enzyme linked fluorescence assay
Comparison of enzyme linked immunosorbent assay with enzyme linked fluorescence assay with automated readers for detection of rubella virus antibody and herpes simplex virus
... The enzyme-linked immunosorbent assay ELISA was compared with the enzyme-linked fluorescence assay ELFA for the detection of rubella antibody and herpes simplex virus antigen.. Test para[r] ...
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Enzyme linked fluorescence assay: Ultrasensitive solid phase assay for detection of human rotavirus
... In addition, the sensitivity of the rotaviral detection systems was established by testing logio dilutions in quadruplicate of a standard filtrate prepared from the stool of a gnotobioti[r] ...
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A phase II trial of autologous dendritic cell vaccination and radiochemotherapy following fluorescence guided surgery in newly diagnosed glioblastoma patients
... Abbreviations 5-ALA: 5-aminolevulinic; CR: complete radiological resection; DC: dendritic cell; ELISA: enzyme-linked immunosorbent assay; ELISPOT: enzyme-linked immunospot assay; FGS: fl[r] ...
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Pitfalls and fallacies of cat scratch disease serology: evaluation of Bartonella henselae based indirect fluorescence assay and enzyme linked immunoassay
... During the last few years it has become clear that the ma- jority of cat scratch disease (CSD) cases are caused by Bar- tonella henselae (2, 6, 10, 13, 19). Culture of B. henselae from the lymph nodes of CSD patients has ...
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Direct comparison of the histidine rich protein 2 enzyme linked immunosorbent assay (HRP 2 ELISA) and malaria SYBR green I fluorescence (MSF) drug sensitivity tests in Plasmodium falciparum reference clones and fresh ex vivo field isolates from Cambodia
... MSF assay, for analysing fresh clinical isolates of relatively low ...Green fluorescence I is expected to improve MSF assay ...and assay sensitivity for low parasitaemia samples ...MSF ...
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Treatment of malignant effusion by oncolytic virotherapy in an experimental subcutaneous xenograft model of lung cancer
... ELISA: Enzyme-linked immunosorbent assay; FACS: Fluorescence-activated cell sorting; FBS: Fetal bovine serum; Gd-DTPA: Gadopentetate-Dimeglumine; GFP: Green fluorescent protein; iv: ...
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Overexpressing TGF-β1 in mesenchymal stem cells attenuates organ dysfunction during CLP-induced septic mice by reducing macrophage-driven inflammation
... Fig. the MSC-NC group). the MSC-NC group). ELISA, enzyme-linked immunosorbent assay; MFI, mean fluorescence intensity; MSCs, mesenchymal stem cells; MSC-NC, mesenchymal stem cell carryin[r] ...
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Evaluation of a Commercial Glycoprotein Enzyme Linked Immunosorbent Assay for Measuring Vaccine Immunity to Varicella
... no fluorescence or non-spe- cific ...weak fluorescence with partial ring, 1+ showed specific clear fluorescence with complete thin ring structure, 2+ showed bright and specific fluorescence ...
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Association of an overlap syndrome of autoimmune hepatitis and primary biliary cirrhosis with cytomegalovirus infection
... Tests for hepatitis A IgM antibody, hepatitis B surface antigen, and anti-hepatitis C virus antibody were negative. ANA was negative by fluorescence assay (, × 40), but it was positive as measured using ...
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Enzyme linked immunosorbent fluorescence assay and high pressure liquid chromatography for analysis of humoral immune responses to Coxiella burnetti proteins
... A microtiter enzyme-linked immunosorbent fluorescence assay based on alkaline phosphatase conjugate and 4-methylumbelliferyl phosphate as fluorogenic substrate was developed and adapted [r] ...
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Drying anti malarial drugs in vitro tests to outsource SYBR green assays
... Green assay offers a rapid and cheap alternative to other in vitro assays, but it requires tools not commonly available in field ...green fluorescence assay performed at a specialized laboratory ...
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Development of a novel peptide aptamer-based immunoassay to detect Zika virus in serum and urine
... Interestingly, use of a symmetric pair of peptides to detect ZIKV in a sandwich assay did not interfere with use of the peptide pair for capturing and detection. This is likely attributable to less competition ...
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Comparison of a Classical Phagocytosis Assay and a Flow Cytometry Assay for Assessment of the Phagocytic Capacity of Sera from Adults Vaccinated with a Pneumococcal Conjugate Vaccine
... Flow assay. The flow cytometry phagocytosis assay was performed as described (14). Pneumococcal strains (serotype 6A, 6B, 19F, and 23F) (American Type Culture Collection [Manassas, Va.] strains), kindly ...
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Evaluation of enzyme linked immunosorbent assay for the serodiagnosis of amebiasis
... When the results of ELISA were compared with those of the IFA test performed on a total of 514 sera from patients with clinically proven and suspected amebiasis, positive findings were 3[r] ...
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Adaptation of enzyme linked immunosorbent assay to the avian system
... 499 198 60 NAd NA NA The plastic surfaces, treated or untreated with FCS, as described in the text, were compared for their capacity to bind nonspecifically either normal chicken sera SP[r] ...
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Enzyme linked immunosorbent assay for detection of measles antibody
... Detection of er, the mean value for a particular titer was IgG antibodies for measles virus by enzyme-linked immunosorbent assay ELISA.. This variation is due in part to the discrete two[r] ...
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Enzyme linked immunosorbent assay for Clostridium difficile toxin A
... difficile strains by the ELISA for the presence of toxin A, and our results demonstrated that the concentration of toxin A by ELISA closely correlated with the cytotoxic titers of toxins[r] ...
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Enzyme linked immunosorbent assay for the diagnosis of cerebral cysticercosis
... To evaluate the reliability of ELISA for the diagnosis of central nervous system CNS cysticercosis, sensitivity, specificity, positive and negative predictive values were calculated for [r] ...
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A poxvirus-encoded uracil DNA glycosylase is essential for virus viability.
... Ethidium bromide fluorescence assay of the refolded SFV uracil DNA glycosylase using uracil-containing plasmid DNA as a substrate.. Following treatment with the enzyme, the fluorescence [r] ...
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Surface immunofluorescence assay for diagnosis of Lyme disease
... A surface immunofluorescence assay SIFA was analyzed and compared with a conventional indirect immunofluorescence assay IFA and whole-cell enzyme-linked immunosorbent assay ELISA for det[r] ...
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