fluorescein isothiocyanate
Blood–brain barrier transport studies, aggregation, and molecular dynamics simulation of multiwalled carbon nanotube functionalized with fluorescein isothiocyanate
... with fluorescein isothiocyanate; FITC, fluorescein isothiocyanate; BBB, blood–brain barrier; TER, transendothelial electric resistance; SD, standard deviation; h, ...
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Nonlinear Intestinal Absorption of Fluorescein Isothiocyanate Dextran 4, 000 Caused by Absorptive and Secretory Transporting System
... of fluorescein isothiocyanate dextran 4,000 (FD- 4; MW 4,400 Da), which is considered to be an impor- tant paracellular probe for assessing transport in the se- rosal to mucosal direction across rat colonic ...
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Physicochemical and serological characteristics of respiratory virus fluorescein isothiocyanate conjugates for fluorescent antibody diagnosis
... Fluorescein-isothiocyanate FITC conjugates were prepared by improved methods from standard reference antisera to influenza A and B, mumps, parainfluenza 1, 2, 3, and 4, herpesvirus, resp[r] ...
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Honey induced protein stabilization as studied by fluorescein isothiocyanate fluorescence
... Therefore, fluorescein isothiocyanate (FITC) flu- orescence has been used to study protein stabilization against urea and thermal denaturations in the presence of honey using FITC fluorescence spectroscopic ...
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Epigenetics of cell free plasma DNA for non invasive prenatal diagnosis of fetal aneuploidies
... ES cells: Embryonic stem cells FNRBCs: Fetal nucleated red blood cells FISH: Fluorescent in situ hybridisation FITC: Fluorescein isothiocyanate GNAS1: Guanine nucleotide binding protein [r] ...
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Proteasome inhibitors act as bifunctional antagonists of human immunodeficiency virus type 1 latency and replication
... ELISA: Enzyme-linked immunosorbent assay; Env: Envelope; FBS: Fetal bovine serum; FDA: Food and drug administration; FITC: Fluorescein isothiocyanate; GAPDH: Glyceraldehyde 3-phosphate d[r] ...
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Inhibitory effect of ribbon type NF κB decoy oligodeoxynucleotides on osteoclast induction and activity in vitro and in vivo
... FCS = fetal calf serum; FITC = fluorescein isothiocyanate; IL = interleukin; M-CSF = macrophage colony-stimulating factor; NF-κB = nuclear factorκB; ODN = oligodeoxynucleotide; PBS = pho[r] ...
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Restoration of regulatory and effector T cell balance and B cell homeostasis in systemic lupus erythematosus patients through vitamin D supplementation
... FITC: fluorescein isothiocyanate; FoxP3: forkhead box P3; GSEA: gene set enrichment analysis; ICA: independent component analysis; IFN: interferon; IL: interleukin; 25(OH)D: 25-hydroxyvitamin D; PBMC: ...
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Mesenchymal stromal cells from myelodysplastic and acute myeloid leukemia patients display in vitro reduced proliferative potential and similar capacity to support leukemia cell survival
... FITC: Fluorescein isothiocyanate; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; HD: Healthy donor; HD-MSC: MSC isolated from healthy donors; HSC: Hematopoietic stem cell; MCGS: Mesenchymal cell growth ...
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Recombinant RGD-disintegrin DisBa-01 blocks integrin αvβ3 and impairs VEGF signaling in endothelial cells
... FITC: Fluorescein Isothiocyanate; HUVECs: Human Umbilical Vein Endothelial Cell; MMPs: Matrix Metalloproteinases; MTT: (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); OSCC: Oral Squamous ...
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An Indirect Immunoassay for Detecting Antigen Based on Fluorescence Resonance Energy Transfer
... antigen. Fluorescein isothiocyanate (FITC) was attached to anti-BSA antibody (anti-BSA–FITC) as FRET donor, while BSA was conjugated to gold nanoparticles (GNPs–BSA) as FRET ...
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Role of p38 Signaling Pathway in Pentagastrin Regulated Cell Proliferation of Colorectal Carcinoma Cell Line HT 29
... V-fluorescein isothiocyanate (FITC) flow cytometry to determine the proliferation index (PI) and apoptosis rate (AR) of HT-29 cells; reverse transcriptase polymerase chain reaction (RT-PCR) to detect ...
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Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis
... PTX with more than 98% purity was purchased from Yew Biotechnology Co. Ltd. (Jiangsu, China). Taxol (marked product of PTX) was purchased from Bristol-Myers Squibb (China) Investment Co. Ltd. (Shanghai, China). β-CN (No. ...
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P-MAPA immunotherapy potentiates the effect of cisplatin on serous ovarian carcinoma through targeting TLR4 signaling
... FITC: Fluorescein Isothiocyanate; H&E: Hematoxylin and Eosin staining; HRP-conjugated: Horseradish peroxidase-conjugated; IFN: Interferon; IFN- γ : Interferon gamma; IHC: Immunohistochemistry; IkB: I ...
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Fungus-mediated biological synthesis of gold nanoparticles: potential in detection of liver cancer
... ( A and A1 ) liver tumor cells only ( B and B1 ) Nonspecific antibody incubated with liver cancer cell captured by using phase contrast and fluorescein isothiocyanate filter, ( C [r] ...
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Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy
... and fluorescein isothiocyanate-conjugated mouse anti-dextran mab (C) for one hour to detect the human Igg molecules and dextran-coated magnetic nanoparticles, respectively, on the surface of a431 ...
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Role of preterminal protein processing in adenovirus replication.
... The second antibody was fluorescein isothiocyanate FITC-conjugated goat anti-mouse immunoglobulin Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest A direct consequence of[r] ...
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Functional analysis of a murine monoclonal antibody against the repetitive region of the fibronectin binding adhesins fibronectin binding protein A and fibronectin binding protein B from Staphylococcus aureus
... Abbreviations FITC, fluorescein isothiocyanate; Fn, fibronectin; FnBPA, fibronectin-binding protein A; FnBPB, fibronectin-binding protein B; FnBR, fibronectin-binding repeat; FnBRA, fibr[r] ...
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A reduced graphene oxide-based fluorescence resonance energy transfer sensor for highly sensitive detection of matrix metalloproteinase 2
... [nrGO]/fluorescein isothiocyanate-labeled peptide [Pep-FITC]) for ultrasensitive detection of matrix metalloprotei- nase 2 (MMP2) has been developed by engineering the Pep-FITC comprising the specific MMP2 ...
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Pegylation of poly(γ-benzyl-L-glutamate) nanoparticles is efficient for avoiding mononuclear phagocyte system capture in rats
... PBLG-fluorescein isothiocyanate and imaged by fluorescence microscopy to measure their accumulation in liver and spleen tissues of rats after intravenous ...
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