firefly luciferase
Monitoring immediate-early gene expression through firefly luciferase imaging of HRS/J hairless mice
... the firefly luciferase gene (luc) are useful for examining gene regulation in live transgenic mice and they provide unique views of functioning ...expressing luciferase can be observed by imaging ...
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In silico study to breaking mystery of bioluminescence protein structure of bacterial luciferase and firefly luciferase
... Luciferase protein structure from firefly has been extensively studied from firefly Photinus pyralis ...[11]. Firefly luciferase was extensively used in molecular biology as a reporter ...
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Development of a Firefly Luciferase Based Assay for Determining Antimicrobial Susceptibility of Mycobacterium aviumsubsp paratuberculosis
... the luciferase gene from Photinus pyralis, the American firefly; these methods have been described elsewhere (1, 14, 15, 19, ...a firefly luciferase-based method for determination of the drug ...
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Firefly luciferase ATP assay as a screening method for bacteriuria
... Percentage of positive luciferase tests in routine culture specimens with yields of >105 CFU/ml when the ATP limit defining a positive test was varied.. D Gram-positive species: Staphylo[r] ...
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Longitudinal Bioluminescence Imaging of the Dynamics of Doxorubicin Induced Apoptosis
... cyclic firefly luciferase (pcFluc-DEVD) by fusing two fragments of DnaE in- tein to neighboring ends of luciferase separated by ...cyclic luciferase occurs, resulting in the restoration of ...
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Isolation and Characterization of Broad-Spectrum Disease-Resistant Arabidopsis Mutants
... signal transduction. Plant Physiol. 114: 751–757. hold et al., 1991 Coordinate gene activity in response to agents Ow, D., K. V. Wood, M. DeLuca, J. R. DeWet, D. R. Helinski et al., that induce systemic acquired ...
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Abortively Infected Astrocytes Appear To Represent the Main Source of Interferon Beta in the Virus-Infected Brain
... express firefly luciferase under the con- trol of the IFN- promoter in either all or only distinct cell types, we found that astrocytes are the main producers of IFN-  after infection of the brain with ...
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Synthesis and in vitro bioevaluation of 1,5 Bis(5 substituted benzimidazole) alkanes as antileishmanial agents
... with firefly luciferase gene, and the transfectants were maintained in medium 199 supplemented with 10% foetal calf serum, 1% penicillin (50 µ g/ml), and streptomycin (50 µ g/ml) solution (sigma) under ...
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Disruption of Type I Interferon Signaling by the Nonstructural Protein of Severe Fever with Thrombocytopenia Syndrome Virus via the Hijacking of STAT2 and STAT1 into Inclusion Bodies
... SFTSV infection suppresses type I IFN signaling. Type I IFN signaling results in the activation of the ISRE promoter and mounts the transcription of ISGs. To investigate whether SFTSV can interfere with the IFN ...
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The Interferon Antagonist ML Protein of Thogoto Virus Targets General Transcription Factor IIB
... encodes firefly luciferase under the control of an artifi- cial promoter containing three IRF3-binding sites ...and luciferase activity induced by IRF3 was measured 24 h later ...
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Characterization of the Unusual Bidirectional ves Promoters Driving VESA1 Expression and Associated with Antigenic Variation in Babesia bovis
... of firefly luciferase in a dual- luciferase assay, each demonstrated significant promoter activity relative to the promoterless control, pLuc-T3 ...with luciferase constructs containing the ...
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Mutational Analysis of the Varicella-Zoster Virus ORF62/63 Intergenic Region
... and firefly luciferase genes, which acted as reporters of ORF62 and ORF63 transcription, and recombinant viruses were generated that carried these reporter cassettes along with the intact native sequences ...
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Establishment of transient and stable transfection systems for Babesia ovata
... the firefly luciferase gene was amplified by PCR from the plasmid pEN- T12luc using specific primers (Additional file 1: Table S1) and inserted into the EcoRV site of pBluescript plas- mid vector using an ...
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A TNF Variant that Associates with Susceptibility to Musculoskeletal Disease Modulates Thyroid Hormone Receptor Binding to Control Promoter Activation
... the firefly-luciferase reporter under the control of the ‘A’ (panels A-C) or the ‘G’ variant (panel D) of the TNF promoter, and co-transfected with 30ng of TR-α expression plasmid (panel B) or with non- ...
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The human somatostatin receptor type 2 as an imaging and suicide reporter gene for pluripotent stem cell-derived therapy of myocardial infarction
... Fluc: firefly luciferase; GCV: ganciclovir; GVHD: graft-versus-host disease; hNIS: human sodium iodide symporter; hSSTr2: human somatostatin receptor type 2; HSV-tk: herpes simplex virus type 1 thymidine ...
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Effects of receptor tyrosine kinase inhibitors on VEGF165a and VEGF165b stimulated gene transcription in HEK 293 cells expressing human VEGFR2
... the firefly luciferase gene luc2P containing a human sequence enriched in proline (P), glutamic acid (E), serine (S) and threonine (T) protein desta- bilization sequence (Voon et ...
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Tombusvirus P19 RNA silencing suppressor (RSS) activity in mammalian cells correlates with charged amino acids that contribute to direct RNA-binding
... a Firefly luciferase (Fluc), a Renilla luciferase (Rluc), and a shRNA (small hairpin RNA) targeting Firefly luciferase mRNA (sh-Fluc) ...
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Towards the Development of a MagA Reporter Gene Expression Construct for Magnetic Resonance Imaging
... and firefly luciferase (Figure ...and luciferase, for multi- modality detection using MRI and ...activity. Luciferase activity may be quantified and used as a comparison to changes in cellular ...
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Mucosal gene therapy using a pseudotyped lentivirus vector encoding murine interleukin-10 (mIL-10) suppresses the development and relapse of experimental murine colitis
... expressing firefly luciferase (fLuc) on either colon with (a) PBS only, (b) with PBS followed by 3% DSS, (c) with 20% EtOH only, or (d) with 20% EtOH followed by 3% ...
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Synergistic Action of GA-Binding Protein and Glucocorticoid Receptor in Transcription from the Mouse Mammary Tumor Virus Promoter
... Plasmid construction. The wild-type MMTV LTR (GR strain; from the PstI site at position 11 in the LTR to the PvuII site at its 3⬘ end: pLSwt [36]) and the mutants LS ⫺175/⫺166 and ⌬⫺193/⫺162 (carrying an octameric ...
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