Excretory/secretory antigen analysis
Analysis of Entamoeba histolytica Excretory-Secretory Antigen and Identification of a New Potential Diagnostic Marker
... Diagnosis of ALA is often initiated with radiology imaging to examine the presence of abscess in the liver. If indicated, aspiration of the sample is performed for culture, DNA detec- tion, and/or antigen ...
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Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections
... Sensitive and specific diagnostic tools are necessary in order to treat patients early and to avoid the major clinical compli- cations caused by the parasite. In this context, immunological probes are replacing direct ...
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Leishmanial Excretory-Secretory proteins: A potent vaccine candidate
... M. Analysis of immune responses directed toward a recombinant early secretory antigenic target six-kilodalton protein- culture filtrate protein 10 fusion protein in Mycobacterium bovis- infected ...vitro ...
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Immunoproteomic analysis of the excretory secretory products of Trichinella pseudospiralis adult worms and newborn larvae
... Conclusions: Early candidate antigens from the ES proteins of T. pseudospiralis have been screened and identified. Our results suggest these proteins may play key roles during the T. pseudospiralis infection and suppress ...
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Analysis of Diagnostic Efficiency of Excretory Secretory Antigens for Trichinella spiralis and Trichinella nativa in ELISA
... The analysis was performed by using indirect variant with polystyrene 96-pits strip microplates from Gosniipo- limer, ...sensitization excretory-secretory antigens with molecular mass of 29 - 63 kDa ...
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Genome wide analysis of excretory/secretory proteins in Echinococcus multilocularis: insights into functional characteristics of the tapeworm secretome
... Investigating the number of synonymous and non- synonymous substitutions along a lineage can provide information about the degree of selection for a species during evolution. Although the ES proteins were directly ...
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Immunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins
... Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the ...
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Comparative analysis of excretory-secretory antigens of Trichinella spiralis and Trichinella britovi muscle larvae by two-dimensional difference gel electrophoresis and immunoblotting
... p49 antigen variants (spots 24 and 60) in ...LC-MS/MS analysis of the tryptic peptides derived from these proteins could not be matched with recently released predicted proteins from ...LC-MS/MS ...
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Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC–ESI–MS/MS and LC–MALDI–TOF/TOF
... www.biomedcentral.com/submit Submit your next manuscript to BioMed Central and we will help you at every step: 37. Haque R, Mollah NU, Ali IKM, Alam K, Eubanks A, Lyerly D, Petri WA. Diag- nosis of amebic liver abscess ...
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Needles in the EST haystack: Large-scale identification and analysis of excretory-secretory (ES) proteins in parasitic nematodes using expressed sequence tags (ESTs)
... GnRH signaling pathway; linoleic acid metabolism; N-glycan biosynthesis; ATP synthesis; aminosugar metabolism; galactose metabolism; glycine, serine and threonine metabolism. Even though not well represented, their ...
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Analysis of excretory/secretory antigens obtained from helminth parasites
... immunosorbent assay of human sera for antibodies to excretory- secretory antigens of the second stage larvae of Toxocara canis. (1963) Effect of pH and calcium con[r] ...
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CiteSeerX — Proteomics Analysis of the Excretory/Secretory Component of the Blood-feeding Stage of the
... Advances in mass spectrometry and associated technolo- gies have now made possible the rapid and sensitive deter- mination of the protein content of biological fluids such as hookworm ES products. The traditional ...
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Development of a Highly Specific RecombinantToxocara canis Second Stage Larva Excretory Secretory Antigen for Immunodiagnosis of Human Toxocariasis
... canis antigen developed for the immunodiagnosis of human toxocariasis was compared with that of the excretory-secretory antigen from ...recombinant antigen was tested at a concentration ...
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Parasite excretory-secretory products and their effects on metabolic syndrome
... Accepted Article Helminths are not alone in their ability to modulate the host immune response to create an optimum environment – protozoan parasites, such as Leishmania and Toxoplasma, are able to impair the host’s ...
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Parasite excretory-secretory products and their effects on metabolic syndrome
... macrophages and DCs, and consequently can inhibit IFN- production 58 . Further promoting this TH1-TH2 switch, products from the parasite can also significantly enhance IL-4 production in the draining LN 59 . In ...
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Parasite excretory-secretory products and their effects on metabolic syndrome
... . Further promoting this TH1-TH2 switch, products from the parasite can also significantly enhance IL-4 production in the draining LN 59 . In addition, there is growing evidence to suggest that other intracellular ...
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Investigating the immunomodulatory properties of hookworm excretory/secretory (ES) products
... Gabriel SE, Gakidou E, Ganatra HA, Garcia B, Gaspari F, Gillum RF, Gmel G, Gonzalez- Medina D, Gosselin R, Grainger R, Grant B, Groeger J, Guillemin F, Gunnell D, Gupta R, Haagsma J, Hagan H, Halasa YA, Hall W, Haring D, ...
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Excretory/secretory products of anisakid nematodes: biological and pathological roles
... responses. Excretory and secretory (ES) com- pounds produced by the parasites are assumed to be key players in clinical manifestation of the disease in humans, but the molecules are likely to play a general ...
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Sm16, a major component of Schistosoma mansoni cercarial excretory/secretory products, prevents macrophage classical activation and delays antigen processing
... 0-3hRP is recognized by TLRs, whilst Sm16 is known to inhibit the function of these receptors [7,10]. Conse- quently, we investigated the distribution and function of Sm16 in different fractions of S. mansoni cercarial ...
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Sm16, a major component of Schistosoma mansoni cercarial excretory/secretory products, prevents macrophage classical activation and delays antigen processing
... 0-3hRP is recognized by TLRs, whilst Sm16 is known to inhibit the function of these receptors [7,10]. Conse- quently, we investigated the distribution and function of Sm16 in different fractions of S. mansoni cercarial ...
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