Expression cassette
Generation of mini-Tn7 transposone by cloning of CPV-VP2 into eukaryotic expression cassette, in E. coli
... Baculovirus Expression System facilitates rapid and efficient generation of recombinant ...Baculovirus Expression System takes advantage of the site-specific transposition properties of the Tn7 transposon ...
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Dual gene expression cassette vectors with antibiotic selection markers for engineering in Saccharomyces cerevisiae
... poor expression in ...of expression, while β-galactosidase activity driven by the GAL10 promoter was much ...gene expression plasmids (Table 1, Figure ...
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Inducible Overexpression of a Toxic Protein by an Adenovirus Vector with a Tetracycline-Regulatable Expression Cassette
... ideal expression cassette should be regulated in such a way that the basal level of transgene expression is low enough to avoid any interference with adenovirus replication and yet is capable of ...
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Promoter engineering enables overproduction of foreign proteins from a single copy expression cassette in Bacillus subtilis
... Plasmid-mediated recombinant production of pro- teins in bacteria is unstable during the late stage of fer- mentation [8]. Moreover, the safety concerns and legal requirements surrounding the use of antibiotic is another ...
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Enhancing functional production of a chaperone-dependent lipase in Escherichia coli using the dual expression cassette plasmid
... functional expression of the lipases in ...date, expression of in vivo functional lipase in heterologous host ...dual expression cassette plasmid system has not been ...
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Bioluminescence Imaging of Live Infected Salmonids Reveals that the Fin Bases Are the Major Portal of Entry for Novirhabdovirus
... We previously described a recombinant rIHNV- ⌬ NV in which the NV gene encoding a nonstructural protein of un- known role was deleted and replaced by the reporter green fluorescent protein (GFP) gene (4). This virus is ...
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The Major Portal of Entry of Koi Herpesvirus in Cyprinus carpio Is the Skin
... LUC expression cassette using the two-step procedure de- picted in ...LUC expression cassette, re- sulting in the FL BAC 136 LUC plasmid ...galK cassette (which does not contain a SacI ...
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Delta 6-desaturases From Primulaceae, Expressing Plants And Pufa-containing Oils (Patent EP 1726652 A2)
... The expression cassette may also contain a chemically inducible promoter by means of which the expression of the exogenous ∆ 6- desaturase gene in the organisms can be controlled advantageously in ...
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Novel sul I binary vectors enable an inexpensive foliar selection method in Arabidopsis
... terminator cassette was excised from the pUC backbone by HindIII and EcoRI digestion and cloned into pCAMBIA 390A (described ...pK-SulI cassette (described above) was cloned into the HindIII site in an ...
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Delta 6-desaturases From Primulaceae, Expressing Plants And Pufa-containing Oils (Patent EP 1481063 B1)
... The expression cassette may also contain a chemically inducible promoter by means of which the expression of the exogenous ∆ 6- desaturase gene in the organisms can be controlled advantageously in ...
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Conditional repression of the E2 transcription unit in E1-E3-deleted adenovirus vectors is correlated with a strong reduction in viral DNA replication and late gene expression in vitro.
... lacZ expression cassette was inserted into the unique BamHI site of pTG9549 reconstituting an adenovirus vector genome (DE1 lacZ DE3 tetO ...lacZ expression cassette was replaced by a CMV ...
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Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
... Results: In this study, we developed a CRISPR–Cas9-assisted multiplex genome editing (CMGE) approach includ- ing multiplex genes knock-outs, multi-locus (ML) and multi-copy (MC) integration methods in yeasts. Based on ...
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A Versatile Transreplication-Based System To Identify Cellular Proteins Involved in Geminivirus Replication
... the expression cassette may have arisen from recombination rather than replication, it was mandatory to identify the replicative intermediates in closer ...
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Multiple shRNA combinations for near-complete coverage of all HIV-1 strains
... shRNA expression plasmids used here as PCR templates were selected and constructed as previously described [12,50] (Additional file ...shRNA expression cassettes were common to all plasmids; the forward ...
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Construction of a novel fungal gus expression plasmid, and its evaluation in Aspergillus nidulans : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University
... Vectors used for transformation in filamentous fungi contain, i the expression cassette containing the DNA sequence to be expressed attached to fungal regulatory elements, ii a bacterial[r] ...
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Delta 6-desaturases From Primulaceae, Expressing Plants And Pufa-containing Oils (Patent EP 1726652 B1)
... the expression cassette (= nucleic acid construct or fragment or gene construct) it is meant the sequences specified in SEQ ID NO: 1 and SEQ ID NO:3 which result from the genetic code and/or functional or ...
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Inducible Expression of Human Gamma Interferon
... controlled expression of foreign proteins greatly aids selection of highly producer ...cumate-regulatable expression cassette that its background backs to the pseudomonas cumate operon ...
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Geminivirus C3 Protein: Replication Enhancement and Protein Interactions
... mutated expression cassette to that in the presence of the wild-type C3 expression cassettes and plotted against the central position of the mutated ...The expression cassettes in the first ...
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Tetracycline-Inducible Gene Expression and Gene Deletion in Candida albicans
... this cassette in different ...1). Expression of the multidrug efflux pumps did not affect the susceptibility of the strains to nourseothricin, indicating that this drug is not a substrate of these ...
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Synergistic antitumoral efficacy of a novel replicative adenovirus SG611-PDCD5 and daunorubicin in human leukemic cells
... gene expression cassette SG611-PDCD5, in which the E1a gene with 24 nucleotides deleted from the CR2 region is controlled by the hTERT promoter, the E1b gene is controlled by the HRE promoter, and PDCD5 is ...
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