Elongation factor 2 kinase
FOXM1 regulates expression of eukaryotic elongation factor 2 kinase and promotes proliferation, invasion and tumorgenesis of human triple negative breast cancer cells
... Eukaryotic elongation factor 2 kinase (eEF2K), an emerging molecular target for cancer therapy, contributes to cancer proliferation, cell survival, tumorigenesis, and invasion, disease ...
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Activity of translation regulator eukaryotic elongation factor-2 kinase is increased in Parkinson disease brain and its inhibition reduces alpha synuclein toxicity
... eukaryotic elongation factor-2 kinase (eEF2K) mediates critical regulation of dendritic mRNA translation and is a crucial molecule in diverse forms of synaptic ...
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Everolimus enhances cellular cytotoxicity of lapatinib via the eukaryotic elongation factor-2 kinase pathway in nasopharyngeal carcinoma cells
... Notes: (A) cne-2 and hOne-1 cells were treated with lapatinib (0-5 μM) or DMsO control for 48 hours. Phospho-egFr, egFr, phospho-her2, and her2 were examined by western blotting. gaPDh was used as a loading ...
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The Ability of Positive Transcription Elongation Factor b To Transactivate Human Immunodeficiency Virus Transcription Depends on a Functional Kinase Domain, Cyclin T1, and Tat
... FIG. 3. Squelching assays revealed functional interactions between CDK9 and cyclin T1 in cells. The expression of free CDK9 reduced effects of the hybrid RevCDK9 protein on SLIIB (15- to 3-fold transactivation [lanes ...
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Eukaryotic Elongation Factor 1δ Is Hyperphosphorylated by the Protein Kinase Encoded by the UL13 Gene of Herpes Simplex Virus 1
... (iii) Viruses have evolved a variety of mechanisms to mod- ulate the host cell translational apparatus to aid in their rep- lication. In most cases reported so far, viruses have targeted proteins designed to maintain the ...
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Conserved Protein Kinases Encoded by Herpesviruses and Cellular Protein Kinase cdc2 Target the Same Phosphorylation Site in Eukaryotic Elongation Factor 1δ
... 1, 2, 5, and 6), whereas another mutant, EF-1␦:S133A(F), was not posttranslationally processed in mock-infected or infected cells (lanes 3 and ...protein kinase(s) in uninfected cells and by the viral ...
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Eukaryotic elongation factor 2 controls TNF α translation in LPS induced hepatitis
... Previous reports have identified p38α as a key kinase involved in TNF-α production (43). p38α MAPK deficiency in macrophages results in decreased TNF-α production, with a modest effect on IL-6 expression (44). ...
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Elevated eukaryotic elongation factor 2 expression is involved in proliferation and invasion of lung squamous cell carcinoma
... EF2 kinase, which is a dedicated kinase for which EF2 is the only known substrate and binding to the ribosome is prevented, then protein synthesis is subsequently inhibited ...
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Involvement of nucleoside diphosphate kinase b and elongation factor 2 in Leishmania braziliensis antimony resistance phenotype
... translation elongation, but EF2 along EF1A are one of the most important enzymes which conduct the elongation cycle of protein synthesis in eukaryotic cells ...the elongation of essential proteins ...
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Purification and characterization of elongation factor 2 (EF2) and cyclic AMP-independent protein kinases from soybean (Glycine max, L) cotyledons.
... Step 1 ; V Separation of protein kinase by DEAE-cellulose Chromatography Post-ribosomal supernatant (prepared as outlined in Chapter II) was made 6 mM with B-mercaptoethanol. The pH of the sample was adjusted to ...
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Helicobacter pylori-controlled c-Abl localization promotes cell migration and limits apoptosis
... cell elongation and scattering ...protein kinase A inhibitor (PKI) used as a negative control, Gö6983 slightly affected phos- phorylation of pAbl T735 , while bis(indolyl)maleimide (BIM) drastically reduced ...
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The cytotoxic effect of diphtheria toxin on the actin cytoskeleton
... Diphtheria toxin (DT) is a well-characterised bacterial toxin, both in terms of its structure and its mode of action [1]. DT consists of a 535 amino acid polypeptide and contains the following three domains: the amino ...
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Proteostatic Signaling & Control of Protein Synthesis
... for binding transmembrane mitochondrial outer membrane proteins that fail to be inserted into the mitochondrial membrane and delivers them to the proteasome [115]. Since the loss of ubiquilins results in the cytosolic ...
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Structural Basis for EarP Mediated Arginine Glycosylation of Translation Elongation Factor EF P
... translation elongation factors EF-P and IF-5A is usually achieved by posttranslational elongation of the -amino group of a conserved lysine (20–23, 48, ...
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Ribosomal Elongation Factor 4 Promotes Cell Death Associated with Lethal Stress
... Support for the dual function of EF4 comes from two direc- tions. First, EF4 allows translation to restart when EF-G mal- functions (3). Such activity is expected to contribute to the recovery of cells from moderate ...
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Aberrations in translational regulation are associated with poor prognosis in hormone receptor positive breast cancer
... of 2-methylimidazo- lium iodide has been identified as a potent and relatively specific inhibitor of EF2K; it was shown to have in vitro anticancer activity against cell lines of a variety of tumor lineages ...
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The action of ricin A chain on eukaryotic ribosomes
... gradient buffer: 10 m M Tria/HCl, pH 7.5, 70 m M NH^Cl and 4 oM Mg(0Ac>2« Hie gradients were made using a Densiflow II gradient maker ( Buchler Instrunents ). Reticulocyte lysate translation mixtures were ...
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The myogenic kinome: protein kinases critical to mammalian skeletal myogenesis
... cyclin kinase inhibitor p21, triggering its dissociation from CDK2 and leading to cell cycle pro- gression ...transcription factor and inhibiting expression of FoxO1-regulated transcripts such as the CDK ...
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In silico identification of EGFR-T790M inhibitors with novel scaffolds: start with extraction of common features
... As described above, common features of currently known irreversible TKIs were extracted (Figures 1–3). Commonly, each of those TKIs has an acrylamide group or a propargyl amide group. At the same time, they all bear one ...
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New developments in the treatment of HER2-positive breast cancer
... Another potential benefit of lapatinib may be in the set- ting of metastatic disease to the brain. HER2-positive breast cancers have an increased tendency to metastasize to the brain, possibly because of increased ...
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