISPOT) ...␥ ELISPOT responses to VZV antigen than did HC (n ⴝ 140). ELISPOT geometric mean count (GMC) responses (with 95% confidence intervals ...
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Evaluation of the Interlaboratory Concordance in Quantification of Human Immunodeficiency Virus-Specific T Cells with a Gamma Interferon Enzyme-Linked Immunospot Assay
... The ELISPOT plate material has been shown to cause some dif- ferences ...automatic ELISPOT reader system was identical, but each laboratory used its own ...
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Enzyme-Linked Immunospot Assay Detection of Mumps-Specific Antibody-Secreting B Cells as an Alternative Method of Laboratory Diagnosis
... Statistical analysis. Receiver operating characteristic (ROC) analysis was performed with Prism software (GraphPad Software Inc., La Jolla, CA) to estimate the sensitivity and specificity of the ELISPOT test and ...
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Rotavirus-specific intestinal immune response in mice assessed by enzyme-linked immunospot assay and intestinal fragment culture.
... Oral inoculation of mice with WC3 induced low frequencies of virus-specific IgA-secreting cells by ELISPOT assay; this find- ing correlated with the small quantities of IgA secreted by G[r] ...
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Use of Enzyme Linked Immunospot Assay with Mycobacterium tuberculosis Specific Peptides for Diagnosis of Recent Infection with M tuberculosis after Accidental Laboratory Exposure
... an ELISPOT assay using ...on ELISPOT, two lab- oratory technicians with the highest level of exposure during the accident were found to respond strongly to ESAT-6 and ...with ELISPOT 1 year after the ...
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A T cell based assay using specific Mycobacterium tuberculosis antigens, ESAT-6 and CFP 10, for the diagnosis of Tuberculous Meningitis.
... ex-vivo enzyme linked immunospot (ELISPOT) assay for ...the immunospot assay, sensitization to one or more antigens specific to only ...
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Asian Elephant T Cell Responses to Elephant Endotheliotropic Herpesvirus
... ␥ enzyme-linked immunospot (ELISpot) assay as a means to assess protective T cell immune responses in elephants, we first focused on rabies as a model pathogen and specifically on measuring ...
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Human Immunodeficiency Virus-Specific CD8+ T Cells in Human Breast Milk
... (IFN-␥) enzyme-linked immunospot (ELISPOT) assay was used to enumerate HIV-specific T cells from breast milk or peripheral blood mononuclear cells (PBMC) of HIV-infected or uninfected women ...
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Analysis of Virus-Specific CD4+ T Cells during Long-Term Gammaherpesvirus Infection
... The IFN-␥ enzyme-linked immunospot ELISpot assay 5, 9 was used to screen peptide response profiles for CD4⫹ T cells enriched from the spleens of The murine gammaherpesvirus 68 ␥HV68 prov[r] ...
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Diagnosis of Central Nervous System Tuberculosis by T-Cell-Based Assays on Peripheral Blood and Cerebrospinal Fluid Mononuclear Cells
... tuberculosis-specific enzyme-linked immunospot (ELISPOT) assay with samples from the site of infection may permit a more sensitive or specific diagnosis of active central nervous system (CNS) ...
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Increase in circulating Th17 cells during anti TNF therapy is associated with ultrasonographic improvement of synovitis in rheumatoid arthritis
... by enzyme-linked immunospot assay (Elispot) after anti-TNF treatment in patients with anky- losing spondylitis, psoriatic arthritis and RA ...
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Potential Role of Immunodiagnosis for Pulmonary Tuberculosis Using Induced Sputum Cells
... an enzyme-linked immunospot assay using induced sputum cells (IS ELISPOT) for a rapid diagnosis of pulmonary tuberculosis ...rolled. ELISPOT assay was performed using cells from induced ...
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Anti HER2 CD4+ T helper type 1 response is a novel immune correlate to pathologic response following neoadjuvant therapy in HER2 positive breast cancer
... γ enzyme- linked immunosorbent spot (ELISPOT) assay ...γ ELISPOT results from a single representative pathological complete response (pCR) (left panel) and non-pCR (right panel) patients' ...
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