Exotoxin A
Characterization of monoclonal antibody B7, which neutralizes the cytotoxicity of Pseudomonas aeruginosa exotoxin A.
... Pseudomonas aeruginosa is an opportunistic pathogen which has become a major cause of nosocomial infections in immu- nocompromised patients, such as individuals with cystic fibro- sis, severe burns, or neoplasias or ...
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Monoclonal antibody C242 Pseudomonas exotoxin A A specific and potent immunotoxin with antitumor activity on a human colon cancer xenograft in nude mice
... Two immunotoxins were constructed by chemically coupling the monoclonal antibody C242 to Pseudomonas exotoxin A (PE) or a modified form, NlysPE40, that lacks the cell binding domain of PE. Monoclonal antibody C242 ...
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Hybrid proteins between Pseudomonas aeruginosa exotoxin A and poliovirus 2Apro cleave p220 in HeLa cells.
... Hybrid proteins consisting of toxins and proteins that selec- tively bind to target cells are becoming promising tools to specifically kill tumor and human immunodeficiency virus-in- fected cells. The domain of the toxin ...
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A mutant CHO-K1 strain with resistance to Pseudomonas exotoxin A and alphaviruses fails to cleave Sindbis virus glycoprotein PE2.
... DISCUSSION In this article we report that RPE.40, a mutant strain of CHO-Kl cells selected for resistance to Pseudomonas exotoxin A and cross-resistant to alphaviruses 21, fails to cleav[r] ...
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Production of elastase, exotoxin A, and alkaline protease in sputa during pulmonary exacerbation of cystic fibrosis in patients chronically infected by Pseudomonas aeruginosa
... cific for elastase and/or alkaline protease (83 to 88%) (9) indicates that in vivo production of these enzymes is common in patients with CF. These enzymes may therefore contribute to the pathogenesis of the disease. The ...
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Anthrax Toxin Mediated Delivery of the Pseudomonas Exotoxin A Enzymatic Domain to the Cytosol of Tumor Cells via Cleavable Ubiquitin Fusions
... vation of the receptor-bound protective antigen (PA) protein by cell surface proteases (5–7). Replacing the site normally cleaved by furin and related proteases with sequences recognized by matrix metalloproteases or ...
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Comparative analysis of serum antibody responses to Pseudomonas aeruginosa exotoxin A by cystic fibrosis and intensive care unit patients
... Comparative Analysis of Serum Antibody Responses to Pseudomonas aeruginosa Exotoxin A by Cystic Fibrosis and Intensive Care Unit Patients.. Pulmonary infection with Pseudomonas aeruginos[r] ...
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Protective activity of antibodies to exotoxin A and lipopolysaccharide at the onset of Pseudomonas aeruginosa septicemia in man
... Serum antibodies to exotoxin A and type-specific lipopolysaccharide were measured by passive hemagglutination in 52 patients with Pseudomonas aeruginosa septicemia. Their comparative protective activities were ...
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Biotinylated DNA probes for exotoxin A and pilin genes in the differentiation of Pseudomonas aeruginosa strains
... The variability in the coding region results in the presence or absence of unique restriction sites yielding unique hybridization patterns following Southern blot analysis of digested DN[r] ...
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Retrograde transport pathways utilised by viruses and protein toxins
... Cholera toxin [9,10], Shiga and the very closely related Shiga-like toxins (STx family) [11], Pseudomonas exotoxin A (PEx) [12] and the plant toxin ricin [13] seem unable to disrupt cellular membranes directly. ...
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CD4 Pseudomonas exotoxin conjugates delay but do not fully inhibit human immunodeficiency virus replication in lymphocytes in vitro
... CD4-Pseudomonas exotoxin A (PE) conjugates inhibit the proliferation of gp160-transfected Chinese hamster ovary cells and block HIV replication in virus-infected H9 ...
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The Group A Streptococci Bacteriocins Facilitate a Competitive Advantage During Nasopharyngeal Infection
... (84), we show that expression of human MHC class II molecules HLA-DR4/DQ8 and the streptococcal pyrogenic exotoxin A (SpeA) results in a robust infection phenotype observed in transgen[r] ...
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Production of leukocidin by clinical isolates of Pseudomonas aeruginosa and antileukocidin antibody from sera of patients with diffuse panbronchiolitis
... The present investigation proved that almost all clinical isolates produce leukocidin as well as exotoxin A, protease, and elastase and that the mean antileukocidin titer in the sera of [r] ...
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Molecular Detection of Some Virulence Traits Among Pseudomonas aeruginosa Isolates, Hilla-Iraq
... includes Exotoxin A (ETA), outer membrane associated protein L and I and quorum-sensing determinant ...system[14]. Exotoxin A represent the main dangerous virulence factor produced by ...
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Molecular detection of virulence factors and biofilm formation in Pseudomonas aeruginosa obtained from different clinical specimens in Bandar Abbas
... cemia, pneumonia, endocarditis, burn wounds, oti- tis and keratitis (5, 6). P. aeruginosa is responsible for mortality rates as high as 50%. It may quickly become problematic once introduced in a hospital because of its ...
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“Production, Isolation and Characterization of Exotoxin Produced by Bacillus subtilis and Bacillus megaterium and Proteus vulgaris and Its Significance in Food Poisoning” by Jai Ghosh, Sagar Barale, India.
... of exotoxin was checked by SDS-PAGE, by using ...of exotoxin of Bacillus subtilis, Bacillus megaterium and Proteus vulgaris was determined on a calibrated scale with standard marker enzyme (Phosphorylase b ...
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Molecular Characterization of Exotoxin Genes in Staphylococcus aureus Recovered From Hospitalized Patients
... To the best of our knowledge, the current study is one of the few researches investigating the frequency of exotoxin genes of MRSA isolated from patients in Iran. In the present study, the results showed that the ...
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Pseudomonas exotoxin anti TAC Cell specific immunotoxin active against cells expressing the human T cell growth factor receptor
... A toxic protein conjugate, designated PE-anti-TAC, was made by chemically coupling pseudomonas exotoxin PE to a monoclonal antibody anti-TAC that recognizes the human TCGF receptor.. Thi[r] ...
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A novel bispecific immunotoxin delivered by human bone marrow-derived mesenchymal stem cells to target blood vessels and vasculogenic mimicry of malignant gliomas
... and synthesized a BIT, VEGF 165 -ephrin A1-PE38KDEL, consisting of truncated Pseudomonas exotoxin (PE) and two different ligands, specifically, in which VEGF 165 targeted the VEGFR and ephrin A1 targeted the EphA2 ...
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Molecular characterization and evaluation of the emerging antibiotic resistant Streptococcus pyogenes from eastern India
... Keywords: Streptococcus pyogenes GAS, emm typing, vir typing, Virulence regulon, Restriction enzyme digestion, Antibiotic sensitivity patterns, Exotoxin gene.. Background Streptococcus p[r] ...
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