enzyme linked immunospot assay
Selected RD1 Peptides for Active Tuberculosis Diagnosis: Comparison of a Gamma Interferon Whole-Blood Enzyme-Linked Immunosorbent Assay and an Enzyme-Linked Immunospot Assay
... ) enzyme-linked immunospot assay (ELISPOT), using selected early secreted antigenic target 6 (ESAT-6) peptides, that appears specific for active tuberculosis ...whole-blood ...
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Enzyme-Linked Immunospot Assay Detection of Mumps-Specific Antibody-Secreting B Cells as an Alternative Method of Laboratory Diagnosis
... Although the sample size was small, we performed receiver operating characteristic (ROC) analysis to estimate the sensi- tivity and specificity of the assay and to determine the thresh- old of detection for each ...
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Evaluation of the Interlaboratory Concordance in Quantification of Human Immunodeficiency Virus-Specific T Cells with a Gamma Interferon Enzyme-Linked Immunospot Assay
... ELISPOT assay is highly sensitive and reproducible (48), and a multicenter comparative study of healthy donors with influenza virus and HIV peptides found that the four laboratories involved obtained consistent ...
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Varicella-Zoster Virus-Specific Enzyme-Linked Immunospot Assay Responses and Zoster-Associated Pain in Herpes Zoster Subjects
... Immunogenicity measurements. Blood was analyzed for VZV-CMI responses by IFN- ␥ ELISPOT assays performed on peripheral blood mononuclear cells (PBMCs) collected and frozen at the study sites. PBMCs were shipped on dry ...
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Diagnosis of Active Tuberculosis in China Using an In-House Gamma Interferon Enzyme-Linked Immunospot Assay
... ELISPOT assay. For development of an ELISPOT assay to detect specific IFN- ␥ responses against ...ELISPOT assay was 10 g/ml, as optimized by ...
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Rotavirus-specific intestinal immune response in mice assessed by enzyme-linked immunospot assay and intestinal fragment culture.
... Oral inoculation of mice with WC3 induced low frequencies of virus-specific IgA-secreting cells by ELISPOT assay; this find- ing correlated with the small quantities of IgA secreted by G[r] ...
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Ganglioside GM1 enzyme linked immunospot assay for simple identification of heat labile enterotoxin producing Escherichia coli
... coli strains, results obtained by this procedure GM1ELISPOT correlated well with those of the GM1 enzyme-linked immunosorbent assay GM1-ELISA; in no instance were any false-positive reac[r] ...
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Memory T-Cell Response to Rotavirus Detected with a Gamma Interferon Enzyme-Linked Immunospot Assay
... depletion assay which ensures that the antigen-presenting cells are present throughout the assay for the CD4 ⫹ IFN- ␥ T-cell response ...ELISPOT assay, Rojas et ...ELISPOT assay with IFN- ␥ ...
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Comparison of Enzyme-Linked Immunospot Assay and Tuberculin Skin Test in Healthy Children Exposed to Mycobacterium tuberculosis
... T-cell assay using M tuberculosis-specific antigens is not as sensitive for the diagnosis of M tuberculosis from recent exposure as the TST in the Gambia and offers no added benefit TABLE 2 Univariable and ...
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ESAT 6/CFP 10 Fusion Protein and Peptides for Optimal Diagnosis of Mycobacterium tuberculosis Infection by Ex Vivo Enzyme Linked Immunospot Assay in The Gambia
... Participants. TB index cases over 15 years of age were recruited from the major government health center in greater Banjul and the outpatient clinic at the Medical Research Council Laboratories in The Gambia. The cases ...
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Use of Enzyme Linked Immunospot Assay with Mycobacterium tuberculosis Specific Peptides for Diagnosis of Recent Infection with M tuberculosis after Accidental Laboratory Exposure
... From a venous blood sample peripheral blood mononuclear cells (PBMC) were isolated and an overnight gamma inter- feron (IFN- ␥ ) enzyme-linked immunospot (ELISPOT) assay was performed as ...
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Enzyme-Linked Immunospot Assay for Detection of Human Respiratory Syncytial Virus F Protein-Specific Gamma Interferon-Producing T Cells
... IFN-␥ enzyme-linked immunospot (ELISPOT) ...maximize assay sensitivity and precision. The optimized assay uses 300,000 cells/well, 2 g/ml of an RSV F peptide pool (RSV Fpp), and ...
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Detection of Human Papillomavirus Type 16-Specific T Lymphocytes by a Recombinant Vaccinia Virus-Based Enzyme-Linked Immunospot Assay
... The enzyme-linked immunospot (ELISPOT) assay is a sen- sitive and quantitative assay that has been widely used to detect antigen-specific cytokine-secreting T cells at the single-cell ...
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Evaluation of the Characteristics of the Enzyme-Linked Immunospot Assay for Diagnosis of Active Tuberculosis in China
... In patients with an acid-fast bacillus smear or in whom pathology with granuloma or caseous necrosis was detected, the T-SPOT.TB test had high positive rates (91.67% and 87.18%, respecti[r] ...
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Improved Assessment of T-Cell Receptor (TCR) VB Repertoire in Clinical Specimens: Combination of TCR-CDR3 Spectratyping with Flow Cytometry-Based TCR VB Frequency Analysis
... the enzyme-linked immunospot assay, ex vivo TCR variable (v)-segment analysis determined by flow cytometry, and TCR-CDR3 length analysis (spectratyping), as well as identifica- tion of ...
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Human Immunodeficiency Virus-Specific CD8+ T Cells in Human Breast Milk
... Breast-feeding infants of human immunodeficiency virus (HIV)-infected women ingest large amounts of HIV, but most escape infection. While the factors affecting transmission risk are poorly understood, HIV- specific ...
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Comparison of Two Gamma Interferon Release Assays and Tuberculin Skin Testing for Tuberculosis Screening in a Cohort of Patients with Rheumatic Diseases Starting Anti-Tumor Necrosis Factor Therapy
... by enzyme-linked immunosorbent assay (ELISA) (QuantiFERON-TB Gold [QFT-G] and QuantiFERON-TB Gold In Tube [QFT-GIT] assays; Cellestis Limited, Carnegie, Victoria, Australia) or by ...
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Potential Role of Immunodiagnosis for Pulmonary Tuberculosis Using Induced Sputum Cells
... Fig. 2. Concentration of interferon-gamma releasing cells reactive to early secretory antigenic target (ESAT)-6 and culture filtrate protein (CFP)-10 measured using enzyme-linked immunospot ...
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A phase II trial of autologous dendritic cell vaccination and radiochemotherapy following fluorescence guided surgery in newly diagnosed glioblastoma patients
... Abbreviations 5-ALA: 5-aminolevulinic; CR: complete radiological resection; DC: dendritic cell; ELISA: enzyme-linked immunosorbent assay; ELISPOT: enzyme-linked immunospot assay; FGS: fl[r] ...
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Asian Elephant T Cell Responses to Elephant Endotheliotropic Herpesvirus
... ) enzyme-linked immunospot assay for tracking antigen- directed T cell activity by monitoring rabies-specific responses in vaccinated ele- ...
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