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flow cytometric analysis

Microfluidics for flow cytometric analysis of cells and particles

Microfluidics for flow cytometric analysis of cells and particles

... for flow cytometric analysis of cells and particles R95 liquids, etc), coupled with unconventional driving forces for fluid flows ...simple flow geometries, gas–liquid two-phase flows can ...

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Four-Color Flow Cytometric Analysis of Myeloma Plasma Cells

Four-Color Flow Cytometric Analysis of Myeloma Plasma Cells

... a flow cytometric technique using 4- color staining against CD38/CD45/CD19/CD56, immunofix- ation, and PCR ...approaches. Flow cytometric analysis has not only been shown to be ...

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Immunofluorescent Staining of Cell Surface Molecules for Flow Cytometric Analysis of Immune Function

Immunofluorescent Staining of Cell Surface Molecules for Flow Cytometric Analysis of Immune Function

... multicolor flow cytometric analysis for cytokine receptors and other cell surface antigens, it is recommended that PE- and APC-labeled antibodies be used for staining antigens that are expressed at ...

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Flow cytometric analysis of peripheral blood

Flow cytometric analysis of peripheral blood

... The proportion of Leu-7+, CD8+ lymphocytes and Leu-7+, CD3+ cells was significantly higher in inactive disease groups than in control subjects (inactive ulcerative colitis: p<OOOl, in[r] ...

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Automated Flow Cytometric Analysis of Cerebrospinal Fluid

Automated Flow Cytometric Analysis of Cerebrospinal Fluid

... Rapid flow cytometric counting of bacteria might be of interest in CSF analysis, where the ability to make a prompt tentative diagnosis of meningitis has important implications for the management and ...

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Flow Cytometric Analysis of Microsporidia Belonging to the Genus Encephalitozoon

Flow Cytometric Analysis of Microsporidia Belonging to the Genus Encephalitozoon

... By flow cytometry, formalin-treated and nontreated spores of Encephalitozoon were identified to the species level by using gated data on light-scatter profiles and analyzing the fluorescence histograms from the ...

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Flow Cytometric Analysis of Cytologic Specimens in Hematologic Disease

Flow Cytometric Analysis of Cytologic Specimens in Hematologic Disease

... The other 2 cases in which cytologic examination was suspicious for lymphoma were found to be benign by immunophenotyping; one, a pleural fluid from a case of follicular small cl[r] ...

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Flow cytometric Analysis of Interrelated Cancer - Interrelated Infection

Flow cytometric Analysis of Interrelated Cancer - Interrelated Infection

... and analysis were performed on a Becton Dickinson LSRII flow cytometer using 488- and 633-nm ...the analysis of major T cell–associated antigens and CD26, an arbitrary cursor was established based on ...

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DNA flow cytometric analysis in variable types of hydropic placentas

DNA flow cytometric analysis in variable types of hydropic placentas

... Histograms were generated from analysis of 10000 nuclei and displayed as linear fluorescence. As the use of internal standard controls, the first peak in the histograms was considered to represent diploid cells. ...

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Viability of cell cultures was determined by flow cytometric analysis using Fixable

Viability of cell cultures was determined by flow cytometric analysis using Fixable

... scatterplots of eFluor viability dye staining as analyzed by flow cytometry after different treatments. B: quantification of viable cells based on eFluor viability staining. Neither treatment with IL- 1β nor ...

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FLOW CYTOMETRIC ANALYSIS OF IMMUNOADJUVANT ACTIVITY OF EMBLICA OFFICINALIS ON HUMAN WHOLE BLOOD

FLOW CYTOMETRIC ANALYSIS OF IMMUNOADJUVANT ACTIVITY OF EMBLICA OFFICINALIS ON HUMAN WHOLE BLOOD

... centrifugation at 1800 rpm for 10 minutes, the supernatant was removed or aspirated and washed two- three times with phosphate buffered saline. After centrifugation, pellet dissolved in PBS and observed the cells through ...

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THE POTENTIAL CONTRIBUTION OF FLOW CYTOMETRIC ANALYSIS IN DIAGNOSIS AND SCORING OF MYELODYSPLASTIC SYNDROME PATIENTS

THE POTENTIAL CONTRIBUTION OF FLOW CYTOMETRIC ANALYSIS IN DIAGNOSIS AND SCORING OF MYELODYSPLASTIC SYNDROME PATIENTS

... in flow cytometry scores with increasing cytogenetic risk by IPSS criteria ...patients, flow scores and IPSS cytogenetic categories were disparate where 42 % of patients with normal cytogenetics (good risk) ...

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Flow cytometric analysis of platelets type 2 diabetes mellitus reveals ‘angry’ platelets

Flow cytometric analysis of platelets type 2 diabetes mellitus reveals ‘angry’ platelets

... Statistical analysis For each participant the MCFI was calculated as the mean fluorescence of a large sample of platelets (10,000 platelets per individual), the well-known Central Limit Theorem assures us that the ...

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Flow Cytometric Analysis of Leishmania Reactive CD4 + /CD8 + Lymphocyte Proliferation in Cutaneous Leishmaniasis

Flow Cytometric Analysis of Leishmania Reactive CD4 + /CD8 + Lymphocyte Proliferation in Cutaneous Leishmaniasis

... The functions include class-specific MHC-re- stricted recognition of antigens, capacity to pro- liferate and produce a range of cytokines. Dif- ferent features of proliferation might be seen in response to the same ...

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Clinical Course and Flow Cytometric Analysis of Paroxysmal Nocturnal Hemoglobinuria in the United States and Japan

Clinical Course and Flow Cytometric Analysis of Paroxysmal Nocturnal Hemoglobinuria in the United States and Japan

... our analysis, initial symptoms were quite different in the 2 cohorts of ...with flow cytometric data were registered from aplastic anemia ...with flow cytometric data) and as a smaller ...

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Ultra-Fast and Optimized Method for the Preparation of Rodent Testicular Cells for Flow Cytometric Analysis

Ultra-Fast and Optimized Method for the Preparation of Rodent Testicular Cells for Flow Cytometric Analysis

... Summarizing, we have developed a very simple, fast, and reproducible cell suspension-preparation method for flow cytom- etry analysis of rodent testicular cell populations. Although not tested here, this ...

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Multiparameter flow cytometric analysis of CD4 and CD8 T cell subsets in young and old people

Multiparameter flow cytometric analysis of CD4 and CD8 T cell subsets in young and old people

... For data analysis the following populations were gated in sequence. The first gate was Time vs. SSC-A to detect dif- ferences in the flow. To exclude dead cells, the EMA-nega- tive population was selected ...

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Quantitative flow cytometric analysis of opsonophagocytosis and killing of nonencapsulated Haemophilus influenzae by human polymorphonuclear leukocytes.

Quantitative flow cytometric analysis of opsonophagocytosis and killing of nonencapsulated Haemophilus influenzae by human polymorphonuclear leukocytes.

... To discriminate between bacteria internalized by PMNs and bacteria adhering to PMNs, ethidium bromide (50 ,ug/ml) was added to the incubation mixture after phagocytosis, the fluo- rescen[r] ...

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Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes.

Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes.

... fluorescence emitted by the positive PMNL was of lower in- tensity. Along with the deficit of the neonatal PMNL oxidative burst, neonatal PMNL show a consistent chemotactic defect (18, 23, 26). CD11b (CR3) is a member of ...

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Review Articles. Flow Cytometric Analysis of Lymphomas. Current Status and Usefulness. Zahid Kaleem, MD

Review Articles. Flow Cytometric Analysis of Lymphomas. Current Status and Usefulness. Zahid Kaleem, MD

... clonality analysis providing objective and quantitative re- sults even on very small ...where analysis depends on eval- uation of single antigens, usually one at a time, with a dose of subjectivity in ...

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