enzyme-linked immunospot
Enzyme-Linked Immunospot Assays Provide a Sensitive Tool for Detection of Cytokine Secretion by Monocytes
... Blood monocytes as well as tissue-differentiated macrophages play a pivotal role in controlling immune reactions. Monocytes regulate the extent, nature, and duration of immune responses by secretion of cytokines. ...
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Comparison of Enzyme-Linked Immunospot Assay and Tuberculin Skin Test in Healthy Children Exposed to Mycobacterium tuberculosis
... Large-scale evaluation of enzyme-linked immunospot assay and skin test for the diag- nosis of Mycobacterium tuberculosis infection against a gradient of exposure in the Gambia. Clin Infe[r] ...
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Varicella-Zoster Virus-Specific Enzyme-Linked Immunospot Assay Responses and Zoster-Associated Pain in Herpes Zoster Subjects
... ] enzyme-linked immunospot [ELISPOT] assays) (10, 21), whereas the level of serum immuno- globulin antibody to VZV remains relatively constant with age (10, 15, ...
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Evaluation of the Interlaboratory Concordance in Quantification of Human Immunodeficiency Virus-Specific T Cells with a Gamma Interferon Enzyme-Linked Immunospot Assay
... Efficient clinical trials to evaluate vaccination or immunity- based therapeutic strategies aimed at inducing human immu- nodeficiency virus (HIV)-specific T cells require rapid, accu- rate, and reliable methods for ...
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Detection of Human Papillomavirus Type 16-Specific T Lymphocytes by a Recombinant Vaccinia Virus-Based Enzyme-Linked Immunospot Assay
... Cell-mediated immunity, particularly that induced by T cells, is thought to have a key role in controlling infection. The enzyme-linked immunospot (ELISPOT) assay has been successfully adapted to ...
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Selected RD1 Peptides for Active Tuberculosis Diagnosis: Comparison of a Gamma Interferon Whole-Blood Enzyme-Linked Immunosorbent Assay and an Enzyme-Linked Immunospot Assay
... ) enzyme-linked immunospot assay (ELISPOT), using selected early secreted antigenic target 6 (ESAT-6) peptides, that appears specific for active tuberculosis ...whole-blood ...
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Optimal Blood Mononuclear Cell Isolation Procedures for Gamma Interferon Enzyme-Linked Immunospot Testing of Healthy Swedish and Tanzanian Subjects
... Determination of antigen-specific T-cell responses is an important part of vaccine assessment. High levels of recovery, viability, and functionality of peripheral blood mononuclear cells (PBMCs) are essential for ...
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Diagnosis of Active Tuberculosis in China Using an In-House Gamma Interferon Enzyme-Linked Immunospot Assay
... ␥ enzyme-linked immunospot (ELISPOT) assay in the diagnosis of active tuberculosis (TB) in Shenzhen, China, where the prevalence of tuberculosis is severe and Mycobacterium bovis BCG vaccination is ...
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Enzyme-Linked Immunospot Assay for Detection of Human Respiratory Syncytial Virus F Protein-Specific Gamma Interferon-Producing T Cells
... Respiratory syncytial virus (RSV) causes significant disease in elderly adults, and we have previously reported that individuals 65 years of age and older have reduced RSV F protein-specific gamma interferon (IFN- ␥ ...
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Use of Enzyme Linked Immunospot Assay with Mycobacterium tuberculosis Specific Peptides for Diagnosis of Recent Infection with M tuberculosis after Accidental Laboratory Exposure
... From a venous blood sample peripheral blood mononuclear cells (PBMC) were isolated and an overnight gamma inter- feron (IFN- ␥ ) enzyme-linked immunospot (ELISPOT) assay was performed as previously ...
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Rotavirus-specific intestinal immune response in mice assessed by enzyme-linked immunospot assay and intestinal fragment culture.
... Oral inoculation of mice with WC3 induced low frequencies of virus-specific IgA-secreting cells by ELISPOT assay; this find- ing correlated with the small quantities of IgA secreted by G[r] ...
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Ganglioside GM1 enzyme linked immunospot assay for simple identification of heat labile enterotoxin producing Escherichia coli
... coli strains, results obtained by this procedure GM1ELISPOT correlated well with those of the GM1 enzyme-linked immunosorbent assay GM1-ELISA; in no instance were any false-positive reac[r] ...
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Enzyme-Linked Immunospot Assay Detection of Mumps-Specific Antibody-Secreting B Cells as an Alternative Method of Laboratory Diagnosis
... Although high measles, mumps, and rubella (MMR) vaccination coverage has been successful in dramat- ically reducing mumps disease in the United States, mumps (re)infections occasionally occur in individuals who have been ...
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Memory T-Cell Response to Rotavirus Detected with a Gamma Interferon Enzyme-Linked Immunospot Assay
... an enzyme-linked im- munospot (ELISPOT) assay with antigen-stimulated periph- eral blood mononuclear cells (PBMCs), from both adults and children recently infected with rotavirus, exhibited increased levels ...
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Evaluation of the Characteristics of the Enzyme-Linked Immunospot Assay for Diagnosis of Active Tuberculosis in China
... In patients with an acid-fast bacillus smear or in whom pathology with granuloma or caseous necrosis was detected, the T-SPOT.TB test had high positive rates (91.67% and 87.18%, respecti[r] ...
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Improved Assessment of T-Cell Receptor (TCR) VB Repertoire in Clinical Specimens: Combination of TCR-CDR3 Spectratyping with Flow Cytometry-Based TCR VB Frequency Analysis
... the enzyme-linked immunospot assay, ex vivo TCR variable (v)-segment analysis determined by flow cytometry, and TCR-CDR3 length analysis (spectratyping), as well as identifica- tion of ...
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Comparison of Two Gamma Interferon Release Assays and Tuberculin Skin Testing for Tuberculosis Screening in a Cohort of Patients with Rheumatic Diseases Starting Anti-Tumor Necrosis Factor Therapy
... by enzyme-linked immunosorbent assay (ELISA) (QuantiFERON-TB Gold [QFT-G] and QuantiFERON-TB Gold In Tube [QFT-GIT] assays; Cellestis Limited, Carnegie, Victoria, Australia) or by ...
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Analysis of Virus-Specific CD4+ T Cells during Long-Term Gammaherpesvirus Infection
... The IFN-␥ enzyme-linked immunospot ELISpot assay 5, 9 was used to screen peptide response profiles for CD4⫹ T cells enriched from the spleens of The murine gammaherpesvirus 68 ␥HV68 prov[r] ...
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Human Immunodeficiency Virus-Specific CD8+ T Cells in Human Breast Milk
... (IFN-␥) enzyme-linked immunospot (ELISPOT) assay was used to enumerate HIV-specific T cells from breast milk or peripheral blood mononuclear cells (PBMC) of HIV-infected or uninfected women ...
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A phase II trial of autologous dendritic cell vaccination and radiochemotherapy following fluorescence guided surgery in newly diagnosed glioblastoma patients
... Abbreviations 5-ALA: 5-aminolevulinic; CR: complete radiological resection; DC: dendritic cell; ELISA: enzyme-linked immunosorbent assay; ELISPOT: enzyme-linked immunospot assay; FGS: fl[r] ...
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