Escherichia coli BL21
Production of the short peptide surfactant DAMP4 from glucose or sucrose in high cell density cultures of Escherichia coli BL21(DE3)
... Results: In this work, the short peptide surfactant, DAMP4, was used as a model peptide to investigate production in Escherichia coli BL21(DE3), a classical strain used for protein production. Under ...
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A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3)
... Results: A two-step styrene biosynthesis pathway was developed and introduced into Escherichia coli BL21(DE3). Systematic optimization of styrene biosynthesis, such as enzyme screening, codon and ...
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The metabolic potential of Escherichia coli BL21 in defined and rich medium
... E. coli carbon metabolism. E. coli is a “Crabtree-positive” bacterium secreting acetate not only under conditions of oxygen limitation but also under “carbon excess” conditions presumably as a result of an ...
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Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of l-DOPA from d-glucose
... Microbial fermentation for l-DOPA production has pre- viously been focused on the catalyzation of l-tyrosine by tyrosinase [7] or through catechol and pyruvate from tyrosine phenol-lyase (TPL) [49–51]. The de novo pro- ...
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Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway
... E. coli BL21(DE3) with the empty pETDuet and pCDF plasmids was used as a control for plating to assess the burden imposed on the host by IPTG exposure in the absence of the heterologous pathway ...
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Activation of formate hydrogen-lyase via expression of uptake [NiFe]-hydrogenase in Escherichia coli BL21(DE3)
... and Escherichia coli (hydrogenase-1) in E. coli BL21(DE3), a strain that lacks H 2 -evolving ...wild-type BL21(DE3), the recombinant BL21(DE3) strains possessed formate ...
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Acetate: friend or foe? Efficient production of a sweet protein in Escherichia coli BL21 using acetate as a carbon source
... in BL21 in the pres- ence of acetate ...E. coli BL21 in typical autoinducing media, where addi- tional digestible carbohydrates are normally present ...
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Immunogenicity assay of KatG protein from Mycobacterium tuberculosis in mice: preliminary screening of TB vaccine
... в escherichia coli BL21 із використанням вектора ...e. coli BL21 одержували протеїн KatG із молекулярної масою 80 кДа за електрофорезу на ...
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High Yield Overexpression, Refolding, Purification and Characterization of Pseudomonas aeruginosa Type B-Flagellin: An Improved Method Without Sonication
... Pseudomonas aeruginosa as an opportunistic pathogen is a significant cause of acute and chronic infections in patients with compromised defenses. This bacterium is motile via a single polar flagellum made of polymerized ...
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Construction of a chimeric CotA protein bearing glutathione S transferase for improved enzyme activity
... Chimeric enzymes with novel catalytic combinations have been created for various purposes, especially to improve kinetic properties and catalytic performances of the parental enzymes. Herein, a bifunctional enzyme was ...
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False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid-Based Western Blot Assay Were Rectified by the Use of Two Subunits (S1 and S2) of Spike for Detection of Antibody to SARS-CoV
... Constructions of plasmids for expression of S1, S2, and N genes of SARS-CoV. (i) Spike protein. After computer analysis to predict and delete hydrophobic regions (hydrophobic cluster analysis), the spike protein of ...
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E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)
... Background: Escherichia coli is one of the most widely used hosts for recombinant protein production in academia and ...Strain BL21(DE3) is frequently employed due to its advantageous feature of ...
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Cloning of influenza B NS1 gene in Escherichia Coli
... Protein non-struktur NS1 virus influenza B adalah virulensi pelbagai fungsi dan terlibat dalam pengangkutan RNA virus. Di dalam sel perumah, NS1B menghalang sistem interferon α/β yang menyebabkan tindakbalas antivirus ...
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Phenotypic and Genotypic Characterization of EnteropathogenicEscherichia coli (EPEC) strains in Tehran, Iran
... E. coli (STEC) also harbor the eae gene (2, ...E. coli isolates carried the stx gene, thus we did not find any STEC strains, similar to results obtained from Taiwan and Tanzania ...
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MICROBIAL L-ASPARAGINASE: PRESENT AND FUTURE PROSPECTIVE
... from Escherichia coli remain stable at 37⁰C upto 4 h but after that the wild type enzyme rapidly loses its residual activity as compared to mutant ...
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Bacterial Secretions of Nonpathogenic Escherichia coli Elicit Inflammatory Pathways: a Closer Investigation of Interkingdom Signaling
... genes. BL21 and W3110 are, respectively, derivatives of the B and K-12 strains of ...E. coli, which comprise the majority of all laboratory ...of BL21 compared to blank media and W3110 compared to ...
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Molecular and Structural Characterization of a Novel Escherichia coli Interleukin Receptor Mimic Protein
... The agn43-yeeR-irmA genes are transcribed as a single tran- scriptional unit. To examine the transcriptional organization of the agn43-yeeR-irmA genes, we utilized a series of primers specific for each individual gene in ...
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EspZ of Enteropathogenic and Enterohemorrhagic Escherichia coli Regulates Type III Secretion System Protein Translocation
... richia coli, and enterohemorrhagic E. coli) to translocate effector proteins directly from the bacterial cytosol into eukaryotic cells (reviewed in reference ...
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Escherichia coli hemolysin is a potent inductor of phosphoinositide hydrolysis and related metabolic responses in human neutrophils
... Escherichia coli hemolysin (Hly) is a proteinaceous pore-forming exotoxin that probably represents a significant virulence factor in E. coli infections. We investigated its influence on human ...
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Production, Purification and Characterization of Chicken Egg Yolk Monoclonal Antibody Against Colonization factor antigen -1 of Enterotoxigenic Escherichia coli Causing Diarrhea
... PCR amplification and analysis of CFA-1 gene The 18 h old culture of E. coli (50 ml) was used for the isolation of plasmid DNA by different solutions (solution A: 50 mM glucose, 25 mM Tris- HCl, 10 mM EDTA; ...
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