embryonic cell nuclear transfer
Application of new biotechnologies for improvements in swine nutrition and pork production
... as embryonic cell nuclear transfer ...of embryonic cells as a ...somatic cell nuclear transfer ...the transfer of a nucleus from a donor cell into a ...
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Improvement of mouse cloning using nuclear transfer derived embryonic stem cells and/or histone deacetylase inhibitor
... somatic cell nuclear transfer is still low and in this study, we could obtain cloned mice from four individuals out of seven donor ...ntES cell lines from all individuals and healthy cloned ...
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The influence of interspecies somatic cell nuclear transfer on epigenetic enzymes transcription in early embryos
... somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic ...the nuclear transfer failure ...
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Nuclear Transfer of Embryonic Cell Nuclei to Non-enucleated Eggs in Zebrafish, Danio rerio
... for nuclear transfer in zebrafish were the same as those described for medaka by Bubenshchikova et ...donor cell was then slightly ruptured by suction into a glass microcapil- lary tube (inner ...
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Recipient cell nuclear factors are required for reprogramming by nuclear transfer
... by nuclear transfer has demonstrated that the differentiated state of a cell can be reprogrammed to a more embryonic character and that developmental potential can be fully restored (Gurdon, ...
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Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors
... improve embryonic development is supported by studies analysing the effect of the genetic distance between the donor cell and the recipient ...donor cell and recipient oocyte mtDNA populations result ...
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Molecular conditions of the cell nucleus remodelling/reprogramming process and nuclear transferred embryo development in the intraooplasmic karyoplast injection technique: a review
... the nuclear transfer (NT) embryos at 1-cell stage are very often known as pseudopronuclei or ap- parent pronuclei as well as spurious pronuclei and presumptive/pretended ...as nuclear envelope ...
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Amphibian interorder nuclear transfer embryos reveal conserved embryonic gene transcription, but deficient DNA replication or chromosome segregation
... Fig. 7. Embryonic gene tran- scription in interorder cybrid embryos. RNA was extracted from embryos of the kinds and stages indicated, and subjected to RT- qPCR analysis for selected genes using P. waltl-specific ...
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Somatic cell nuclear transfer: failures, successes and the challenges ahead
... somatic cell to the cytoplasm of the ...donor cell is lysed by repeated aspiration and ejection with a ...increase nuclear reprogramming ...Sertoli cell (Ogura et ...2001a), embryonic ...
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Generation of bovine transgenics using somatic cell nuclear transfer
... Embryonic stem (ES) cells are used extensively as way to create transgenic mice. These cells are isolated from the inner cell mass of a blastocyst and, kept in the right culture conditions, have the ...
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Gene transfer technologies leading to transgenic animals
... the cell from an external source is called ...gene transfer technologies leading to transgenic ...DNA transfer to anmals by microinjection, biolistics and ...to embryonic stem cell ...
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Serum starvation-induced cell cycle synchronization stimulated mouse rDNA transcription reactivation during somatic cell reprogramming into iPSCs
... somatic cell reprogramming to pluripotency [15, ...human embryonic stem cells ...upon nuclear transfer, a nu- clear reprogramming ...Practically, embryonic stem cell ...
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Nuclear transfer: Progress and quandaries
... assessing embryonic developmental potential is genomic ...the nuclear transfer procedure, and the synchronization of donors and ...recipients. Cell Cycle Coordination and NT ...
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Choice of random rather than imprinted X inactivation in female embryonic stem cell derived extra embryonic cells
... ES cell in vitro differentiation system indicated that female ES cells carrying two active X chromosomes lost all the imprinted marks on the paternal and maternal X chromosomes that might otherwise direct ...
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Mechanosensing By The Nuclear Lamina: From Embryonic Development To Aging
... of cell function and ...beating embryonic heart, following a mechanism for tension-suppressed turnover that confers mechano-protection against DNA ...increased nuclear envelope rupture, ii) ...
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Progress toward generating a ferret model of cystic fibrosis by somatic cell nuclear transfer
... adoptive transfer into pseudopregnant recipi- ent ...embryo transfer in the ferret and open the door to animal modeling with this species following embryo manipulation ...
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Transfer reactions for nuclear astrophysics
... The calculation of the transfer reaction cross sections with DWBA formalism involves the evalua- tion of a six dimensional integral [1]. For light-ion reactions such as (d,p) reaction, the direct reaction causing ...
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Adenoviral delivery of E2F 1 directs cell cycle reentry and p53 independent apoptosis in postmitotic adult myocardium in vivo
... the cell cycle precludes the ability of cardiac muscle cells to increase cell number after ...exogenous cell cycle regulators has not been tested, in vitro or in ...gene transfer of E2F-1 ...
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Development of genome engineering technologies in cattle: from random to specific
... embryo transfer into a recipient was performed because of the ease of the ...somatic cell nuclear transfer (SCNT) was introduced and successfully used to produce cloned ...
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Generation of functional salivary gland tissue from human submandibular gland stem/progenitor cells
... During natural submandibular gland development, after the invagination of the oral epithelium into mesen- chyme at E11, the mesenchyme begins to condense and an early initial epithelial bud generates at E12. A branch- ...
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