enzyme-linked immunosorbent analysis
Enzyme linked immunosorbent assay using recombinant envelope protein 2 antigen for diagnosis of Chikungunya virus
... The sensitivity and specificity of rE2-CHIKV ELISA, EIA-ICC and immunochromatographic test for IgM de- tection were estimated by the Bayesian method intro- duced by Joseph et al. [44]. The Bayesian method allows us to ...
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Changes in salivary oxidative status, salivary cortisol, and clinical symptoms in female patients with temporomandibular disorders during occlusal splint therapy: a 3-month follow up
... ANOVA: Repeated-measurements analysis of variance; CPI: Characteristic pain intensity; DD: Disc displacement; ELISA: Enzyme-linked immunosorbent assay; HIP: High intensity pain; LIP: Low[r] ...
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Expression of Escherichia coli heat labile enterotoxin B subunit in transgenic tomato (Solanum lycopersicum L ) fruit
... blot analysis showed that only two of the five T 0 transgenic tomato plants expressed the pentameric LTB protein in the ...An enzyme-linked immunosorbent assay indicated that these two plants ...
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Serological Response of Shiga Toxin-Producing Escherichia coli Type III Secreted Proteins in Sera from Vaccinated Rabbits, Naturally Infected Cattle, and Humans
... blot analysis and enzyme- linked immunosorbent assay (ELISA) using sera from rabbits immunized with type III secreted proteins (T3SPs) from four STEC serotypes, experimentally infected cattle, ...
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Molecular Basis of the Interaction between Complement Receptor Type 2 (CR2/CD21) and Epstein-Barr Virus Glycoprotein gp350
... an enzyme-linked immunosorbent assay (ELISA) for the efficient analysis of the gp350-CR2 interaction by utilizing wild-type and mutant forms of recombinant gp350 and also of the CR2 N-terminal ...
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Analysis of nonspecific reactions in enzyme linked immunosorbent assay testing for human rotavirus
... The ELISA assay for human rotavirus was performed as previously described with goat anti-rotavirus antiserum used to coat the wells of a polyvinyl microtiter plate Dynatech 220-24 and, a[r] ...
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Enzyme-linked immunosorbent assay of nicotine metabolites
... by enzyme- linked immunosorbent assay (ELISA) and by chromatography–mass spectrometry ...after analysis of variance (ANOVA) using logarithmic ...logarithmic analysis, P \ ...
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Evaluation of a Novel Aspergillus Antigen Enzyme Linked Immunosorbent Assay
... The latest generation of the LFD was CE marked in 2018. The novel JF5-based GP ELISA will be CE marked in 2019 and will be the first commercially available Aspergillus antigen ELISA since the launch of the Platelia kit ...
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Evaluation of a Commercial Glycoprotein Enzyme Linked Immunosorbent Assay for Measuring Vaccine Immunity to Varicella
... The coefficients of variation (CVs) of ten sera for the in- tra-assay assessment were between 2.0-18.1%, and all sam- ples, except one, were within the permitted range (≤15%) of manufacturer’s instructions (Table 1). An ...
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Development and evaluation of enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for diagnosis of invasive aspergillosis
... our experiment. Further more, a potential risk of con- tamination with such non-target microbes nucleic acids could be ignored because only the target nucleic acids with T7 promoter can be amplified. Thus, the hybridi- ...
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Antibody-sandwich ELISA analysis of a novel blood biomarker of CST4 in gastrointestinal cancers
... An antibody overlap test was performed to analyze the epitopes of antibodies. Each epitope respectively recog- nized by the 5D2F2, 5E4G5, 2G7A9, 3F4G8, and 4F1B6 monoclonal antibodies was confirmed. The results (Figure ...
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Effectiveness of obstetrical interventions and quality of labor on neonatal viability in dogs
... Enzyme Linked Immunosorbent Assay (ELISA). The influence of type of whelping and duration of labor on the incidence of stillborn births and incidence of mortality [r] ...
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Tumor expression, plasma levels and genetic polymorphisms of the coagulation inhibitor TFPI are associated with clinicopathological parameters and survival in breast cancer, in contrast to the coagulation initiator TF
... RNA isolation and TFPI and TF mRNA expression in tumor Total RNA was isolated from the breast tumors with Trizol and quality controlled using the Agilent 2100 Bioanalyzer. 100 ng total RNA was applied to the Sure- Print ...
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Enzyme linked immunosorbent assay for the diagnosis of cerebral cysticercosis
... To evaluate the reliability of ELISA for the diagnosis of central nervous system CNS cysticercosis, sensitivity, specificity, positive and negative predictive values were calculated for [r] ...
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Enzyme linked immunosorbent assay for streptococcal M protein antibodies
... Protein fraction a from type M-12, group A streptococcal cells elicits bactericidal antibodies in rabbits and has opsonic inhibitory activity 21.. Therefore, fraction a was used in the E[r] ...
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Enzyme Linked Immunosorbent Assay for Immunological Diagnosis of Human Tularemia
... tularensis LPS and ratio of relative IgGI IgM titers of sera obtained from healthy controls and from patients with tularemia in the acute phase of disease and 2.5 years after infection N[r] ...
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Enzyme linked immunosorbent assay for detection of Mycoplasma pneumoniae antibodies
... Determination of the optimal antigen concentrations was done using varied dilutions of antigen 1.5 to 48 lig of proteins per well, a positive reference serum diluted 1:16, and conjugated[r] ...
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Comparative accuracy and 'field friendly' effectiveness of diagnostic tools for lymphatic filariasis and neurocyticercosis in Papua New Guinea and Timor-Leste with consideration on the impact of parasitic reduction programs
... The study could have been improved by the research team spending more time involved with the survey at the collection sites, but budget constraints prevented this. The sensitivity of the parasitology methods could have ...
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Evaluation of enzyme linked immunosorbent assay for the serodiagnosis of amebiasis
... When the results of ELISA were compared with those of the IFA test performed on a total of 514 sera from patients with clinically proven and suspected amebiasis, positive findings were 3[r] ...
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Enzyme linked immunosorbent assay for Clostridium difficile toxin A
... difficile strains by the ELISA for the presence of toxin A, and our results demonstrated that the concentration of toxin A by ELISA closely correlated with the cytotoxic titers of toxins[r] ...
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