ESAT-6
ESAT 6/CFP 10 Fusion Protein and Peptides for Optimal Diagnosis of Mycobacterium tuberculosis Infection by Ex Vivo Enzyme Linked Immunospot Assay in The Gambia
... The fusion protein of ESAT-6 (Rv3875) and CFP10 (Rv3874) was engineered as follows. The individual genes were amplified from M. tuberculosis H37Rv genomic DNA by PCR. During the amplification steps the ...
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Construction of Eukaryotic Expression Vectors Encoding CFP-10 and ESAT-6 Genes and Their Potential in Lymphocyte Proliferation
... Purified PCR products were subjected to two-step digestion with Xho I/Hind III and Not I/Xho I restriction enzymes for ESAT-6 and CFP-10, respectively. The purified, digested PCR products were ligated into ...
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The ESAT 6 gene cluster of Mycobacterium tuberculosis and other high G+C Gram positive bacteria
... five ESAT-6 cluster regions of ...the ESAT-6 gene clusters that do not have any homologs in the other clusters, suggesting subsequent insertions or deletions from the ancestral region ...
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Assessment of Cross-Reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-Cell Epitope Level
... like ESAT-6 and CFP-10 are used in the BOVIGAM gamma interferon (IFN- ␥ ) test, they have also been shown to enhance the specificity of the tuberculin-based test per se in this respect (7, 8, 20, 21, 26, ...
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Rapid Identification of the Mycobacterium tuberculosis Complex by Combining the ESAT 6/CFP 10 Immunochromatographic Assay and Smear Morphology
... teria. With positive BACTEC cultures that are also AFB pos- itive, colony isolation and identification are carried out using conventional biochemical methods for differentiation of MTC from nontuberculosis mycobacteria ...
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MODELING AND MOLECULAR DYNAMICS STUDIES ON ESP1 AND ESP6 EPITOPES OF EARLY SECRETED ANTIGENIC TARGET PROTEIN ESAT-6 FROM MYCOBACTERIUM TUBERCULOSIS
... In quest for finding new epitopes from ESAT-6 sequence, two novel epitopes were identified that are not reported earlier. Using Molecular dynamics, thermodynamically and conformationally stable ESP1 and ...
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ESAT 6–dependent cytosolic pattern recognition drives noncognate tuberculosis control in vivo
... protein 6-kDa early secretory antigenic target–mediated (ESAT-6–mediated) cytosolic contact, and activation of NLR family pyrin domain–containing protein 3 (NLRP3) inflammasomes in CD11c + cell ...
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Selected Pool of Peptides from ESAT 6 and CFP 10 Proteins for Detection of Mycobacterium tuberculosis Infection
... isolates with the exception of M. kansasii, M. marinum, M. szulgai, M. flavescens, and M. gastrii (6, 19, 31, 38). An ESAT-6 homologue has also been identified in the genome of M. leprae (18). Both ...
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Red Spectral Shift in Sensitive Colorimetric Detection of Tuberculosis by ESAT 6 Antigen Antibody Complex: a New Strategy with Gold Nanoparticle
... the ESAT-6 protein. ESAT-6 protein is the early secretary protein, identified as the important anti- gen in tuberculosis [23 – ...Diagnosing ESAT-6 pro- tein at an earlier stage, ...
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A T cell based assay using specific Mycobacterium tuberculosis antigens, ESAT-6 and CFP 10, for the diagnosis of Tuberculous Meningitis.
... target 6-kD protein (ESAT-6) was identified from culture filtrate because of its strong recognition in animals infected with M tuberculosis ...tuberculosis ESAT-6 gene was cloned and ...
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Influenza Virus NS Vectors Expressing the Mycobacterium tuberculosis ESAT-6 Protein Induce CD4+ Th1 Immune Response and Protect Animals against Tuberculosis Challenge
... the ESAT-6 protein in our constructs, presumably leading to an increased stability and immunogenicity of the foreign ...the ESAT-6 antigen after challenge in lymphocytes derived from the MLNs, ...
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Diagnosis of Tuberculosis Based on the Two Specific Antigens ESAT-6 and CFP10
... of ESAT-6 and CFP10 is highly specific for ...in ESAT-6-positive and -negative contacts, and this may give an indication of the po- tential of such antigens for the diagnosis of preclinical or ...
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Recognition of ESAT-6 Sequences by Antibodies in Sera of Tuberculous Nonhuman Primates
... Sera were collected serially from experimentally infected animals over the course of infection. The number of serial sera ranged from 5 to 17 per animal. Only one serum sample was available from naturally infected ...
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Serodiagnosis Efficacy and Immunogenicity of the Fusion Protein of Mycobacterium tuberculosis Composed of the 10-Kilodalton Culture Filtrate Protein, ESAT-6, and the Extracellular Domain Fragment of PPE68
... Indirect ELISA. Antibody levels were evaluated in the cell-free super- natants by a standard indirect enzyme-linked immunosorbent assay (ELISA) as previously described. Titers for the total immunoglobulin G (IgG) levels ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... Cloning of ESAT-6 andCFP-10. Each PCR product was ligated into pTZ57R ⁄T cloning vector after purification using QIA quick PCR purification kit (MBI Fermentas, Lithuania). Consequently, ligation mixture was ...
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Original Article Polyethylenimine modified nanoparticle adjuvant increases therapeutic efficacy of DNA vaccine Ag85A-ESAT-6-IL-21 in mice infected with Mycobacterium tuberculosis
... IL-21, and the coalescing small granulomas (the arrows) displayed extensively infiltrated numerous lymphocytes and neutrophils as is shown in Figure 1E. Whereas more lung tissues with extensive caseating and miliary ...
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ESAT-6-dependent cytosolic pattern recognition drives noncognate tuberculosis control in vivo
... The efficacy of subunit protein vaccines is dependent on the right choice of adjuvants (59), which activate appropriate PRRs such as TLRs (60, 61). In contrast, live attenuated vaccines rarely require adjuvants, as some ...
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Specific Delayed Type Hypersensitivity Responses to ESAT 6 Identify Tuberculosis Infected Cattle
... on ESAT-6, a recombinantly produced antigen highly specific for tuberculosis infection, were ...Although ESAT-6 was strongly recognized in vitro and induced high levels of gamma interferon, ...
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Comparative Analysis of Bacillus subtilis Spores and Monophosphoryl Lipid A as Adjuvants of Protein-Based Mycobacterium tuberculosis-Based Vaccines: Partial Requirement for Interleukin-17A for Induction of Protective Immunity
... well-characterized, 6-kDa early secretory antigenic target of M. tuberculosis (ESAT-6; Rv3875) was used as a test antigen to determine the T cell activation potential of each ...
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Highly Accurate Antibody Assays for Early and Rapid Detection of Tuberculosis in African and Asian Elephants
... the ESAT-6 and CFP10 proteins of ...of ESAT-6 has been reported for nonhuman primates infected with ...(5, 6, 12, 13, 14, 17, 19, ...not ESAT-6 or ...against ...
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