Fixed human stem cell derived cardiomyocytes
Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes
... single cell studies as well as most two-dimensional and three- dimensional culture systems usually require enzymatic ...substrate, cell density and timing of experimentation are critical ...whole ...
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Automated electrophysiological and pharmacological evaluation of human pluripotent stem cell derived cardiomyocytes
... In this study, we overcame these problems by developing a two-step cell dissociation protocol. First, intact EB/ monolayer cultures of hPSC-CMs were dissociated into single cells using a trypsin-based dissociation ...
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Mathematical modelling of the action potential of human embryonic stem cell derived cardiomyocytes
... We also tested coupling of fibroblasts with the hESC-CM model. Our hypothesis was that such approach could improve the quality of simulated APs that are generated by a complex system comprising CMs embedded in a cluster ...
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Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes
... While conventional methods such as the manual patch clamp provide detailed information of the electrical activity of cardiomyocytes, the complexity of such tech- nique necessitates the professional training of the ...
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Keywords: human pluripotent stem cells; cardiomyogenesis; stem cell-derived cardiomyocytes; differentiation; small molecules.
... various stem cell lines which can be used to produce stem-cell-derived cardiomyocytes for applications like drug discovery and regenerative medicine or to probe the signaling ...
Deep phenotyping of human induced pluripotent stem cell–derived atrial and ventricular cardiomyocytes
... Discussion In this study, we applied simplified and efficient protocols for differentiation of human iPSCs into defined populations of atrial or ventricular CM subtypes based on monolayer and feeder-free culture ...
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GLUT4 expression and glucose transport in human induced pluripotent stem cell-derived cardiomyocytes
... pluripotent stem cell derived cardiomyocytes (iPSC-CM) have the potential to trans- form regenerative cardiac medicine and the modelling of cardiac ...of cardiomyocytes was ...
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Perspectives on the Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Biomedical Research
... the human heart. The advent of pluripotent stem cell technology that allows cardio- vascular cells to be derived from patients and healthy individ- uals, in some cases genetically matched ...
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Can human pluripotent stem cell derived cardiomyocytes advance understanding of muscular dystrophies?
... As explained above, the breath of approaches to phenotype cell is expanding with a greater reliance on high content platforms, which will increase the throughput available. However, to confirm the pheno- type and ...
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Metabolomics Identifies Metabolic Markers of Maturation in Human Pluripotent Stem Cell-Derived Cardiomyocytes
... worldwide. Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) hold immense clinical potential and recent studies have enabled generation of virtually pure hPSC-CMs with high ...
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Can human pluripotent stem cell-derived cardiomyocytes advance understanding of muscular dystrophies?
... As explained above, the breath of approaches to phenotype cell is expanding with a greater reliance on high content platforms, which will increase the throughput available. However, to confirm the pheno- type and ...
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Predicting Induced Pluripotent Stem Cell Derived Cardiomyocytes Differentiation Outcomes
... pluripotent stem cell derived cardiomyocytes (iPSC-CMs) have revolutionized cardiovascular ...pluripotent stem cells (iPSCs), cells capable of becoming any cell type in the body, ...
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Embryonic stem cell-derived cardiomyocytes for the treatment of doxorubicin-induced cardiomyopathy
... transplanted cardiomyocytes in the myocardium suggests secretion of paracrine medi- ators by the transplanted cells, rather than cellular ...of cell death and five proteins connected to the regulation of ...
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Global transcriptome analysis of murine embryonic stem cell derived cardiomyocytes
... - cardiomyocytes derived from ES cells strongly express genes required for regulation of respiratory energy metabolism, cytoskeleton, and ion channels; these genes are required for cardiomyocytes to ...
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Simultaneous Measurement of Contraction and Calcium Transients in Stem Cell Derived Cardiomyocytes
... have been done without using corrections with a flu- orescent dye. Our results indicate that MQD based analysis of CM motion is minimally affected by the presence of a fluorescence based measurement. In t-tests, there were ...
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Induced pluripotent stem cell–derived cardiomyocytes in studies of inherited arrhythmias
... The last characterization of iPS-derived myocytes from LQTS patients was performed by Yazawa et al. in two individuals affected by Timothy syndrome (LQT8) (36). LQT8 is caused by mutations in the gene encoding the ...
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Cardiac repair in a murine model of myocardial infarction with human induced pluripotent stem cell-derived cardiomyocytes
... and cell therapy, there are several major hurdles that need to be addressed prior to their clinical ...functional cardiomyocytes prior to clinical application; such methods could in- volve single small ...
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Tissue-engineered cardiac patch seeded with human induced pluripotent stem cell derived cardiomyocytes promoted the regeneration of host cardiomyocytes in a rat model
... of stem cell or progenitor cell ...that cell transplantation upregulates various cardioprotective factors in native cardiac tissue through “crosstalk” between transplanted cells [12, ...host ...
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Uninterrupted monitoring of drug effects in human induced pluripotent stem cell derived cardiomyocytes with bioluminescence Ca2+ microscopy
... Results: GmNL(Ca 2 + ) gives a 140% signal change with Ca 2 + , and can image drug-induced changes of Ca 2 + dynam- ics in cultured cells. Since bioluminescence requires application of a chemical substrate, which is ...
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Developmental cues for the maturation of metabolic, electrophysiological and calcium handling properties of human pluripotent stem cell-derived cardiomyocytes
... Our laboratory has recently developed a highly cost- effective and efficient system for deriving hPSC-CMs from hESC (HES2, H7, H9) and iPSC lines [13]. This protocol, based on EB formation, requires minimal reagents (no ...
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