Engineering a disulfide bond into the E9 DNase
Engineering de novo disulfide bond in bacterial alpha-type carbonic anhydrase for thermostable carbon sequestration
... novo disulfide bond in a bacterial α-type CA to enhance ...novo disulfide engineering can be used as an efficient biocatalyst for carbon sequestration under high temperature ...
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Disulfide by Design 2.0: a web-based tool for disulfide engineering in proteins
... potential disulfide bonds in the aforementioned 331 non-homologous PDB ...one disulfide bond; 2) less than 50% sequence identity; and 3) structural resolution ...for bond predic- tions; ...
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Disulfide by Design 2.0: a web-based tool for disulfide engineering in proteins
... potential disulfide bonds in the aforementioned 331 non-homologous PDB ...one disulfide bond; 2) less than 50% sequence identity; and 3) structural resolution ...for bond predic- tions; ...
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A Prediction Method of Protein Disulfide Bond Based on Hybrid Strategy
... structures, Disulfide bonds to maintain a stable three-dimensional structure of the protein and to remain active and function of the protein has a very im- portant ...of disulfide bond is a key step ...
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The roles of surface loop insertions and disulfide bond in the stabilization of thermophilic WF146 protease
... disulfide bond, resembling its psychrophilic (subtilisins S41 and S39) and mesophilic (subtilisins SSII and sphericase) ...fide bond played a critical role in stabilizing the WF146 protease against ...
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Removal of a Conserved Disulfide Bond Does Not Compromise Mechanical Stability of a VHH Antibody Complex
... and Engineering, ETH Zurich, 4058 Basel, Switzerland * S Supporting Information ABSTRACT: Single-domain VHH antibodies are promising reagents for medical ...fide bond within the VHH framework region is known ...
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Topological Plasticity of Enzymes Involved in Disulfide Bond Formation Allows Catalysis in Either the Periplasm Or the Cytoplasm
... enzymes disulfide bond form- ing enzyme B (DsbB) and vitamin K epoxide reductase (VKOR) are central to oxidative protein folding in the periplasm of ...structural disulfide bonds in proteins also ...
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Protein disulfide engineering
... disulfide engineering applications While the goal of most disulfide engineering experiments has focused on improving protein stability, the introduction of novel disulfides can be an effective tool in a range ...
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Disulfide Bond Configuration of Human Cytomegalovirus Glycoprotein B
... of disulfide bonds of CMV ...The disulfide bond configuration of HSV-2 gB is indicated by solid lines connecting appropriate cysteine ...the disulfide bond configuration of CMV gB ...
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A Structural Model of Pestivirus Erns Based on Disulfide Bond Connectivity and Homology Modeling Reveals an Extremely Rare Vicinal Disulfide
... a disulfide-linked homodimer of 100 kDa; it is found on the surface of pestivirus-infected cells and is secreted into the ...the disulfide arrangement of the nine cysteines present in the mature dimer was ...
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Quality control of disulfide bond formation in pilus subunits by the chaperone FimC
... structural disulfide bond between Cys228 and Cys233 in the P pilus chaperone PapD would no longer form in the absence of DsbA, thereby preventing PapD folding and thus pilus biogenesis 23 ...that ...
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Carbohydrates Facilitate Correct Disulfide Bond Formation and Folding of Rotavirus VP7
... The fact that PDI only interacted with correctly N-linked glycosylated VP7 suggests that carbohydrates and PDI are closely associated with correct folding of the ER-resident VP7. The exact role of the N-linked ...
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Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin.
... RESULTS Disulfide bond formation and folding of the HCV glycopro- ...monitor disulfide bond formation in viral glycoproteins ...of disulfide bonds and often reflects progressive folding ...
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The Fusion-Controlling Disulfide Bond Isomerase in Retrovirus Env Is Triggered by Protein Destabilization
... DISCUSSION According to the present model for MLV Env induced mem- brane fusion, binding of the SU subunit to the cell receptor results in isomerization of the SU-TM disulfide bond, SU dis- sociation, and ...
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Evolution of the HIV 1 envelope glycoproteins with a disulfide bond between gp120 and gp41
... An intriguing question is why the loss of the SOS disulfide bond occurred in multiple independent cultures via a sub- stitution of C605, but never of C501. This is a surprising finding given the fact that a ...
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Cysteine sulfenic Acid as an Intermediate in Disulfide Bond Formation and Nonenzymatic Protein Folding
... of disulfide bond formation at any given point in ...four disulfide bonds was unambiguously provided (see Figure S3 of the Supporting Information for a series of illustrative mass ...last ...
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Disulfide bond reconstruction: A novel approach for grafting of thiolated chitosan onto wool
... the disulfide bonds in the wool fiber during the reduction pretreatment with L-cysteine, resulting in the degradation of the wool ...reform disulfide bond within the ...
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Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli
... the disulfide bond-dependent proteins will be briefly ...dependent disulfide bond formation ...the disulfide bonds are formed in the presence of a suitable redox sys- tem ...maximize ...
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Enhanced production of a single domain antibody with an engineered stabilizing extra disulfide bond
... extra disulfide had similar affinity for the L1 antigen as L1-G2+ and main- tained over 90 % of its antigen binding ability up to its melting temperature of ~80 ...
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Baculovirus GP64 Disulfide Bonds: the Intermolecular Disulfide Bond of Autographa californica Multicapsid Nucleopolyhedrovirus GP64 Is Not Essential for Membrane Fusion and Virion Budding
... intersubunit disulfide bond, resulting in isomer- ization and formation of a disulfide between the two cysteines of the CXXC ...more disulfide bonds in cell surface-localized F protein of ...
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