enhanced green fluorescent protein
Development of a novel Newcastle disease virus (NDV) neutralization test based on recombinant NDV expressing enhanced green fluorescent protein
... Observer.A1 fluorescent microscope (Carl Zeiss, Germany). The fluorescent signal images were taken at 400X magnification with the AxioCam MRc5 camera (Carl Zeiss, ...
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Generation of a Recombinant Akabane Virus Expressing Enhanced Green Fluorescent Protein
... FIG 1 (A) Schematic depiction of negative-sense (AKAV) and ambisense (RVFV) gene expression strategies. AKAV, with a negative-sense strand-coding strategy, possesses an S RNA segment that contains a single ...
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Attenuation of Simian Varicella Virus Infection by Enhanced Green Fluorescent Protein in Rhesus Macaques
... show green fluorescence and immunohistochemical staining (using anti-SVV antiserum) of plaques associated with cytopathic effects in Vero cells and rhesus fibroblasts infected with SVV recombinant C or ...
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Efficient reduction of synthetic mRNA induced immune activation by simultaneous delivery of B18R encoding mRNA
... recombinant protein B18R is often required to avoid IFN ...of enhanced green fluorescent protein (eGFP) mRNA transfected fibroblasts with B18R encoding mRNA over 7-days resulted in ...
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Time resolved studies of energy transfer in thin films of green and red fluorescent proteins
... derived fluorescent proteins are attractive candidates for lasing and sens- ing due to their excellent optical properties, including their high quantum yield, spec- tral tunability and robustness against ...
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Identification of Six Autographa californica Multicapsid Nucleopolyhedrovirus Early Genes That Mediate Nuclear Localization of G-Actin
... express enhanced green fluorescent protein–Bombyx mori G-actin in transient transfection experiments, we identified six AcMNPV early genes that mediate nuclear localization of G-actin in ...
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Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatant
... We are developing retroviral-mediated gene transfer to human fibroblast-like synovial cells (FLS) as one approach to characterizing genetic pathways involved in synoviocyte pathophysiology. Prior work has suggested that ...
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<p>Lipo-PEG-PEI complex as an intracellular transporter for protein therapeutics</p>
... and enhanced green fluorescent protein (EGFP) as model proteins to examine the transport process of ...vivo protein delivery, we intravenously injected LPPC- β G s into BALB/c mice and ...
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Identification of the Lymantria disparNucleopolyhedrovirus Envelope Fusion Protein Provides Evidence for a Phylogenetic Division of the Baculoviridae
... An enhanced green fluorescent protein (EGFP) expression plasmid was constructed by first cloning a 717-bp BamHI- NotI fragment containing the egfp gene from pEGFP-N1 (Clontech, ...a ...
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Use of the viral 2A peptide for bicistronic expression in transgenic mice
... a fluorescent reporter of 2A function We designed a strategy to enable us to assess visually 2A function using fluorescent proteins localised to two dis- tinct cellular ...localised enhanced ...
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The Hinge of the Human Papillomavirus Type 11 E2 Protein Contains Major Determinants for Nuclear Localization and Nuclear Matrix Association
... E2 protein of papillomaviruses is a site-specific DNA binding nuclear ...recognition protein and assists in the assembly of the preinitiation ...E2 protein consists of an amino-terminal (N) ...
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Non-invasive evaluation of embryo morphological plasticity by designing of new transgenic gene cassette
... novel enhanced green fluorescent protein (EGFP) gene cassette to eliminate some consume time in livestock industry to assay high quality embryos and morphological ...
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Real-Time In Vivo Green Fluorescent Protein Imaging of a Murine Leishmaniasis Model as a New Tool for Leishmania Vaccine and Drug Discovery
... with enhanced green fluorescent protein, enabling in vivo real-time whole-body fluorescence imaging, to follow the progression of Leishmania infection in parasitized ...
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Conditional gene expression systems in the transgenic rat brain
... and enhanced green fluorescent protein (EGFP) under the control of the bidirectional tet-inducible promoter (Ptetbi, [21]) (EGFP-Ptetbi-luc line; Figure ...
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Potential utility of eGFP-expressing NOG mice (NOG-EGFP) as a high purity cancer sampling system
... of enhanced green fluorescent protein (eGFP) were useful for the clear separation of tumor cells and mouse stromal cells in subcutaneous xenografted tumors by fluorescence activated cell ...
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Differential regulation of blood flow induced neovascularisation and mural cell recruitment by VEGF and angiopoietin signaling
... form in the mesentery (Wang et al. 2004). Ad.Ang1 and Ad.eGFP (enhanced green fluorescent protein) were a gift from Regeneron Inc., Tarrytown, NY, USA (Benest et al. 2006) and Ad.eNOS ...
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Interaction of Poxvirus Intracellular Mature Virion Proteins with the TPR Domain of Kinesin Light Chain in Live Infected Cells Revealed by Two-Photon-Induced Fluorescence Resonance Energy Transfer Fluorescence Lifetime Imaging Microscopy
... Construction of plasmids. A plasmid that encodes enhanced green fluorescent protein was constructed in two steps. Briefly, the DNA spanning the coding sequence of EGFP was amplified from ...
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Generation of Replication-Competent Recombinant Influenza A Viruses Carrying a Reporter Gene Harbored in the Neuraminidase Segment
... coding enhanced green fluorescent protein (EGFP) or lucifer- ase, could be of great use in studying influenza virus and developing vaccines and antiviral ...glyco- protein of the ...
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Proteolytic Processing of Human Cytomegalovirus Glycoprotein B Is Dispensable for Viral Growth in Culture
... encoding enhanced green fluorescent protein was constructed, and complementation by mutant gBs was initially evaluated in transient-cotransfection ...of green fluorescent ...
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High frequency, cell type-specific visualization of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants
... red fluorescent protein; EGFP or just G: enhanced green fluorescent protein; GFP: green fluorescent protein; R: DsRed2; mRed or mR: DsRed-monomer ...
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