exonuclease domain
Rationale and design of the POLEM trial: avelumab plus fluoropyrimidine-based chemotherapy as adjuvant treatment for stage III mismatch repair deficient or POLE exonuclease domain mutant colon cancer: a phase III randomised study
... Background 10%–15% of early- stage colon cancers harbour either deficient mismatch repair (dMMR), microsatellite instability high (MSI- H) or POLE exonuclease domain mutations, and are characterised by high ...
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Low frequency of POLD1 and POLE exonuclease domain variants in patients with multiple colorectal polyps
... the exonuclease domain of POLE and ...the exonuclease domain of POLE and ...the exonuclease domain (EDM) was iden- tified in patient ...
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Accelerated Evolution of Maribavir Resistance in a Cytomegalovirus Exonuclease Domain II Mutant
... pol exonuclease domain II D413A mutation found in a clinical specimen, when transferred to a recombi- nant virus, resulted in an increased DNA replication error rate compatible with loss of ...
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Somatic POLE exonuclease domain mutations in endometrial cancer : Insights into the biology of POLE-mutant tumors
... Heatmap showing modelled probability that mutations in endometrial cancer driver genes (defined based on IntOGen – see Materials and methods, Definition of driver genes; Supplementary Table 5) were due to a prior POLE ...
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The Exonuclease Domain of Lassa Virus Nucleoprotein Is Involved in Antigen-Presenting-Cell-Mediated NK Cell Responses
... NK cells are activated by rNP-LASV-infected APC. As previously shown (19), we observed that in contrast to rWT-LASV, rNP- LASV was strongly attenuated in DC and M single cultures as well as NK/APC cocultures (data not ...
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Study protein-protein interaction in methyl-directed DNA mismatch repair in E. coli : exonuclease I (Exo I) and DNA helicas II (UvrD); A minimal exonuclease domain of WRN forms a hexamer on DNA and possesses both 3’-5’ exonuclease and 5’-protruding strand endonuclease activities; Solving the structure of the ligand-binding domain of the pregnane-xenobiotic-receptor with 17[beta] estradiol and T1317
... with 1 mM 3-amino-1,2,4-triazole (3-AT). 3-AT was required to suppress a low level of HIS3 activation by the pGBT9-UvrD DNA binding domain fusion. Transformation efficiencies were monitored by plating small ...
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Exonuclease Domain of the Lassa Virus Nucleoprotein Is Critical To Avoid RIG-I Signaling and To Inhibit the Innate Immune Response
... vation was completely abolished in APC infected with LASV-WT. Moreover, the stronger immunogenicity of LASV-ExoN than of MOPV suggests that the ExoN domain, which is also present in the MOPV NP, probably at least ...
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Locations of amino acid substitutions in bacteriophage T4 tsL56 DNA polymerase predict an N-terminal exonuclease domain.
... Identification of tsL56 mutations and characterization of tsL56 strains Mutation identification Strain Codon Amino acid' codon nucleotide sequence in: Wild type Wild type tsL56 430C Rel[r] ...
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Genetic characterization of the vaccinia virus DNA polymerase: cytosine arabinoside resistance requires a variable lesion conferring phosphonoacetate resistance in conjunction with an invariant mutation localized to the 3'-5' exonuclease domain.
... Mapping of the lesions conferring araCr by marker rescue of PAA' congenic isolates with subclones of five araCr DNA polymerase alleles DNA fragmente Titration of yield PFU/ml6 Recipient [r] ...
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The Drosophila Werner Exonuclease Participates in an Exonuclease-Independent Response to Replication Stress
... an exonuclease domain, the Drosophila melanogaster homolog, WRNexo, contains only the exonuclease ...the exonuclease functions of WRN separate from the ...an exonuclease-dead copy of ...
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Drosophila exoribonuclease nibbler is a tumor suppressor, acts within the RNAi machinery and is not enriched in the nuage during early oogenesis
... 3′-5′ exonuclease domain revealed that the sequence homology is fairly good ...an exonuclease domain. This domain is part of a large DEDD subfamily of exori- bonucleases [53], owing to ...
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Finger Domain Mutation Affects Enzyme Activity, DNA Replication Efficiency, and Fidelity of an Exonuclease-Deficient DNA Polymerase of Herpes Simplex Virus Type 1
... (replication), exonuclease proofread- ing (editing), and UL42 binding (5, 6, ...and exonuclease domains are con- served among the B family (or the ␣ -like) polymerases ...The exonuclease ...
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Regulation of DNA Polymerase Exonucleolytic Proofreading Activity: Studies of Bacteriophage T4 “Antimutator” DNA Polymerases
... C-terminal domain, the location et ...the exonuclease domain (see Nossal ...and exonuclease active cen- from the polymerase to the exonuclease active center, a ters are separated by a ...
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Germline mutations affecting the proofreading domains of POLE and POLD1 predispose to colorectal adenomas and carcinomas
... the exonuclease domain of class B DNA polymerases (Supplementary Figure 2a) and a Blast search against the Protein Data Bank ...the exonuclease domain (L479 in yeast DNA polymerase [PDB ID: ...
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Deep sequencing of mitochondrial DNA and characterization of a novel POLG mutation in a patient with arPEO
... The patient had slowly progressive bilateral ptosis and severely reduced horizontal and vertical gaze. Muscle biopsy showed slight variability in muscle fiber size, scattered ragged red fibers, and partial cytochrome c ...
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Amino Acid Residues Critical for the Interaction between Bacteriophage T7 DNA Polymerase and Escherichia coli Thioredoxin
... lymerization domain of T7 DNA polymerase, and the other three suppressor mutations (V3I, V32A, and A45T) reside within the 3 9 to 5 9 exonuclease ...and exonuclease domains, respec- ...
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DNA Polymerase Fidelity: From Genetics Toward a Biochemical Understanding
... This review summarizes mutagenesis studies, emphasizing the use of bacteriophage T4 mutator and antimutator strains. Early genetic studies on T4 identified mutator and antimutator variants of DNA polymerase that, in ...
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Functional insight into Maelstrom in the germline piRNA pathway: a unique domain homologous to the DnaQ-H 3'–5' exonuclease, its lineage-specific expansion/loss and evolutionarily active site switch
... SANTA domain [66]; an evolutionary selection against domain duplication together with the functional conservation, therefore, should account for the establish- ment of this ...MAEL domain expan- sion ...
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Functions of Multiple Exonucleases Are Essential for Cell Viability, DNA Repair and Homologous Recombination in recD Mutants of Escherichia coli
... 59–39 exonuclease activity of RecJ, ExoVII is required for recombination and repair, whereas in mutants devoid of activities of 39–59 ssExos (ExoI and ExoX), ExoVII interferes with ...59–39 exonuclease ...
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Immobilization-Free Strategies for Designing of DNA-Based Electrochemical Biosensors
... T7 exonuclease-aided target recycling amplification was proposed for the detection of human telomerase activity (Figure 8) ...T7 exonuclease-catalyzed digestion of MB-labeled HP probe was initiated, leading ...
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